Viral Genome Methods

Molecular biology and genetics techniques now dominate viral research in attempts to cure diseases such as AIDS. Viral Genome Methods is a practical guide to the newest molecular techniques, providing step-by-step protocols to be used in the laboratory. Recognized authorities and pioneers in viral research pass on their expertise to you. Cover Half Title Title Copyright Preface The Editor Contributors Contents Section I. Retroviruses/HIV/RNA Viruses Chapter 1. Retrovirus-Mediated Gene Transfer Chapter 2. Methods for Studying Genetic Variation of the Human Immunodeficiency Virus (HIV) Chapter 3. Methods for HIV/SIV Gene Analysis Chapter 4. Analysis of Human Immunodeficiency Virus Reverse Transcriptase Function Chapter 5. Tat Activation Studies: Use of a Reporter Cell Line Chapter 6. Characterization of HIV Nef Cell-Specific Effects Chapter 7. Detection, Quantitation, and Characterization of Human Retroviruses Chapter 8. Quantitative Methods to Monitor Viral Load in Simian Immunodeficiency Virus Infections Chapter 9. Using Molecular Genetics to Probe the Immune Response to Vesicular Stomatitis Virus Section II. DNA Viruses Chapter 10. Transient Genetic Approaches to Studying Multifactor Processes in Complex DNA Viruses Chapter 11. Epstein-Barr Virus: Gene Expression and Regulation Chapter 12. Functional Analysis of the Herpes Simplex Virus DNA Polymerase Chapter 13. Molecular Genetics of Adeno-Associated Virus Chapter 14. Reconstitution of Adenovirus DNA Replication with Purified Replication Proteins Section III. Index Edited By Kenneth W. Adolph. Includes Bibliographical References And Index.