The Cell Cycle: Regulators, Targets, and Clinical Applications (Gwumc Department of Biochemistry and Molecular Biology Annual Spring Symposia)

Interest in the cell cycle has grown explosively in recent years as a result of the identification of key cell cycle regulators and their substrates. Aside from enhancing our understanding of normal cellular growth controls, this new knowledge has also becn valuable in elucidating mechanisms of growth deregulation which occur in diseased states, such as cancer and, in some instances, viral or parasitic infections. The Thirteenth Washington International Spring Symposium was organized with the intention of bringing together scientists working on different aspects of the cell cycle. Scientific topics presented ranged from molecular regulators and effectors to mitosis­ specific changes in cell architecture to the role of the cell cycle in development and disease. The goal of this gathering was to help formulate a more comprehensive and integrated picture of events driving and being driven by the cell cycle, as well as to evaluate the possibilities for clinical application of this knowledge. This symposium, held in Washington, D.C. from May 10-14, 1993, was attended by more than 400 scientists from 20 countries, including many of the scientific leaders in this field. This volume contains most of the papers presented at the seven plenary sessions in addition to selected contributions from a total of nine special oral and poster sessions. Front Matter....Pages i-xi Front Matter....Pages 1-1 Protein Phosphorylation and the Regulation of Key Events in Oocyte and Egg Cell Cycles....Pages 3-15 Control of G1 Progression by Mammalian D-Type Cyclins....Pages 17-23 Phosphorylation in the Regulation of Protein Phosphatases....Pages 25-32 Positive and Negative Regulation of Cell Cycle Progression by Serine/Threonine Protein Phosphatases....Pages 33-40 Effects of Phosphatase Inhibitors on Mammalian p34 cdc2 Kinase Activities....Pages 41-49 The Meiotic Role of twine , A Drosophila Homologue of cdc25 ....Pages 51-57 Front Matter....Pages 59-59 Extracellular Signal-Regulated Protein Kinases (ERKS) 1, 2, and 3....Pages 61-66 Cell Cycle Traverse and Growth Arrest Control in Senescent Human Fibroblasts....Pages 67-78 Cellular Senescence and the Cell Cycle....Pages 79-89 Cell Cycle Targets of Viral Oncoproteins....Pages 91-97 A GTPase Cycle Coupled to the Cell Cycle....Pages 99-110 Sphingolipids Metabolites: A New Class of Second Messengers in the Regulation of Cell Growth....Pages 111-119 Front Matter....Pages 121-121 Histone Gene Transcription During the Cell Cycle....Pages 123-125 G1-S Regulatory Promoter Elements and their Interacting Transcription Factors....Pages 127-139 Regulation of Thymidylate Synthase Gene Expression in Growth-Stimulated Cells....Pages 141-147 The Role of the Transcription Factor E2F in the Growth Regulation of DHFR....Pages 149-154 Activation of the Heat Shock Transcription Factor During G 1 ....Pages 155-161 DNA Damage and Cell Cycle Regulation in S. Cerevisiae ....Pages 163-166 Preferential Repair of Cisplatin Adducts in the Human DHFR Gene During G 1 Phase Assayed with T4 DNA Polymerase....Pages 167-171 Front Matter....Pages 173-173 Direct Inhibition of p107 WEE1 by the Nim 1/CDR 1 Kinase....Pages 175-184 Front Matter....Pages 173-173 A Human Phosphotyrosine Phosphatase Associated with M Phase-Promoting Factor....Pages 185-188 The Localisation of Human Cyclins and CDKS in the Cell Cycle....Pages 189-195 Regulation of Nuclear Envelope Assembly and Disassembly by ARF and other GTP-Binding Proteins....Pages 197-202 Possible Role of the Multi Catalytic Proteinase (Proteasome) in Regulating of the Cell Cycle....Pages 203-209 Cytostellin: A Nuclear Protein that Redistributes to Peripheral Cytoskeletal Locations During Mitosis and G1....Pages 211-221 Evidence for M-Phase-Specific Modification of a Gap Junction Protein....Pages 223-228 Analysis of Centrosome Replication Events in Mammalian Cells....Pages 229-235 Requirements for Microtubule Polymerization and a Calcium Surge for the Metaphase-to-Interphase Transition in Mature Mouse Oocytes....Pages 237-243 Front Matter....Pages 245-245 Mos Proto-Oncogene and Cell Cycle Regulation....Pages 247-249 Altered Regulation of Cell Cycle Genes and Proteins in Senescent Human Diploid Fibroblasts....Pages 251-262 Cell Proliferation as a Biomarker of Age and Development....Pages 263-269 Heat Shock Genes and Cell Cycle Regulation During Early Mammalian Development....Pages 271-281 Expression of G1 Cyclins During Early Development of Zebrafish Embryos....Pages 283-289 Apoptosis: Definition, Roles and Regulation....Pages 291-299 Front Matter....Pages 301-301 Molecules of Deregulated Cell Cycle Control in Cancer....Pages 303-309 The Two Amino Terminal Transforming Functions of the SV40 Large T-Antigen are Required to Overcome P53 Mediated Growth Arrest....Pages 311-317 Down Regulation of Candidate Tumor Suppressor Genes in Breast Cancer....Pages 319-322 Expression and Regulation of Cyclin Genes in Breast Cancer Cells....Pages 323-329 Genotoxin-Induced Apoptosis: Implications for Carcinogenesis....Pages 331-340 Monitoring and Repair of DNA Damage During G 2 in Relation To Carcinogenesis....Pages 341-346 Front Matter....Pages 301-301 Cell Cycle Regulation in Normal Versus Leukemic T Cells....Pages 347-357 Cells Undergoing HIV Envelope-Mediated Programmed Degeneration Accumulate in G2/M Phase....Pages 359-366 Front Matter....Pages 367-367 The Involvement of the Cell Cycle in Apoptosis....Pages 369-378 Cell Cycle Regulation and the Chemosensitivity of Cancer Cells....Pages 379-388 Multiple Cell Cycle Checkpoint Override and its Potential for Binary Tumor Therapy....Pages 389-396 Radiation Induced G2 Delay and Mitotic Cyclin Expression....Pages 397-403 Cyclin B Degradation as a Target of Antiproliferative Drug Action....Pages 405-410 Clinical Relevance of DNA Ploidy and Cell Cycle Phases in Transitional Cell Carcinoma of the Renal Pelvis and Ureter: A Study by Means of Static DNA-Cytophotometry....Pages 411-419 Back Matter....Pages 421-430