Tissue Engineering Methods and Protocols

Jeffrey Morgan and Martin Yarmush bring together in Tissue Engineering the first major collection of cutting-edge methods developed and used by leading researchers in the tissue engineering field. These hands-on experts describe easily reproducible protocols for the production and evaluation of polymers, scaffolds, and composites, as well as methods for the isolation, culture, and analysis of cells, including the combination of cells with a variety of materials or devices. Quantitative methods designed to assess cell performance and cell function that will also illuminate our understanding of cellular processes and the cellular response to the chemical and mechanical environment of a functional organ/tissue are also offered. Tissue Engineering provides researchers with detailed methods and protocols covering a comprehensive range of key technologies and techniques used by leaders in tissue engineering research. Its interdisciplinary methods-drawn from the life sciences, engineering, and clinical medicine-are already making a significant contribution to the development of new and effective treatments for both organ and tissue defects. Jeffrey Morgan and Martin Yarmush bring together in Tissue Engineering Methods and Protocols the first major collection of cutting-edge methods developed and used by leading researchers in the tissue engineering field. These hands-on experts describe easily reproducible protocols for the production and evaluation of polymers, scaffolds, and composites, as well as methods for the isolation, culture, and analysis of cells, including the combination of cells with a variety of materials or devices. Quantitative methods designed to assess cell performance and cell function that will also illuminate our understanding of cellular processes and the cellular response to the chemical and mechanical environment of a functional organ/tissue are also offered.

The book contains black-and-white illustrations.

The first and only collection available of molecular methods for tissue engineers. Written by leading researchers who have perfected the methods, the book provides step-by-step procedures for fabricating tissues, tissue equivalents, polymers, scaffolds, isolating cells, and neo-organs. It also provides advanced methods for evaluating forces on cells and tissues, for quantitatively studying these cells and tissue, and for transplantation.

"Jeffrey Morgan and Martin Yarmush bring together in Tissue Engineering Methods and Protocols the first major collection of cutting-edge methods developed and used by leading researchers in the tissue engineering field. These hands-on experts describe easily reproducible protocols for the production and evaluation of polymers, scaffolds, and composites, as well as methods for the isolation, culture, and analysis of cells, including the combination of cells with a variety of materials or devices. Quantitative methods designed to assess cell performance and cell function that will also illuminate our understanding of cellular processes and the cellular response to the chemical and mechanical environment of a functional organ/tissue are also offered."--Jacket Front Matter....Pages i-xvi Front Matter....Pages 1-1 Front Matter....Pages 3-17 Front Matter....Pages 19-33 Front Matter....Pages 35-45 Front Matter....Pages 47-56 Front Matter....Pages 57-65 Front Matter....Pages 67-73 Back Matter....Pages 75-83 ....Pages 85-100 Certain analogs of the extracellular matrix (ECM) have been shown to possess surprising morphogenetic activity during healing of lesions in various anatomical sites.