Ultrasensitive and Rapid Enzyme Immunoassay (Volume 27) (Laboratory Techniques in Biochemistry and Molecular Biology, Volume 27)

Immunoassay techniques have become essential in various fields of pure and applied research. This volume of the well known Laboratory Techniques series will be of assistance to those who have plans or are making efforts to develop ultrasensitive enzyme immunoassays for antigens and antibodies. The volume describes factors limiting the sensitivity of noncompetitive solid phase enzyme immunoassays, methods to overcome difficulties limiting sensitivities, methods to perform ultrasensitive enzyme immunoassays as rapidly as possible, and protocols of enzyme labeling and enzyme assays as well as ultrasensitive enzyme immunoassays. Ultrasensitive and Rapid Enzyme Immunoassay, with its clear presentation and up-to-date information, will be essential to scientists in pharmaceutical companies and in applied research. v. 1. pt. 1. Electrophoresis of proteins in polyacrylamide and starch gels, by A.H. Gordon.- pt. 2. An introduction to gel chromatography, by L. Fischer.- pt. 3. Immunochemical techniques for the identification and estimation of macromolecules, by J. Clausen. v. 2. pt. 1. Automated enzyme assays, by D.B. Roodyn.- pt. 2. Cellulosic ion exchangers, by E.A. Peterson. v. 3. pt. 1. Determination of sequences in RNA, by G.G. Brownlee.- pt. 2. Techniques of lipidology, by M. Kates. v. 4. pt. 1. Chemical modification of proteins, by A.N. Glazer, R.J. DeLange and D.S. Sigman.- pt. 2. Separation methods for nucleic acids and oligonucleotides, by H. Gould and H.R. Matthews. v. 5. pt. 1. Techniques of sample preparation of liquid scintillation counting, by B.W. Fox.- Isoelectric focusing, by P.G. Righetti and J.W. Drysdale. v. 6. pt. 1. Density gradient centrifugation, by R. Hinton and M. Dobrota.- pt. 2. An introduction to radioimmunoassay and related techniques, by T. Chard. v. 7, pt. 1. Preparation and characterisation of mammalian plasma membranes, by W.H. Evans. pt. 2. An introduction to affinity chromatography, by C.R. Lowe. v. 8. Cell culture for biochemists, by R.L.P. Adams. v. 9. Sequencing of proteins and peptides, by G. Allen. v. 10. DNA sequencing, [by] J. Hindley. v. 11. Isoeletric focusing: theory, methodology and applications, [by] P. G. Righetti. v. 12. Photogenerated reagents in biochemistry and molecular biology, [by] H. Bayley. v. 13. Monoclonal antibody technology: the production and characterization of rodent and human hybridomas, [by] A.M. Campbell. v. 14. A guidebook to lipoprotein technique, [by] G.L. Mills, P.A. Lane, and P.K. Weech. v. 15. Practice and theory of enzyme immunoassays, [by] P. Tijssen. v. 16. Glycoprotein and proteoglycan techniques, [by] J.G. Beeley. v. 17. Applications of hplc in biochemistry, [by] A. Fallon, R.F.G. Booth and L.D. Bell. v. 18. Methods of cell separation, [by] P.T. Sharpe. v. 19. Synthetic polypeptides as antigens [by] M.H.V. Van Regenmortel [et al.]. v. 20. Immobilized pH gradients: theory and methodology, by] P. G. Righetti. v. 21. Isolated hepatocytes preparation, properties and applications by M.N. Berry, A.M. Edwards [and] G.J. Barritt. v. 22. Techniques in free radical research, edited by R.H. Burdon [and] P.H. van Knippenberg. v. 23. Monoclonal antibody and immunosensor technology, edited by P.C. van der Vliet. v. 24. Hybridization with nucleic acid probes.- pt. 1. Theory and nucleic acid preparation.- pt. 2. Probe labeling and hybridization techniques. v. 25. Dry chemistry. v. 26. Analysis of RNA-protein complexes in Vitro v. 27. Ultrasensitive and rapid enzyme immunoassay / Eiji Ishikawa v. 28. Synthetic peptides as antigens / M.H.V. Van Regenmortel, S. Muller. v. 29. Cancer metastasis: in vitro and in vivo experimental approaches v. 30. Inositol phospholipid metabolism and phosphatidyl inositol kinases v. 32. Magnetic cell separation. Content: Edited by Page ii Copyright page Page iv Preface Page v Eiji Ishikawa Chapter 1 Introduction: classification of immunoassays Pages 1-3 Chapter 2 History of ultrasensitive enzyme immunoassay Pages 5-6 Chapter 3 Factors limiting the sensitivity of noncompetitive heterogeneous solid phase enzyme immunoassays Pages 7-16 Chapter 4 Methods to minimize effects of factors limiting the sensitivity Pages 17-77 Chapter 5 Principle of the immune complex transfer enzyme immunoassay Pages 79-95 Chapter 6 Potential of the immune complex transfer enzyme immunoassay to improve the sensitivity and its limitations Pages 97-140 Chapter 7 Ultrasensitive and rapid enzyme immunoassay Pages 141-176 Chapter 8 Protocol of immune complex transfer enzyme immunoassay Pages 177-191 Chapter 9 Ultrasensitive immunoassay for haptens Pages 193-210 Chapter 10 Properties of the reagents for enzyme-labeling Pages 211-221 Chapter 11 Enzyme-labeling of antibodies Pages 223-248 Chapter 12 Preparation and affinity-purification of IgG fragments Pages 249-259 Chapter 13 Protocol for enzyme-labeling of antibodies Pages 261-275 Chapter 14 Protocol for labeling avidin with 2,4-dinitrophenyl groups and β-D-galactosidase Pages 277-282 Chapter 15 Enzyme-labeling of antigens Pages 283-287 Chapter 16 Protocol for enzyme-labeling of antigens Pages 289-302 Chapter 17 Assays of enzymes Pages 303-309 References Pages 311-325 Subject Index Pages 327-334 Immunoassay techniques have become essential in various fields of pure and applied research. This volume in the andquot;Laboratory Techniquesandquot; series should be of useful to those studying or developing ultrasensitive enzyme immunoassays for antigens and antibodies. The volume describes factors limiting the sensitivity of noncompetitive solid phase enzyme immunoassays, methods to overcome difficulties limiting sensitivities, methods to perform ultrasensitive enzyme immunoassays as rapidly as possible, and protocols of enzyme labelling and enzyme assays as well as ultrasensitive enzyme im Immunoassay techniques have become essential in various fields of pure and applied research. This guide describes many of the techniques used in this field, how to avoid and overcome obstacles encountered, and some of the theory behind the methods