The immunoassay handbook : theory and applications of ligand binding, ELISA, and related techniques

The fourth edition of __The Immunoassay Handbook__ provides an excellent, thoroughly updated guide to the science, technology and applications of ELISA and other immunoassays, including a wealth of practical advice. It encompasses a wide range of methods and gives an insight into the latest developments and applications in clinical and veterinary practice and in pharmaceutical and life science research. Highly illustrated and clearly written, this award-winning reference work provides an excellent guide to this fast-growing field. Revised and extensively updated, with over 30% new material and 77 chapters, it reveals the underlying common principles and simplifies an abundance of innovation. __The Immunoassay Handbook__ reviews a wide range of topics, now including lateral flow, microsphere multiplex assays, immunohistochemistry, practical ELISA development, assay interferences, pharmaceutical applications, qualitative immunoassays, antibody detection and lab-on-a-chip. This handbook is a must-read for all who use immunoassay as a tool, including clinicians, clinical and veterinary chemists, biochemists, food technologists, environmental scientists, and students and researchers in medicine, immunology and proteomics. It is an essential reference for the immunoassay industry. * Provides a unique mix of theory, practical advice and applications, with numerous examples * Includes explanations of technologies under development and practical insider tips that are sometimes omitted from scientific papers * Includes a comprehensive troubleshooting guide, useful for solving problems and improving assay performance Content: Front Matter , Page iii Copyright , Page iv Foreword , Pages ix-x , Rosalyn Yalow Preface , Pages xi-xiii , David Wild Acknowledgements , Page xv , David Wild List of Contributors , Pages xvii-xix , Marcos Alcocer, Zane D. Amenhotep, Philip R. Andersen, David Armbruster, John Backus, Steven R. Binder, Ronald R. Bowsher, Catherine A. Brennan, Carey-Ann D. Burnham, Daniel W. Chan, Richard S. Chapman, Nic D. Christofides, Penny M. Clark, Alastair H. Dent, John L. Dessi, Sushil G. Devare, Christopher Doern, Gordon Duffin, David C. Duffy, Sherry A. Dunbar, et al. About the Editors , Page xxi Chapter 1.1 - How to Use This Book , Pages 3-5 , David Wild Chapter 1.2 - Immunoassay for Beginners , Pages 7-10 , David Wild Chapter 1.3 - Immunoassay Performance Measures , Pages 11-26 , Chris Davies Chapter 2.1 - Principles of Competitive and Immunometric Assays (Including ELISA) 1 , Pages 29-59 , Chris Davies Chapter 2.2 - Non-competitive Immunoassays for Small Molecules—the Anti-complex and Selective Antibody Systems , Pages 61-65 , Colin H. Self, Stephen Thompson, Theresa Street, Kelly J. Lamb, Gordon Duffin, John L. Dessi, Maggie Turnbull Chapter 2.3 - Homogeneous Immunoassays , Pages 67-87 , Edwin F. Ullman Chapter 2.4 - Lateral Flow Immunoassay Systems: Evolution from the Current State of the Art to the Next Generation of Highly Sensitive, Quantitative Rapid Assays , Pages 89-107 , Brendan O’Farrell Chapter 2.5 - Ambient Analyte Assay , Pages 109-121 , Roger Ekins Chapter 2.6 - Free Analyte Immunoassay , Pages 123-137 , Nic D. Christofides Chapter 2.7 - Qualitative