Therapeutic monoclonal antibodies: from the bench to the clinic

70-chapter authoritative reference that covers therapeutic monoclonal antibody discovery, development, and clinical applications while incorporating principles, experimental data, and methodologies. First book to address the discovery and development of antibody therapeutics in their entirety. Most chapters contain experimental data to illustrate the principles described in them. Authors provide detailed methodologies that readers can take away with them and use in their own laboratories THERAPEUTIC MONOCLONAL ANTIBODIES......Page 5 CONTENTS......Page 7 FOREWORD......Page 11 PREFACE......Page 13 CONTRIBUTORS......Page 15 PART I ANTIBODY BASICS......Page 19 1. Therapeutic Monoclonal Antibodies: Past, Present, and Future......Page 21 2. Antibody Molecular Structure......Page 69 3. Glycosylation of Therapeutic IgGs......Page 85 4. Antibody Databases and Tools: The IMGT(®) Experience......Page 109 PART II ANTIBODY SOURCES......Page 133 5. Human Antibodies from Transgenic Mice......Page 135 6. Rabbit Hybridoma......Page 169 7. Human Antibody Repertoire Libraries......Page 187 PART III IN VITRO DISPLAY TECHNOLOGY......Page 207 8. Antibody Phage Display......Page 209 9. Yeast Surface Display......Page 231 10. Ribosomal Display......Page 257 11. Bacterial Display of Antibodies......Page 273 12. Antibody Selection from Immunoglobulin Libraries Expressed in Mammalian Cells......Page 301 PART IV ANTIBODY ENGINEERING......Page 327 13. Antibody Engineering: Humanization, Affinity Maturation, and Selection Techniques......Page 329 14. Modulation of Serum Protein Homeostasis and Transcytosis by the Neonatal Fc Receptor......Page 353 15. Engineering the Antibody Fc Region for Optimal Effector Function......Page 367 PART V PHYSIOLOGY AND IN VIVO BIOLOGY......Page 389 16. Antibody-Complement Interaction......Page 391 17. Bacteria Immunoglobulin-Binding Proteins: Biology and Practical Applications......Page 403 18. Immunogenicity Screening Using in Silico Methods: Correlation between T-Cell Epitope Content and Clinical Immunogenicity of Monoclonal Antibodies......Page 435 19. Monoclonal Antibody Pharmacokinetics and Pharmacodynamics......Page 457 20. Biodistribution and Imaging......Page 479 21. Antibodies and the Blood-Brain Barrier......Page 501 PART VI ANTIBODY CHARACTERIZATION......Page 521 22. Determination of Equilibrium Dissociation Constants......Page 523 23. Molecular and Functional Characterization of Monoclonal Antibodies......Page 543 24. Characterization of Heterogeneity in Monoclonal Antibody Products......Page 559 PART VII ANTIBODY EXPRESSION......Page 573 25. Antibody Expression in Mammalian Cells......Page 575 26. Production of Antibodies in Pichia pastoris......Page 591 27. Production of Antibody Fab' Fragments in E. coli......Page 607 28. Production of Human Therapeutic Monoclonal Antibodies in Chicken Eggs......Page 641 29. Production of Antibodies in Plants......Page 669 PART VIII THERAPEUTIC ANTIBODIES......Page 691 30. The Formulation and Delivery of Monoclonal Antibodies......Page 693 31. Therapeutic Antibodies in Clinical Use and Leading Clinical Candidates......Page 729 32. Follow-On Protein Products: What, Where, When, How?......Page 781 33. Monomeric Fc Fusion Molecules......Page 797 34. Radioimmunotherapy: Current Status and Future Directions......Page 815 35. Antibody-Drug Conjugate Therapy......Page 839 ABBREVIATIONS......Page 875 INDEX......Page 883

Brings together the state of knowledge and practice in therapeutic monoclonal antibodies

The first therapeutic antibody, murine-derived Muromonab OKT3®, was approved by the FDA in 1986 to treat acute organ rejection. Since then, monoclonal antibody therapeutics have played an increasing role in combating human diseases. To discover the state of the science and where this burgeoning field is heading, readers should turn to this 35-chapter work. It is the first book that draws together all the latest findings, tools, and practices that span the entire process of discovery, development, and clinical application of therapeutic monoclonal antibodies.

Therapeutic Monoclonal Antibodies reviews:

• Basic antibody biology and antibody sources

• Antibody engineering

• Physiology and in vivo biology

• Antibody production and delivery

• Antibody therapeutic targets

• Therapeutic monoclonal antibodies in clinical use and in clinical trial

• Biogenerics/biosimilars

Chapters are written by one or more leading practitioners with hands-on experience in the area. The authors have provided not only theories but also methodologies that readers can use in their own laboratories. Moreover, experimental data is offered in most chapters to illustrate key principles.

Therapeutic Monoclonal Antibodies is recommended for biotechnologists, immunologists, medicinal and pharmaceutical chemists, bioengineers, bioprocess technologists, biochemists, and students. It will help them advance their own basic and clinical research by taking advantage of the latest findings in the discovery, development, and clinical application of therapeutic monoclonal antibodies.

The first therapeutic antibody, murine-derived Muromonab OKT3ʼ, was approved by the FDA in 1986 to treat acute organ rejection. Since then, monoclonal antibody therapeutics have played an increasing role in combating human diseases. To discover the state of the science and where this burgeoning field is heading, readers should turn to this 35-chapter work. It is the first book that draws together all the latest findings, tools, and practices that span the entire process of discovery, development, and clinical application of therapeutic monoclonal antibodies * 70-chapter authoritative reference that covers therapeutic monoclonal antibody discovery, development, and clinical applications while incorporating principles, experimental data, and methodologies. * First book to address the discovery and development of antibody therapeutics in their entirety. * Most chapters contain experimental data to illustrate the principles described in them. * Authors provide detailed methodologies that readers can take away with them and use in their own laboratories