Wnt Signaling: Volume 1: Pathway Methods and Mammalian Models (Methods in Molecular Biology, 468)

Since their discovery, Wnt signaling molecules have been shown to control key events in embryogenesis, to maintain tissue homeostasis in the adult and, when aberrantly activated, to promote human degenerative diseases and cancer, thus making them a vital area of study. __Wnt Signaling: Methods and Protocols__ examines both biochemical assays and vertebrate and invertebrate model systems to provide a point of reference to current molecular protocols and the diverse model systems employed to study this important signaling pathway. In __Volume 1, Pathway Methods and Mammalian Models__, assays to measure activation of the diverse Wnt signaling pathways are explored, along with a selection of models and strategies used to study mammalian Wnt/FZD fuction, from protein-protein interaction and simple cell line models to organoid cultures and mouse models. As a volume in the highly successful __Methods in Molecular BiologyTM__ series, chapters contain readily reproducible laboratory protocols, complete with lists of necessary equipment and reagents and the Notes section, which reveals helpful troubleshooting tips. Comprehensive and cutting-edge, __Wnt Signaling: Methods and Protocols__ collects the expertise and knowledge of many leaders in the field to produce this invaluable two-volume resource.

since Their Discovery, Wnt Signaling Molecules Have Been Shown To Control Key Events In Embryogenesis, To Maintain Tissue Homeostasis In The Adult And, When Aberrantly Activated, To Promote Human Degenerative Diseases And Cancer, Thus Making Them A Vital Area Of Study. Wnt Signaling: Methods And Protocols Examines Both Biochemical Assays And Vertebrate And Invertebrate Model Systems To Provide A Point Of Reference To Current Molecular Protocols And The Diverse Model Systems Employed To Study This Important Signaling Pathway. In Volume 1, Pathway Methods And Mammalian Models, Assays To Measure Activation Of The Diverse Wnt Signaling Pathways Are Explored, Along With A Selection Of Models And Strategies Used To Study Mammalian Wnt/fzd Fuction, From Protein-protein Interaction And Simple Cell Line Models To Organoid Cultures And Mouse Models. As A Volume In The Highly Successful Methods In Molecular Biology™ Series, Chapters Contain Readily Reproducible Laboratory Protocols, Complete With Lists Of Necessary Equipment And Reagents And The Notes Section, Which Reveals Helpful Troubleshooting Tips.

comprehensive And Cutting-edge, Wnt Signaling: Methods And Protocols Collects The Expertise And Knowledge Of Many Leaders In The Field To Produce This Invaluable Two-volume Resource.

Since their discovery, Wnt signaling molecules have been shown to control key events in embryogenesis, to maintain tissue homeostasis in the adult and, when aberrantly activated, to promote human degenerative diseases and cancer, thus making them a vital area of study. Wnt Signaling examines both biochemical assays and vertebrate and invertebrate model systems to provide a point of reference to current molecular protocols and the diverse model systems employed to study this important signaling pathway. In Volume 2, Pathway Models, the diverse vertebrate and invertebrate models that have shaped the Wnt signaling field are described, presenting an overview of the unique properties of each organism, like asymmetric cell division in C. elegans and epithelial morphogenesis in Dictyostelium, with respect to studying Wnt/FZD function. As a volume in the highly successful Methods in Molecular Biology series, chapters contain readily reproducible laboratory protocols, complete with lists of necessary equipment and reagents and the Notes section, which reveals helpful troubleshooting tips Front Matter....Pages i-xv Back Matter....Pages 5-15 ....Pages 17-29 v. 1. Pathway methods and mammalian models v. 2. Pathway models.