Immunoassay—Features and Design , Pages 139-147 , Jianwen He, Simon Parker Chapter 2.8 - Detection of Antibodies Relevant to Infectious Disease , Pages 149-155 , Steven Binder, Jennifer A. Isler Chapter 2.9 - Microsphere-Based Multiplex Immunoassays: Development and Applications Using Luminex ® xMAP ® Technology , Pages 157-174 , Sherry A. Dunbar, Michaela R. Hoffmeyer Chapter 2.10 - Lab-on-a-Chip, Micro- and Nanoscale Immunoassay Systems, and Microarrays , Pages 175-202 , Simon Rattle, Oliver Hofmann, Christopher P. Price, Larry J. Kricka, David Wild Chapter 2.11 - Immunological Biosensors , Pages 203-207 , Jason Reed, James K. Gimzewski Chapter 2.12 - Surface Plasmon Resonance in Binding Site, Kinetic, and Concentration Analyses , Pages 209-221 , Robert Karlsson Chapter 2.13 - Measurement of Single Protein Molecules Using Digital ELISA , Pages 223-242 , David M. Rissin, David H. Wilson, David C. Duffy Chapter 3.1 - Antibodies , Pages 245-265 , Eryl Liddell Chapter 3.2 - Signal Generation and Detection Systems (Excluding Homogeneous Assays) , Pages 267-285 , Ian Weeks, Larry J. Kricka, David Wild Chapter 3.3 - Separation Systems , Pages 287-299 , David Wild, Wlad Kusnezow Chapter 3.4 - Conjugation Methods , Pages 301-313 , Alastair H. Dent Chapter 3.5 - Standardization and Calibration , Pages 315-322 , David Wild, Chris Sheehan Chapter 3.6 - Calibration Curve Fitting , Pages 323-336 , John Dunn, David Wild Chapter 4.1 - The Foundations of Immunochemistry , Pages 339-356 , Robert F. Ritchie Chapter 4.2 - Immunohistochemistry and Immunocytochemistry , Pages 357-377 , Simon Renshaw Chapter 5.1 - Practical Guide to ELISA Development , Pages 381-393 , Jianwen He Chapter 5.2 - Method Evaluation—A Practical Guide , Pages 395-402 , Chris Sheehan, Jianwen He, Mari Smith Chapter 5.3 - Interferences in Immunoassay , Pages 403-416 , Jason Y. Park, Larry J. Kricka Chapter 5.4 - Immunoassay Development in the In Vitro Diagnostic Industry , Pages 417-423 , Doug Brandt, Steve Figard Chapter 6.1 - Sample Collection, Including Participant Preparation and Sample Handling , Pages 427-440 , Colin Wilde, Dorothée Out, Sara Johnson, Douglas A. Granger Chapter 6.2 - Quality Assurance , Pages 441-454 , Catharine M. Sturgeon Chapter 6.3 - Point-of-Care Testing , Pages 455-463 , James H. Nichols Chapter 6.4 - Choosing an Automated Immunoassay System , Pages 465-468 , Lori J. Sokoll, Daniel W. Chan Chapter 6.5 - Immunoassay Troubleshooting Guide , Pages 469-506 , David Wild, Jianwen He Chapter 7.1 - Introduction to Immunoassay Product Technology in Clinical Diagnostic Testing , Pages 509-515 , David Wild, Chris Sheehan, Steve Binder Chapter 7.2 - Market Trends , Pages 517-531 , David Huckle Chapter 7.3 - Lateral Flow and Consumer Diagnostics , Pages 533-536 , Sarah Tiplady Chapter 7.4 - Consumer Diagnostics—Clearblue Pregnancy and Fertility Testing , Pages 537-540 , Sarah Tiplady Chapter 7.5 - The Triage ® System , Pages 541-544 , Thomas I. Koshy, Kenneth F. Buechler Chapter 7.6 - Stratus ® CS Acute CareTM STAT Fluorometric Analyzer , Pages 545-547 , Amy Sarli, W.N. McLellan Chapter 7.7 - IMx ® 1 , Pages 549-554 , Kent Ford Chapter 7.8 - AxSYM ® , Pages 555-559 , Theresa Donahoe Chapter 7.9 - Abbott ARCHITECT ® Family of Analyzers , Pages 561-566 , Frank A. Quinn, David A. Armbruster Chapter 