ماهی زبرا، جلد اول: زیست‌شناسی (جلد در مجموعه روش‌ها در زیست‌شناسی سلولی)

This is the first in a two-volume, comprehensive treatment of the methodologies used in researching the Zebrafish, an emerging vertebrate model system. The text includes discussions on development, genetic methodologies, and model applications. Key Features * Details state-of-the-art zebrafish protocols in a single-source reference * Presents methods and reagents in user-friendly format * Delineates critical steps and pitfalls of he procedures * Illustrates techniques with full-color plates * Summarizes many new and interesting developmental mutants * Includes appendices with strain information and a compendium of zebrafish World Wide Web sites * Relevant to clinicians interested in vertebrate models of human congenital diseases Content: Series Editors Page ii Edited by Page iii Copyright Page Page iv Dedication Page v Contributors Pages xiii-xv Preface Pages xvii-xviii H. William Detrich III Chapter 1 Overview of the Zebrafish System Original Research Article Pages 3-10 H. William Detrich III, Monte Westerfield, Leonard I. Zon Chapter 2 Cell Cycles and Development in the Embryonic Zebrafish Original Research Article Pages 11-26 Donald A. Kane Chapter 3 Zebrafish Embryonal Cell Culture Original Research Article Pages 29-37 Angela Helmrich, David Barnes Chapter 4 Primary Fibroblast Cell Culture Original Research Article Pages 39-43 Barry H. Paw, Leonard I. Zon Chapter 5 Production of Haploid and Diploid Androgenetic Zebrafish (Including Methodology for Delayed in Vitro Fertilization) Original Research Article Pages 45-60 Graham E. Corley-Smith, Bruce P. Brandhorst, Charline Walker, John H. Postlethwait Chapter 6 Analysis of Protein and Gene Expression Original Research Article Pages 63-85 Trevor Jowett Chapter 7: Strategies to Perturb Zebrafish Development Original Research Article Pages 87-115 Matthias Hammerschmidt, Patrick Blader, Uwe Strähle Chapter 8 Vectors and Techniques for Ectopic Gene Expression in Zebrafish Original Research Article Pages 117-126 Tana M. Hyatt, Stephen C. Ekker Chapter 9 Analysis of Zebrafish Development Using Explant Culture Assays Original Research Article Pages 127-156 Yevgenya Grinblat, Mary Ellen Lane, Charles Sagerström, Hazel Sive Chapter 10 Embryonic Axis Formation in the Zebrafish Original Research Article Pages 159-178 Mary C. Mullins Chapter 11 Confocal Microscopic Analysis of Morphogenetic Movements Original Research Article Pages 179-204 Mark S. Cooper, Leonard A. D'Amico, Clarissa A. Henry Chapter 12 Cytoskeletal Dynamics of the Zebrafish Embryo Original Research Article Pages 205-226 Jacek Topczewski, Lilianna Solnica-Krezel Chapter 13 Kinesin–like Microtubule Motors in Early Development Original Research Article Pages 227-250 Ming-Chyuan Chen, H. William Detrich III Chapter 14 Techniques in Neural Development Original Research Article Pages 253-272 Cecilia B. Moens, Andreas Fritz Chapter 15 Development of the Retina Original Research Article Pages 273-299 Jarema Malicki Chapter 16 Growth Control in the Ontogenetic and Regenerating Zebrafish Fin Original Research Article Pages 301-311 Stephen L. Johnson, Paul Bennett Chapter 17 Vascular and Blood Gene Expression Original Research Article Pages 313-336 Leon H. Parker, Leonard I. Zon, Didier Y.R. Stainier Chapter 18 Analysis of Hemostasis in the Zebrafish Original Research Article Pages 337-357 Pudur Jagadeeswaran, Yuan C. Liu, John P. Sheehan Chapter 19 Cell Lineage Tracing in Heart Development Original Research Article Pages 359-365 Fabrizio C. Serluca, Mark C. Fishman Chapter 20 Neurogenesis in Zebrafish Embryos Original Research Article Pages 367-386 Ajay