The Molecular Biology of Adenoviruses 1 : 30 Years of Adenovirus Research 1953-1983

A puzzling epidemiological problem was the driving force behind the discovery of human adenoviruses by Wallace Rowe and his colleagues 30 years ago. The de­ velopment of a plaque assay for poliomyelitis virus in 1953 led us to the threshold of quantitative virology, and in the same year the double-helical structure of DNA was discovered and became a cornerstone of mo­ lecular biology. The potential of adenoviruses as research tools in the molecular and cellular biology of eukaryotic cells was recognized as early as the late 1950s and early 1960s by several investigators. Structural and biochemical stu­ dies dominated the early years. In 1962, some of the adenoviruses were the first human viruses shown to be oncogenic in experimental animals. Thus adenovirology offered the investigator the entire gamut of host cell interactions, productive and abortive, as well as trans­ formed and tumor cell systems. The possibilities that adenoviruses afforded for the study of the molecular biology and genetics of eukaryotic cells were fully rea­ lized in the late 1960s and the 1970s. Front Matter....Pages I-XII Structure and Assembly of Adenoviruses....Pages 1-52 The Mechanism of Adenovirus DNA Replication and the Characterization of Replication Proteins....Pages 53-73 The Origin of Adenovirus DNA Replication....Pages 75-87 A New Mechanism for the Initiation of Replication of Φ 29 and Adenovirus DNA:Priming by the Terminal Protein....Pages 89-106 The Messenger RNAs from the Transforming Region of Human Adenoviruses....Pages 107-123 In Vitro Transcription of Adenovirus Genes....Pages 125-145 Adenovirus Early Gene Regulation and the Adeno–associated Virus Helper Effect....Pages 147-165 Antibodies to Synthetic Peptides Targeted to the Transforming Genes of Human Adenoviruses: An Approach to Understanding Early Viral Gene Function....Pages 167-192 On the Mechanism of Recombination Between Adenoviral and Cellular DNAs: The Structure of Junction Sites....Pages 193-228 Back Matter....Pages 229-234