7.10 - ADVIA Centaur ® XP , Pages 567-570 , Jean Metzar Chapter 7.11 - IMMULITE ® and IMMULITE 1000 , Pages 571-574 , Arthur L. Babson Chapter 7.12 - IMMULITE ® 2000 and IMMULITE 2000 XPi , Pages 575-578 , Arthur L. Babson Chapter 7.13 - VITROS ® ECiQ Immunodiagnostic System, VITROS ® 3600 Immunodiagnostic System, and VITROS ® 5600 Integrated System , Pages 579-584 , John W. Backus, David Wild Chapter 7.14 - VITROS ® Immuno-Rate and MicroTipTM Assays , Pages 585-588 , John W. Backus, Susan J. Danielson, David A. Hilborn Chapter 7.15 - Access 2 , Pages 589-596 , Katherine M. Leith Chapter 7.16 - DxI 600 and DxI 800 , Pages 597-601 , Katherine M. Leith Chapter 7.17 - Elecsys ® Immunoassay Systems , Pages 603-607 , Mary Beth Myers Chapter 7.18 - PRISMTM , Pages 609-615 , Dinesh Shah, Jim Stewart Chapter 7.19 - Phadia Laboratory Systems , Pages 617-619 , Angela Gore, Gareth Evans, Mats Rilvën Chapter 8.1 - Immunoassay Applications in Veterinary Diagnostics , Pages 623-645 , Thomas P. O’Connor Jr., John Lawrence, Philip Andersen, Valerie Leathers, Erwin Workman Chapter 8.2 - Ligand Binding Assays in Drug Development , Pages 647-657 , Jeffrey M. Sailstad, Ronald R. Bowsher, Omar F. Laterza, William Nowatzke Chapter 9.1 - Clinical Concepts , Pages 661-672 , Chris Davies Chapter 9.2 - Thyroid , Pages 673-693 , Rhys John, Nic Christofides, Carole A. Spencer, David Wild Chapter 9.3 - The Adrenal Cortex , Pages 695-703 , Les Perry, Sami Medbak Chapter 9.4 - Bone and Calcium Metabolism , Pages 705-720 , William D. Fraser, Kay W. Colston, John C. Stevenson Chapter 9.5 - Infertility , Pages 721-734 , Michael J. Wheeler Chapter 9.6 - In Vitro Fertilization and Embryo Transfer (IVF-ET) , Pages 735-743 , Richard Fleming, Sherry Faye Chapter 9.7 - Hirsutism and Virilization in the Female , Pages 745-756 , Michael J. Wheeler Chapter 9.8 - Pregnancy , Pages 757-776 , Kevin Spencer, Tim Chard Chapter 9.9 - Growth and Growth Hormone Deficiency , Pages 777-781 , Gill Rumsby, Peter Hindmarsh, Michael Preece, Jane Pringle Chapter 9.10 - Diabetes Mellitus , Pages 783-794 , Penny Clark, Tim McDonald Chapter 9.11 - Hematology , Pages 795-815 , Katy Evans, Zane Amenhotep, Derek Dawson, Harry Waters, John Ardern Chapter 9.12 - Cardiac Markers , Pages 817-831 , Deborah French, Alan H.B. Wu Chapter 9.13 - Cancer Markers , Pages 833-856 , Hoon H. Sunwoo, Mavanur R. Suresh Chapter 9.14 - Allergy , Pages 857-867 , Marcos Alcocer, Lars Yman Chapter 9.15 - Autoimmune Disease , Pages 869-890 , David F. Keren Chapter 9.16 - Gastrointestinal Tract , Pages 891-900 , Arne Røseth, Richard Chapman, Radra Ramachandran, Chris Sheehan Chapter 9.17 - Hepatitis , Pages 901-911 , Ravi Kaul Chapter 9.18 - HIV-1 and HIV-2: Causative Agents of AIDS , Pages 913-917 , Catherine A. Brennan, Sushil G. Devare Chapter 9.19 - Viral Diseases 1 , Pages 919-927 , Carey-Ann D. Burnham, Christopher Doern, Steven R. Binder Chapter 9.20 - Bacterial Diseases 1 , Pages 929-938 , Carey-Ann D. Burnham, Christopher Doern, Steven R. Binder Chapter 9.21 - Parasites and Fungi , Pages 939-943 , Steven R. Binder, Patrice D. Sarfati Chapter 9.22 - Therapeutic Drug Monitoring (TDM) , Pages 945-962 , Philip A. Routledge, Alun D. Hutchings Chapter 9.23 - Drugs of Abuse , Pages 963-987 , Alun D. Hutchings, Brian Widdop Index , Pages 989-1013

