Understanding PCR : A Practical Bench-Top Guide

__Understanding PCR: A Practical Bench-Top Guide__ gives you all of the information you need to plan your first PCR, from reagents to conditions to analysis and beyond. It is a user friendly book that has step-by-step basic protocols, which can be adapted to your needs. Includes helpful information such as where to order your reagents and basic troubleshooting hints and tips. * Includes resources for reagents * Explains basic laboratory preparation * Provides straightforward experimental protocols * Incorporates fundamental analytical techniques * Contains a troubleshooting guide Content: Front Matter,Copyright,Author BiographiesEntitled to full textChapter 1 - Things to Consider Before Preparing to Undertake Your First Polymerase Chain Reaction, Pages 1-9 Chapter 2 - Designing and Ordering Your Polymerase Chain Reaction Primers, Pages 11-30 Chapter 3 - Analyzing Your Template DNA and/or PCR Product, Pages 31-43 Chapter 4 - Quantitative PCR: Things to Consider, Pages 45-52 Chapter 5 - Carrying Out Q-PCR, Pages 53-59 Chapter 6 - Using PCR for Cloning and Protein Expression, Pages 61-71 Chapter 7 - Polymerase Chain Reaction for Knocking Out Genes, Pages 73-79 Appendix 1, Pages 81-82 Appendix 2, Page 83 Index, Pages 85-87

Understanding PCR: A Practical Bench-Top Guide gives you all of the information you need to plan your first PCR, from reagents to conditions to analysis and beyond. It is a user friendly book that has step-by-step basic protocols, which can be adapted to your needs. Includes helpful information such as where to order your reagents and basic troubleshooting hints and tips.

• Includes resources for reagents
• Explains basic laboratory preparation
• Provides straightforward experimental protocols
• Incorporates fundamental analytical techniques
• Contains a troubleshooting guide

Authors: Sarah Maddocks And Rowena Jenkins. Includes Bibliographical References And Index.