Transfer and expression of eukaryotic genes : (proceedings of a symposium; New York, 3-5 June 1983)

Transfer and Expression of Eukaryotic Genes documents the progress in our understanding of the transfer and expression of eukaryotic genes. This book covers topics organized around three themes: gene expression and its regulation; in vivo gene transfer and development; and viral gene and oncogene systems. This text is divided into three sections encompassing 25 chapters and begins with an overview of the molecular basis of gene expression, with emphasis on transcription complexes that account for transcription control in eukaryotic genes. It then turns to experiments that assess the in vitro stimulatory effect of the SV40 72-bp repeat on specific transcription from heterologous promoter elements using a HeLa whole cell extract. The reader is methodically introduced to the regulation signals and factors of histone gene transcription; transcriptional control of beta-globin and liver-specific genes in mouse cells; and gene transfer in Drosophila and the sea urchin Strongylocentrotus purpuratus. This book also considers the splicing of messenger RNA precursors and the regulation of thymidine kinase enzyme expression, and then concludes with a chapter that describes the activation of the myc oncogene by chromosomal translocation. This book will be of interest to students and researchers in fields ranging from molecular genetics to microbiology, biochemistry, pathology, and immunology. Content: P & S BIOMEDICAL SCIENCES SYMPOSIA Series , Page ii Front Matter , Page iii Copyright , Page iv List of Participants , Pages xi-xx Sol Spiegelman (1914–1983) , Pages xxi-xxii Preface , Page xxiii On the Molecular Basis of Gene Expression , Pages 3-4 Stimulation of in Vitro Transcription from Heterologous Promoters by the SV40 Enhancer , Pages 7-21 Correct Developmental and Tissue Specific Expression of a Cloned Alcohol Dehydrogenase Gene Introduced into the Drosophila Germ Line by P Element Transformation , Pages 23-37 DNA Methylation and Globin Gene Expression , Pages 39-54 Regulation Signals and Factors of Histone Gene Transcription , Pages 55-63 Transcriptional Control of β-Globin and Liver-Specific Genes in Mouse Cells , Pages 65-77 Transcriptional Enhancement by Specific Regulatory Protein – DNA Complexes , Pages 79-92 Control Elements Located within the Major Intervening Sequences of Murine Kappa Light Chain Genes , Pages 93-104 The Use of Transposable Elements as Vectors for Gene Transfer in Drosophila , Pages 107-111 Gene Transfer in the Sea Urchin Strongylocentrotus purpuratus , Pages 113-121 Position Effects and Gene Expression in the Transgenic Mouse , Pages 123-134 The Transfer of New Genes into Mice , Pages 135-140 Isolation of Human Surface Receptor Genes by Eukaryotic Gene Transfer , Pages 141-142 Nuclear Transplantation and Gene Transfer in Amphibian Eggs and Oocytes , Pages 143-151 The Expression of Neuropeptide Genes in Developing and Adult Neurons , Pages 153-168 Partially Homologous Recombination in Mouse L Cells , Pages 169-179 Splicing of Messenger RNA Precursors , Pages 183-202 Studies on the Regulation of Thymidine Kinase Enzyme Expression , Pages 203-226 The Regulation of α Genes of Herpes Simplex Virus 1 , Pages 227-238 Cis and Trans Acting Regulation of Early Adenovirus Transcription , Pages 239-245 Mutational Analysis of the Functional Domains of the Ad5-E1a Gene Products Effecting Cell Transformation , Pages 247-262 Adenovirus VAI RNA: A Virus-Coded Translational Enhancer , Pages 263-272 Regulation of SV40 Early Transcription , Pages 273-277 Transforming Genes of Chicken and Human B Cell Lymphomas , Pages 279-285 Activation of the myc Oncogene by Chromosomal Translocation , Pages 287-298 Index , Pages 299-304