The fourth edition of The Immunoassay Handbook provides an excellent, thoroughly updated guide to the science, technology and applications of ELISA and other immunoassays, including a wealth of practical advice. It encompasses a wide range of methods and gives an insight into the latest developments and applications in clinical and veterinary practice and in pharmaceutical and life science research.

Highly illustrated and clearly written, this award-winning reference work provides an excellent guide to this fast-growing field. Revised and extensively updated, with over 30% new material and 77 chapters, it reveals the underlying common principles and simplifies an abundance of innovation.

The Immunoassay Handbook reviews a wide range of topics, now including lateral flow, microsphere multiplex assays, immunohistochemistry, practical ELISA development, assay interferences, pharmaceutical applications, qualitative immunoassays, antibody detection and lab-on-a-chip.

This handbook is a must-read for all who use immunoassay as a tool, including clinicians, clinical and veterinary chemists, biochemists, food technologists, environmental scientists, and students and researchers in medicine, immunology and proteomics. It is an essential reference for the immunoassay industry.

David Wild is a healthcare industry veteran, with experience in biotechnology, pharmaceuticals, medical devices and immunodiagnostics, which remains his passion. He worked for Amersham, Eastman-Kodak, Johnson & Johnson, and Bristol-Myers Squibb, and consulted for diagnostics and biotechnology companies. He led research and development programs, design and construction of chemical and biotechnology plants, and integration of acquired companies. Director-level positions included Research and Development, Design Engineering, Operations and Strategy, for billion dollar businesses. He retired from full-time work in 2012 to focus on his role as Editor of The Immunoassay Handbook, and advises on product development, manufacturing and marketing.

• Provides a unique mix of theory, practical advice and applications, with numerous examples
• Includes explanations of technologies under development and practical insider tips that are sometimes omitted from scientific papers
• Includes a comprehensive troubleshooting guide, useful for solving problems and improving assay performance

This book is an overview of the strategies to generate high-quality films of one-dimensional semiconductor nanostructures on flexible substrates (e.g., plastics) and the use of them as building blocks to fabricating flexible devices (including electronics, optoelectronics, sensors, power systems). In addition to engineering aspects, the physics and chemistry behind the fabrication and device operation will also be discussed as well. Internationally recognized scientists from academia, national laboratories, and industries, who are the leading researchers in the emerging areas, are contributing exceptional chapters according to their cutting-edge research results and expertise. This book will be an on-time addition to the literature in nanoscience and engineering. It will be suitable for graduate students and researchers as a useful reference to stimulate their research interest as well as facilitate their research in nanoscience and engineering.

• Considers the physics and chemistry behind fabrication and device operation
• Discusses applications to electronics, optoelectronics, sensors and power systems
• Examines existing technologies and investigates emerging trends

This book is an overview of the strategies to generate high-quality films of one-dimensional semiconductor nanostructures on flexible substrates (e.g., plastics) and the use of them as building blocks to fabricating flexible devices (including electronics, optoelectronics, sensors, power systems). In addition to engineering aspects, the physics and chemistry behind the fabrication and device operation will also be discussed as well. Internationally recognized scientists from academia, national laboratories, and industries, who are the leading researchers in the emerging areas, are contributing exceptional chapters according to their cutting-edge research results and expertise. This book will be an on-time addition to the literature in nanoscience and engineering. It will be suitable for graduate students and researchers as a useful reference to stimulate their research interest as well as facilitate their research in nanoscience and engineering. Considers the physics and chemistry behind fabrication and device operation Discusses applications to electronics, optoelectronics, sensors and power systems Examines existing technologies and investigates emerging trends "This book is an overview of the strategies to generate high-quality films of one-dimensional semiconductor nanostructures on flexible substrates (e.g., plastics) and the use of them as building blocks to fabricating flexible devices (including electronics, optoelectronics, sensors, power systems). In addition to engineering aspects, the physics and chemistry behind the fabrication and device operation will also be discussed as well. Internationally recognized scientists from academia, national laboratories, and industries, who are the leading researchers in the emerging areas, are contributing exceptional chapters according to their cutting-edge research results and expertise. This book will be an on-time addition to the literature in nanoscience and engineering. It will be suitable for graduate students and researchers as a useful reference to stimulate their research interest as well as facilitate their research in nanoscience and engineering"--Résumé de l'éditeur "This book is an overview of the strategies to generate high-quality films of one-dimensional semiconductor nanostructures on flexible substrates (e.g., plastics) and the use of them as building blocks to fabricating flexible devices (including electronics, optoelectronics, sensors, power systems). In addition to engineering aspects, the physics and chemistry behind the fabrication and device operation will also be discussed as well. Internationally recognized scientists from academia, national laboratories, and industries, who are the leading researchers in the emerging areas, are contributing exceptional chapters according to their cutting-edge research results and expertise. This book will be an on-time addition to the literature in nanoscience and engineering. It will be suitable for graduate students and researchers as a useful reference to stimulate their research interest as well as facilitate their research in nanoscience and engineering"--Publisher The 4e of this award-winning reference work provides an excellent, thoroughly updated guide to the science, technology and applications of ELISA and other immunoassays, including a wealth of practical advice. It encompasses a wide range of methods and gives an insight into the latest developments and applications in clinical and veterinary practice and in pharmaceutical and life science research. Highly illustrated and clearly written, The Immunoassay Handbook provides an excellent guide to this fast-growing field. Revised and extensively updated, with over 30% new material and 77 ch.