Tubulin-Binding Agents: Synthetic, Structural and Mechanistic Insights (Topics in Current Chemistry Book 286)

reviews. The SAR data are discussed in light of the structural information available for each agent. The second chapter focuses on the total synthesis of the marine sponge-derived polyketide discodermolide. A comprehensive survey of the synthetic chemistry efforts of several groups over a 14-year period is provided together with a compa- son of the different approaches. The third chapter describes a comprehensive study of the mechanisms of activity of microtubules stabilizing drugs. Thermodynamic, kinetic, structural and fu- tional data on microtubules stabilizing drugs are discussed in an interdisciplinary manner to generate a “time-resolved” picture of the interaction of the drugs with different tubulin forms. The fourth and fifth chapters review the efforts and achievements made in the characterization of the structure of the complexes of tubulin with microtubules stabilizing agents by NMR (Chapter 4) and EM (Chapter 5). Especially evident is the discrepancy of the results obtained for epothilones, where the two techniques deliver radically different structures of the bound drug. Both NMR and EM models are, however, able to explain a consistent set of SAR data. The authors of the two chapters discuss critically the advantages and limitations of each methodology. Front Matter....Pages i-xi Macrolide-Based Microtubule-Stabilizing Agents – Chemistry and Structure–Activity Relationships....Pages 1-72 The Chemical Synthesis of Discodermolide....Pages 73-119 The Interaction of Microtubules with Stabilizers Characterized at Biochemical and Structural Levels....Pages 121-149 The Tubulin Binding Mode of MT Stabilizing and Destabilizing Agents Studied by NMR....Pages 151-208 The Tubulin Binding Mode of Microtubule Stabilizing Agents Studied by Electron Crystallography....Pages 209-257 Microtubule-Destabilizing Agents: Structural and Mechanistic Insights from the Interaction of Colchicine and Vinblastine with Tubulin....Pages 259-278 Molecular Modeling Approaches to Study the Binding Mode on Tubulin of Microtubule Destabilizing and Stabilizing Agents....Pages 279-328 Back Matter....Pages 329-331

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