Topics in Fluorescence Spectroscopy (Topics in Fluorescence Spectroscopy Series): DNA Technology, Vol. 7

During the past 15 years, there has been remarkable progress in the analysis and manipulation of DNA and its use in nanotechnology. DNA analysis is ubiquitous in molecular biology, medical diagnostics, and forensics. Much of the readout technology is based on fluorescence detection. This volume contains contributions from many experts in the field who present an overview of many aspects of DNA technology. These chapters provide an understanding of the underlying principles and technology, rather than an exhaustive review of the literature. Written in a clear straightforward style, this book is an excellent introduction for any scientist to the use of fluorescence in DNA analysis. **DNA Technology** is an essential reading for all academics, bench scientists, and industry professionals wishing to take advantage of the latest and greatest in this continuously emerging field. Key Features: \*Comprehensive overview of the complexities of DNA analysis, \*Covers topics of universal interest to a broad field of scientists, \*Accessible utility in presenting state-of-the-art DNA technology, \*Chapters authored by key figures in the field.

During the past 15 years, there has been remarkable progress in the analysis and manipulation of DNA and its use in nanotechnology. DNA analysis is ubiquitous in molecular biology, medical diagnostics, and forensics. Much of the readout technology is based on fluorescence detection. This volume contains contributions from many experts in the field who present an overview of many aspects of DNA technology. These chapters provide an understanding of the underlying principles and technology, rather than an exhaustive review of the literature. Written in a clear straightforward style, this book is an excellent introduction for any scientist to the use of fluorescence in DNA analysis.

DNA Technology is an essential reading for all academics, bench scientists, and industry professionals wishing to take advantage of the latest and greatest in this continuously emerging field.

Key Features:

*Comprehensive overview of the complexities of DNA analysis,

*Covers topics of universal interest to a broad field of scientists,

*Accessible utility in presenting state-of-the-art DNA technology,

*Chapters authored by key figures in the field.

DNA Sequencing Using Fluorescence Detection....Pages 1-68 Fluorescence in Nucleic Acid Hybridization Assays....Pages 69-103 Energy Transfer Fluorescent Labels for DNA Sequencing and Analysis....Pages 105-127 On-the-Fly Fluorescence Lifetime Detection in Capillary Electrophoresis for DNA Analysis....Pages 129-149 Fluorescent Nucleoside Analogues as DNA Probes....Pages 151-175 Lanthanide-Labeled DNA....Pages 177-212 DNA Arrays for Genetic Analyses and Medical Diagnosis....Pages 213-237 Flow Cytometric Sizing of DNA Fragments....Pages 239-270 Fluorimetric DNA Biosensors....Pages 271-289 Technicolor Genome Analysis....Pages 291-316 v. 1. Techniques v. 2. Principles v. 3. Biochemical applications v. 4. Probe design and chemical sensing v. 5. Nonlinear and two-photon-induced fluorescence v. 6. Protein fluorescence v. 7. DNA technology v. 8. Radiative decay engineering v. 9. Advanced concepts in fluorescence sensing. pt. A. Small molecule sensing v. 10. Advanced concepts in fluorescence sensing. pt. B. Macromolecular sensing Annotation. In this inaugural volume of a new series, experts in the field help biochemists, analytical chemists, spectroscopists, biophysicists, and other specialists keep up with the latest techniques and technologies available in fluorescence spectroscopy The blueprint for all cellular structures and functions is encoded in the genome of any organism.