The Biology of DNA Tumor Viruses : With an Introduction by Renato Dulbecco

Milestones in Current Research is a series of reprint collections dis­ tinguished from other such publications by new concepts in preparation, presentation, and intent. The aim of each volume is to gather for a given field the seminal contributions that have defined and shaped the trends within the most active areas of current research. The selections for each volume and the structure of the book have been determined with the help of a novel tech­ nique of bibliographic analysis and have then been presented to an acknowl­ edged scientific authority for minor adjustments and the writing of an In­ troduction. These introductions will lend historic perspective to the material selected for each volume. The bibliographic analysis used tends to select papers central to the areas of current research within, roughly, the last decade and is a systematic procedure for depicting, delineating, and covering all such areas over a wide spectrum of scientific research. It is hoped that with this procedure it will be possible to achieve an objectivity, authority, and thoroughness not reached by others and that the timeliness of the volumes will not be limited to just a few years. Each volume should have the permanent value of a historical statement and yet be suf­ ficiently interesting to active researchers in the field as well as to students exploring the quiet way in which the relentless drama of research unfolds in the journal literature. Front Matter....Pages i-xi Front Matter....Pages 1-1 Introduction....Pages 3-10 Front Matter....Pages 11-11 Evidence for a Ring Structure of Polyoma Virus DNA....Pages 13-20 The Cyclic Helix and Cyclic Coil Forms of Polyoma Viral DNA....Pages 21-29 The Twisted Circular Form of Polyoma Viral DNA....Pages 31-38 A Dye-Buoyant-Density Method for the Detection and Isolation of Closed Circular Duplex DNA: The Closed Circular DNA in HeLa Cells....Pages 39-46 Front Matter....Pages 47-47 Viral DNA in Polyoma- and SV40-Transformed Cell Lines....Pages 49-56 The Integrated State of Viral DNA in SV40-Transformed Cells....Pages 57-64 Front Matter....Pages 65-65 A Specific Complement-Fixing Antigen Present in SV40 Tumor and Transformed Cells....Pages 67-75 Detection of Specific Antigen in SV40-Transformed Cells by Immunofluorescence....Pages 77-84 Synthesis of SV40 Tumor Antigen during Replication of Simian Papovavirus (SV40)....Pages 85-88 Front Matter....Pages 89-89 Studies on the Transfer of Subviral Infectivity from SV40-Induced Hamster Tumor Cells to Indicator Cells....Pages 91-99 Activation of Production of Infectious Tumor Virus SV40 in Heterokaryon Cultures....Pages 101-107 Production of SV40 Virus in Heterokaryons of Transformed and Susceptible Cells....Pages 109-116 Front Matter....Pages 117-117 Virus-Specific RNA in Cells Productively Infected or Transformed by Polyoma Virus....Pages 119-133 Characterization of the Simian Virus 40 — specific RNA in Virus-yielding and Transformed Cells....Pages 135-149 Regulation of Transcription of the SV40 DNA in Productively Infected and in Transformed Cells....Pages 151-158 The Transcription of the SV40 Genome in Productively Infected and Transformed Cells....Pages 159-166 Strand Orientation of Simian Virus 40 Transcription in Productively Infected Cells....Pages 167-176 A Map of Simian Virus 40 Transcription Sites Expressed in Productively Infected Cells....Pages 177-189 Presence of Cell and Virus Specific Sequences in the Same Molecules of Nuclear RNA from Virus Transformed Cells....Pages 191-199 Front Matter....Pages 201-201 Induction of Cellular DNA Synthesis by Polyoma Virus....Pages 203-210 Abortive Transformation by Polyoma Virus....Pages 211-224 Cell-Transforming Ability of a Temperature-Sensitive Mutant of Polyoma Virus....Pages 225-230 Abortive Transformation by the Tsa Mutant of Polyoma Virus....Pages 231-233 Temperature-Dependent Properties of Cells Transformed by a Thermosensitive Mutant of Polyoma Virus....Pages 235-241 Front Matter....Pages 243-243 Growth Control in Cultured Cells: Selection of Sublines with Increased Sensitivity to Contact Inhibition and Decreased Tumor-Producing Ability....Pages 245-252 Mutation Causing Temperature-Sensitive Expression of Cell Transformation by a Tumor Virus....Pages 253-268 Back Matter....Pages 269-273