B. Chitnis, Igor B. Dawid Index Pages 387-391 The Zebrafish: Biology Presents Laboratory Protocols And Reviews That Focus On Danio Rerio, The Tropical, Freshwater Fish That Has Emerged As An Extremely Powerful Molecular-genetic Model System For Analysis Of Vertebrate Development. This Volume Describes The Embryology Of The Zebrafish, Presents Methods For Cell Culture And For The Study Of Gene Expression And Function In Development, Highlights Cellular Techniques For Analysis Of Morphogenesis, And Concludes With Protocols For The Study Of And Descriptions Of Mutations That Perturb Organogenesis. Accessible To Novices And Old Hands Alike, This Volume Summarizes All Aspects Of Zebrafish Cell And Developmental Biology.--book Jacket. Part I: Introduction. 1. Overview Of The Zebrafish System / H. William Detrich, Iii, Monte Westerfield, And Leonard I. Zon -- 2. Cell Cycles And Development In The Embryonic Zebrafish / Donald A. Kane -- Part Ii: Cell Culture And General Methods. 3. Zebrafish Embryonal Cell Culture / Angela Helmrich And David Barnes -- 4. Primary Fibroblast Cell Culture / Barry H. Paw And Leonard I. Zon -- 5. Production Of Haploid And Diploid Androgenetic Zebrafish (including Methodology For Delayed In Vitro Fertilization) / Graham E. Corley-smith, Et Al. -- Part Iii. Gene Expression And Function In Development. 6. Analysis Of Protein And Gene Expression / Trevor Jowett -- 7. Strategies To Perturb Zebrafish Development / Matthias Hammerschmidt, Patrick Blader, And Uwe Strahle -- 8. Vectors And Techniques For Ectopic Gene Expression In Zebrafish / Tana M. Hyatt And Stephen C. Ekker -- 9. Analysis Of Zebrafish Development Using Explant Culture Assays / Yevgenya Grinblat, Et Al. -- Part Iv. Early Embryonic Development. 10. Embryonic Axis Formation In The Zebrafish / Mary C. Mullins -- 11. Confocal Microscopic Analysis Of Morphogenetic Movements / Mark S. Cooper, Leonard A. D'amico, And Clarissa A. Henry -- 12. Cytoskeletal Dynamics Of The Zebrafish Embryo / Jacek Topczewki And Lilianna Solnica-krezel -- 13. Kinesin-like Microtubule Motors In Early Development / Ming-chyuan Chen And H. William Detrich, Iii -- Part V. Organogenesis. 14. Techniques In Neural Development / Cecilia B. Moens And Andreas Fritz -- 15. Development Of The Retina / Jarema Malicki -- 16. Growth Control In The Ontogenetic And Regenerating Zebrafish Fin / Stephen L. Johnson And Paul Bennett -- 17. Vascular And Blood Gene Expression / Leon H. Parker, Leonard I. Zon, And Didier Y.r. Stainier -- 18. Analysis Of Hemostasis In The Zebrafish / Pudur Jagadeeswaran, Yuan C. Liu, And John P. Sheehan -- 19. Cell Lineage Tracing In Heart Development / Fabrizio C. Serluca And Mark C. Fishman -- 20. Neurogenesis In Zebrafish Embryos / Ajay B. Chitnis And Igor B. Dawid. Edited By H. William Detrich, Iii, Monte Westerfield, Leonard I. Zon. Prepared Under The Auspices Of The American Society For Cell Biology. Includes Bibliographical References And Index. This is the first in a two-volume, comprehensive treatment of the methodologies used in researching the Zebrafish, an emerging vertebrate model system. The text includes discussions on development, genetic methodologies, and model applications.

Key Features
* Details state-of-the-art zebrafish protocols in a single-source reference
* Presents methods and reagents in user-friendly format
* Delineates critical steps and pitfalls of he procedures
* Illustrates techniques with full-color plates
* Summarizes many new and interesting developmental mutants
* Includes appendices with strain information and a compendium of zebrafish World Wide Web sites
* Relevant to clinicians interested in vertebrate models of human congenital diseases