Vitamins and Coenzymes, Part H, Volume 123: Volume 123: Vitamins and Coenzymes Part H (Methods in Enzymology)

The critically acclaimed laboratory standard, **Methods in Enzymology**, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences Content: Contributors to volume 123 Pages ix-xii Preface Page xiii Frank Chytil, Donald B. McCormick Volumes in series Pages xv,xvii-xxvi [1] Determination of cobalamins in biological material Original Research Article Pages 3-14 Peter Gimsing, William S. Beck [2] Analysis of cobalamin coenzymes and other corrinoids by high-performance liquid chromatography Original Research Article Pages 14-22 Donald W. Jacobsen, Ralph Green, Kenneth L. Brown [3] Isolation of native and proteolytically derived ileal receptor for intrinsic factor—cobalamin Original Research Article Pages 23-28 Bellur Seetharam, David H. Alpers [4] Purification of B 12 -binding proteins using a photodissociative affinity matrix Original Research Article Pages 28-36 D.W. Jacobsen, F.M. Huennekens [5] Solid-phase immunoassay for human transcobalamin II and detection of the secretory protein in cultured human cells Original Research Article Pages 36-49 Marijke FrГ ter-SchrГ¶der [6] Quantitative analysis of retinal and 3-dehydroretinal by high-pressure liquid chromatography Original Research Article Pages 53-61 Momoyo Makino-Tasaka, Tatsuo Suzuki [7] Enzymatic synthesis and separation of retinyl phosphate mannose and dolichyl phosphate mannose by anion-exchange high-performance liquid chromatography Original Research Article Pages 61-68 Kim E. Creek, Donata Rimoldi, Michele Brugh-Collins, Luigi M. De Luca [8] Separation and quantitation of retinyl esters and retinol by high-performance liquid chromatography Original Research Article Pages 68-74 A. Catharine Ross [9] Separation of geometric isomers of retinol and retinoic acid in nonaqueous high-performance liquid chromatography Original Research Article Pages 75-85 Pangala V. Bhat, Andre Lacroix [10] Use of enzyme-linked immunosorbent assay technique for quantitation of serum retinol-binding protein Original Research Article Pages 85-92 Nobuo Monji, Ejal Bosin [11] Fourier transform infrared spectroscopy of retinoids Original Research Article Pages 92-101 Natalie L. Rockley, Mark G. Rockley, Bruce A. Halley, Eldon C. Nelson [12] Purification of interstitial retinol-binding protein from the eye Original Research Article Pages 102-111 S.-L. Fong, G.I. Liou, C.D.B. Bridges [13] Quantification of physiological levels of retinoic acid Original Research Article Pages 112-124 Joseph L. Napoli [14] Isolation and identification of vitamin D metabolites Original Research Article Pages 127-140 Joseph L. Napoli, Nick J. Koszewski, Ronald L. Horst [15] A new pathway of 25-hydroxyvitamin D 3 metabolism Original Research Article Pages 141-154 Glenville Jones [16] Enzyme-linked immunoabsorption assay for vitamin D-induced calcium-binding protein Original Research Article Pages 154-159 Barbara E. Miller, Anthony W. Norman [17] Measurement of 25-hydroxyvitamin D 1О±-hydroxylase activity in mammalian kidney Original Research Article Pages 159-167 Bruce Lobaugh, Janice R. Almond, Marc K. Drezner [18] Quantitation of vitamin D 2 , vitamin D 3 , 25-hydroxyvitamin D 2 , and 25-hydroxyvitamin D 3 in human milk Original Research Article Pages 167-176 Bruce W. Hollis, Nancy E. Frank [19] 1,25-dihydroxyvitamin D microassay employing radioreceptor techniques Original Research Article Pages 176-185 Timothy A. Reinhardt, Bruce W. Hollis [20] Assay for 1,25-dihydroxyvitamin D using rabbit intestinal cytosol-binding protein Original Research Article Pages 185-190 W.E. Duncan, T.C. Aw, J.G. Haddad [21] Cytoreceptor assay for 1,25-dihydroxyvitamin D Original Research Article Pages 190-198 Stavros C. Manolagas [22] Monoclonal antibodies as probes in the characterization of 1,25-dihydroxyvitamin D 3 receptors Original Research Article Pages 199-211 J. Wesley Pike, Mark R. Haussler [23] An HPLC method for the simultaneous determination of retinol and О±-tocopherol in plasma or serum Original Research Article Pages 215-219 G.L. Catignani [24] Assay of coumarin antagonists of vitamin K in blood by high-performance liquid chromatography Original Research Article Pages 223-234 Martin J. Shearer [25] Assay of K vitamins in tissues by high-performance liquid chromatography with special reference to ultraviolet detection Original Research Article Pages 235-251 Martin J. Shearer [26] Analysis of bacterial menaquinone mixtures by reverse-phase high-performance liquid chromatography Original Research Article Pages 251-256 Jin Tamaoka [27] Radioisotopic