Tobacco BY-2 Cells: From Cellular Dynamics to Omics (Biotechnology in Agriculture and Forestry Book 58)

It Is Our Utmost Pleasure To Present A New Book On Tobacco By-2 Cells, Tobacco By-2 Cells: From Cellular Dynamics To Omics, As The 58th Volume In The Book Series Biotechnology In Agriculture And Forestry (baf). It Represents An Ext- Sion Of The Previous Book Tobacco By-2 Cells, Volume 53ofthe Baf. Moreover, The Content Is Rather Different From The Latter And Includes New Topics, Gleaned From The First International Symposium On Tobacco By-2 Cells Held At The Plant Science Center Of The Riken, Yokohama, Organized By Nagata, Matsuoka And Inze, In September 2004. To This Symposium Came More Than 200 People From Different Parts Of The World To Discuss Issues. Although Most Of The Contributors To Thepreviousvolumeof Tobacco By-2 Cells Gave Talks On Their Subjects, There Were Many Other Speakers Who Presented New Topics And Approaches. So We Enjoyed The Symposium Very Much. Thus We Decided To Compile A New Volume On Tobacco By-2 Cells Which Includes These New Topics. In Addition, Towards The End Of The Symposium, Our Common Understanding Was That The Tobacco By-2 Cell System Is Still Important In Plant Biology, In Particular For Studying The Dynamic Features Of Plant Cells. We Hope This Volume Is Useful For Plant Biologists. Contents Of The Book Are As Follows: In Chapters I. 1{u2013}i. 6, Various Aspects Of The Cell Cycle And Cellular Dynamics Using By-2 Cells Are Described. In Ch- Ters Ii. 1{u2013}ii. Cell Cycle And Cellular Dynamics -- Novel Approaches For Cell Cycle Analysis In By-2 -- Dynamics And Structure Of The Preprophase Band And The Phragmoplast -- Formation Of Cortical Microtubules In A Cell-free System Prepared From Plasma Membrane Ghosts And A Cytosolic Extract Of By-2 Cells -- Chromosome Dynamics In Tobacco By-2 Cultured Cells -- Ion Channels Meet Cell Cycle Control -- The Nack-pqr Map Kinase Cascade Controls Plant Cytokinesis -- Physiological And Developmental Aspects -- Characterization Of A Cell Division Factor From Auxin-autotrophic 2b-13 Cells Derived From The Tobacco By-2 Cell Line -- The By-2 Cell Line As A Tool To Study Auxin Transport -- Tobacco By-2 Cells As A Model For Differentiation In Heterotrophic Plant Cells -- Intracellular Traffic -- Imaging The Early Secretory Pathway In By-2 Cells -- Molecular Study Of Prevacuolar Compartments In Transgenic Tobacco By-2 Cells -- Autophagy And Non-classical Vacuolar Targeting In Tobacco By-2 Cells --^ As A Host For Infectious Diseases -- In Vitro Translation And Replication Of Tobamovirus Rna In A Cell-free Extract Of Evacuolated Tobacco By-2 Protoplasts -- Using By-2 Cells To Investigate Agrobacterium-plant Interactions -- Regulation Of Elicitor-induced Defense Responses By Ca2+ Channels And The Cell Cycle In Tobacco By-2 Cells -- Other Cellular Functions -- Dynamic Mitochondria, Their Fission And Fusion In Higher Plants -- The Use Of Tobacco By-2 Cells To Elucidate The Biosynthesis And Essential Functions Of Isoprenoids -- Omics -- Tobacco By-2 Proteome Display, Protein Profiling And Annotation Using Two-dimensional Electrophoresis And Mass Spectrometry-based Cross-species Identification -- Est And Microarray Analysis Of Tobacco By-2 Cells -- Proteomics Of Tobacco Bright Yellow-2 (by-2) Cell Culture Plastids -- Technical Advances -- Cryopreservation Of Tobacco By-2 Suspension Cell Cultures Using An Encapsulation {u2014} Simple Prefreezing Method --^ High Throughput Microinjection Technology For The Single-cell Analysis Of By-2 In Vivo. Edited By T. Nagata, K. Matsuoka And D. Inze. Includes Bibliographical References And Index. Mode Of Access: World Wide Web. Front Matter....Pages 1-1 Novel Approaches for Cell Cycle Analysis in BY-2....Pages 3-21 Dynamics and Structure of the Preprophase Band and the Phragmoplast....Pages 23-40 Formation of Cortical Microtubules in a Cell-Free System Prepared from Plasma Membrane Ghosts and a Cytosolic Extract of BY-2 Cells....Pages 41-49 Chromosome Dynamics in Tobacco BY-2 Cultured Cells....Pages 51-63 Ion Channels Meet Cell Cycle Control....Pages 65-78 The NACK-PQR MAP Kinase Cascade Controls Plant Cytokinesis....Pages 79-94 Front Matter....Pages 95-95 Characterization of a Cell Division Factor from Auxin-Autotrophic 2B-13 Cells Derived from the Tobacco BY-2 Cell Line....Pages 97-106 The BY-2 Cell Line as a Tool to Study Auxin Transport....Pages 107-117 Tobacco BY-2 Cells as a Model for Differentiation in Heterotrophic Plant Cells....Pages 119-132 Front Matter....Pages 133-133 Imaging the Early Secretory Pathway in BY-2 Cells....Pages 135-151 Molecular Study of Prevacuolar Compartments in Transgenic Tobacco BY-2 Cells....Pages 153-166 Autophagy and Non-Classical Vacuolar Targeting in Tobacco BY-2 Cells....Pages 167-180 Front Matter....Pages 181-181 In Vitro Translation and Replication of Tobamovirus RNA in a Cell-Free Extract of Evacuolated Tobacco BY-2 Protoplasts....Pages 183-194 Using BY-2 Cells to Investigate Agrobacterium -Plant Interactions....Pages 195-206 Regulation of Elicitor-Induced Defense Responses by Ca 2+ Channels and the Cell Cycle in Tobacco BY-2 Cells....Pages 207-221 Front Matter....Pages 223-223 Dynamic Mitochondria, their Fission and Fusion in Higher Plants....Pages 225-240 The Use of Tobacco BY-2 Cells to Elucidate the Biosynthesis and Essential Functions of Isoprenoids....Pages 241-272 Front Matter....Pages 273-273 Tobacco BY-2 Proteome Display, Protein Profiling and Annotation Using Two-Dimensional Electrophoresis and Mass Spectrometry-Based Cross-Species Identification....Pages 275-292 EST and Microarray Analysis of Tobacco BY-2 Cells....Pages 293-311 Proteomics of Tobacco Bright Yellow-2 (BY-2) Cell Culture Plastids....Pages 313-326 Front Matter....Pages 327-327 Cryopreservation of Tobacco BY-2 Suspension Cell Cultures Using an Encapsulation — Simple Prefreezing Method....Pages 329-337 High Throughput Microinjection Technology for the Single-Cell Analysis of BY-2 in Vivo....Pages 339-346 The tobacco BY-2 cell system is a unique model cell line for the study of dynamic features of plant cells. As extension of Volume 53, Tobacco BY-2 Cells, which presented basic aspects of the cell system, this present volume provides a wealth of new approaches. This latest volume in the series is an invaluable source of information for scientists in basic and applied plant biology.