The Peroxidase Multigene Family of Enzymes : Biochemical Basis and Clinical Applications

In September 1998 experts from 19 countries came together for an interdisciplinary discussion of the function of animal peroxidases, a family of enzymes embracing myeloperoxidase, eosinophil peroxidase, thyroid peroxidase and lactoperoxidase. Their papers have been updated for publication, yielding a wide-ranging overview of the state of the art. The chapters cover a wide range of topics, including three-dimensional structure of representative family members, their biosynthesis and intracellular transport, mechanism of action as well as applications to clinical medicine. They are of clinical relevance in, for example, arteriosclerosis, multiple sclerosis, infections, tumorigenesis, rheumatic diseases and hypothyroidism. This book forms an excellent introduction for anyone interested in the peroxidase family of enzymes. Front Matter....Pages I-XII Peroxidases: A Historical Overview of Milestones in Research on Myeloperoxidase....Pages 1-10 Targeting of Proteins to Lysosomes and Granules....Pages 11-21 Sorting and Processing of Neutrophil Granule Proteins....Pages 22-30 X-Ray Crystallographic Studies of Human Myeloperoxidase....Pages 31-37 Structural and Biological Properties of Human Recombinant Myeloperoxidase....Pages 38-44 Recent Insights into the Biosynthesis and Processing of Human Myeloperoxidase....Pages 45-51 Targets for Myeloperoxidase-Dependent Bactericidal Activity....Pages 52-57 Reactions of Myeloperoxidase and Production of Hypochlorous Acid in Neutrophil Phagosomes....Pages 58-67 Acquisition and Use of Myeloperoxidase in the Microbicidal Activity of Macrophages....Pages 68-71 Cooperative Interactions of the Peroxidase and Nitric Oxide Pathways in Inflammatory Oxidant Production....Pages 72-85 The Role of the Monoclonal Antibody Anti-Myeloperoxidase (anti-MPO) in the Diagnosis and Classification of Acute Leukaemias....Pages 86-92 The Role of Myeloperoxidase in Myeloid Leukemia and Multiple Sclerosis....Pages 93-101 Pathways for Oxidative Tissue Injury by Myeloperoxidase....Pages 102-106 Myeloperoxidase Activity of Neutrophils in Cystic Fibrosis....Pages 107-113 Structure Function Relationships Amongst Members of the Animal Peroxidase Family of Proteins....Pages 114-121 Myeloperoxidase (MPO) and Eosinophil Peroxidase (EPO) Deficiency in the Region Friuli-Venezia Giulia of North-Eastern Italy....Pages 122-128 Biochemistry and Physiology of Thyroid Peroxidase....Pages 129-132 Severe Congenital Hypothyroidism Caused by Mutations in the Thyroid Peroxidase Gene....Pages 133-135 Screening for Leukocyte Peroxidase Deficiencies by Means of Flow Cytometry: Application to the Study of Prevalence, Pathology and Genetics....Pages 136-144 Prevalence of Inherited Myeloperoxidase Deficiency in Japan....Pages 145-149 Acquired and Inherited Forms of Myeloperoxidase Deficiency: Clinical and Hematological Features....Pages 150-156 Myeloperoxidase-Positive and Negative Granulocytes: Investigations by Flow Cytometry....Pages 157-165 Mutation Analysis for Genotype-Phenotype Relationships in Myeloperoxidase Deficiency....Pages 166-172 Diagnostic Significance of Antineutrophil Cytoplasmic Antibodies (ANCA) in Systemic Vasculitides....Pages 173-179 Immunodiagnostic Aspects of Autoantibodies Against Myeloperoxidase....Pages 180-185 Pathophysiological Mechanisms in Anti-Myeloperoxidase-Associated Vasculitis....Pages 186-190 Back Matter....Pages 191-193