assay of acetylcarnitine and acetyl-CoA Original Research Article Pages 259-263 Shri V. Pande [28] Short-chain acylcarnitines: Identification and quantitation Original Research Article Pages 264-276 L.L. Bieber, J. Kerner [29] Purification and assay of carnitine acyltransferases Original Research Article Pages 276-284 L.L. Bieber, C. Fiol [30] Determination of the specific activity of long-chain acylcarnitine esters Original Research Article Pages 284-290 Richard Odessey [31] Synthesis of carnitine precursors and related compounds Original Research Article Pages 290-297 Charles J. Rebouche [32] Synthesis of radioactive (в€’)-carnitine from Оі-aminobutyrate Original Research Article Pages 297-302 Shri V. Pande [33] S -adenosylmethionine: Оμ- N - l -lysine methyltransferase Original Research Article Pages 303-308 Harry P. Broquist [34] Reflectance spectrophotometric and surface fluorometric methods for measuring the redox state of nicotinamide nucleotides and flavins in intact tissues Original Research Article Pages 311-320 Ilmo E. Hassinen [35] Spectrophotometric determination of iron in heme proteins Original Research Article Pages 320-323 Serge N. Vinogradov [36] Affinity chromatography of heme-binding proteins: synthesis and characterization of hematin- and hematoporphyrin-agarose Original Research Article Pages 324-331 Kenneth W. Olsen [37] Affinity chromatography of heme-binding proteins: synthesis of hemin-agarose Original Research Article Pages 331-338 Ken Tsutsui [38] Porphobilinogen synthase: a specific and sensitive coupled-enzyme assay Original Research Article Pages 339-345 David F. Bishop, Robert J. Desnick [39] High-performance liquid chromatographic analysis of porphyrins and their isomers with radial compression columns Original Research Article Pages 346-352 Andreas Seubert, Sigrid Seubert [40] High-performance liquid chromatography of porphyrin methyl esters Original Research Article Pages 352-363 James G. Straka [41] Synthesis of [ 14 C]Coproporphyrin III by yeast cell-free extracts Original Research Article Pages 363-370 Pierre Labbe [42] Isotachophoretic analysis of porphyrin precursors: 5-aminolevulinic acid derivatives Original Research Article Pages 370-374 Yuzo Shioi, Tsutomu Sasa [43] Preparation of stereospecifically labeled porphobilinogens Original Research Article Pages 375-383 M. Akhtar, C. Jones [44] High-performance liquid chromatography of uroporphyrin and coproporphyrin isomers Original Research Article Pages 383-389 C.K. Lim, T.J. Peters [45] High-performance liquid chromatographic separation of the bilirubin isomers Original Research Article Pages 389-395 C.K. Lim, T.J. Peters [46] Purification of hepatic mitochondrial 5-aminolevulinate synthase Original Research Article Pages 395-401 Iain A. Borthwick, Gopesh Srivastava, Byron A. Pirola, Brian K. May, William H. Elliott [47] Purification and characterization of mammalian and chicken ferrochelatase Original Research Article Pages 401-408 Harry A. Dailey, Jennie E. Fleming, Bertille M. Harbin [48] Purification and characterization of bacterial ferrochelatase Original Research Article Pages 408-415 Harry A. Dailey [49] Uroporphyrinogen decarboxylase purification from chicken erythrocytes Original Research Article Pages 415-421 Yoshihiko Seki, Shosuke Kawanishi, Seiyo Sano [50] Purification of solubilized chlorophyllase from Chlorella protothecoides Original Research Article Pages 421-427 Yuzo Shioi, Tsutomu Sasa [51] Purification of porphobilinogen synthase from bovine liver Original Research Article Pages 427-434 Peter M. Jordan, Jasbir S. Seehra [52] Purification of 5-aminolevulinate synthase Original Research Article Pages 435-443 Peter M. Jordan, Aghdas Laghai-Newton Author index Pages 445-460 Subject index Pages 461-476 This volume of Methods in Enzymology and its companion volumes 280, 281, and 282 present new methods and their modifications developed in the past decade plus the more recently acquired knowledge on the functional and metabolic aspects of vitamins and coenzymes. They represent the only in-depth treatment dealing with methods related to vitamins and coenzymes, and contain methods that have never been grouped together before. Key Features * This volume and its companion Volumes 280, 281, and 282 provide * A collation of the most recent and useful methods for the identfication, preparation, and quantification of vitamins and coenzymes * Details on physical, chemical, and biological properties of vitamins and coenzymes * Chemical and biological syntheses of vitamins, coenzymes, and their analogs * Aspects of transport and metabolism of vitamins and coenzymes