Techniques In Protein Chemistry Vi Techniques In Protein Chemistry 6

TECHNIQUES IN PROTEIN CHEMISTRY VI, an invaluable bench-top reference source for protein chemists, highlights current methods in the following areas: Protein sequencing and amino acid analysis Mass spectral analysis of peptides and proteins Posttranslational processing High-sensitivity protein and peptide separations Protein folding and NMR Analysis of protein interactions Protein design and engineering Techniques in Protein Chemistry VI , an invaluable bench-top reference source for protein chemists, highlights current methods in the following areas: * Protein sequencing and amino acid analysis * Mass spectral analysis of peptides and proteins * Posttranslational processing * High-sensitivity protein and peptide separations * Protein folding and NMR * Analysis of protein interactions * Protein design and engineering Content: Foreword Page xv Joseph J. Villafranca Preface Page xvii John W. Crabb Acknowledgments Page xix The use of a volatile N-terminal degradation reagent for rapid, high-sensitivity sequence analysis of peptides by generation of sequence ladders Original Research Article Pages 3-11 M. Bartlet-Jones, W.A. Jeffery, H.F. Hansen, D.J.C. Pappin Investigation of polyethylene membranes as potential sample support for linking SDS-PAGE with MALDI-TOF MS for the mass measurement of proteins Original Research Article Pages 13-19 James A. Blackledge, Anthony J. Alexander Comparison of ESI-MS, LSIMS and MALDI-TOF-MS for the primary structure analysis of a monoclonal antibody Original Research Article Pages 21-30 Leticia Cano, Kristine M. Swiderek, John E. Shively MS based scanning methodologies applied to Conus venom Original Research Article Pages 31-38 A. Grey Craig, Wolfgang H. Fischer, Jean E. Rivier, J. Michael McIntosh, William R. Gray Direct coupling of an automated 2-dimensional microcolumn affinity chromatography-capillary HPLC system with mass spectrometry for biomolecule analysis Original Research Article Pages 39-46 D.B. Kassel, T.G. Consler, M. Shalaby, P. Sekhri, N. Gordon, T. Nadler Edman degradation and MALDI sequencing enables N- and C-terminal sequence analysis of peptides Original Research Article Pages 47-54 Roland Kellner, Gert Talbo, Tony Houthaeve, Matthias Mann Identification of the amino terminal peptide of N-terminally blocked proteins by differential deutero-acetylation using LC/MS techniques Original Research Article Pages 55-62 Craig D. Thulin, Kenneth A. Walsh HPAEC-PAD analysis of monoclonal antibody glycosylation Original Research Article Pages 65-73 Jeffrey Rohrer, Jim Thayer, Nebojsa Avdalovic, Michael Weitzhandler Carbohydrate structure characterization of two soluble forms of a ligand for the ECK receptor tyrosine kinase Original Research Article Pages 75-82 Christi L. Clogston, Patricia L. Derby, Robert Toso, James D. Skrine, Ming Zhang, Vann Parker, G. Michael Fox, Timothy D. Bartley, Hsieng S. Lu Characterisation of individual N -and O -linked glycosylation sites using Edman degradation Original Research Article Pages 83-90 A.A. Gooley, N.H. Packer, A. Pisano, J.W. Redmond, K.L. Williams, A. Jones, M. Loughnan, P.F. Alewood The unexpected presence of hydroxylysine in non-collagenous proteins Original Research Article Pages 91-98 Michael S. Molony, Shiaw-Lin Wu, Lene K. Keyt, Reed J. Harris Isolation of Escherichia coli synthesized recombinant proteins that contain ε-N-acetyllysine Original Research Article Pages 99-106 Bernard N. Violand, Michael R. Schlittler, Cory Q. Lawson, James F. Kane, Ned R. Siegel, Christine E. Smith, Kevin L. Duffin LC-MS methods for selective detection of posttranslational modifications in proteins: Glycosylation, phosphorylation, sulfation, and acylation Original Research Article Pages 107-116 Mark F. Bean, Roland S. Annan, Mark E. Hemling, Mary Mentzer, Michael J. Huddleston, Steven A. Carr Identification of phosphorylation sites by Edman degradation Original Research Article Pages 117-123 John D. Shannon, Jay W. Fox Determination of the disulfide bonds of human macrophage chemoattractant protein-1 using a gas phase sequencer Original Research Article Pages 125-132 Ramnath Seetharam, Jeanne I. Corman, Shubhada M. Kamerkar Enzymatic digestion of PVDF-bound proteins in the presence of glucopyranoside detergents: Applicability to mass spectrometry Original Research Article Pages 135-142 Joseph Fernandez, Farzin Gharahdaghi, Sheenah M. Mische In gel digestion of SDS PAGE-Separated proteins: Observations from internal sequencing of 25 proteins Original Research Article Pages 143-152 Kenneth R. Williams, Kathryn L. Stone Peptide mapping at the 1 μg level: In-gel vs. PVDF digestion techniques Original Research Article Pages 153-160 Lee Anne Merewether, Christi L. Clogston, Scott D. Patterson, Hsieng S. Lu Enzymatic digestion of proteins in zinc chloride and ponceau s stained gels Original Research Article Pages 161-167 Sharleen Zhou, Arie Admon Direct collection onto Zitex and PVDF for Edman sequencing: Elimination of polybrene Original Research Article Pages 169-176 William A. Burkhart, Mary B. Moyer, Wanda M. Bodnar, Anita M. Everson, Violeta G. Valladares, Jerome M. Bailey Minimizing N-to-O shift in Edman sequencing Original Research Article Pages 177-184 William H. Vensel, George E. Tarr The hydrolysis process and the quality of amino acid analysis: ABRF-94AAA collaborative trial Original Research Article Pages 185-192 K. Ümit Yüksel, Thomas T. Andersen, Izydor Apostol, Jay W. Fox, Raymond J. Paxton, Daniel J. Strydom A new reagent for cleaving at cystine residues Original Research Article Pages 193-199 C. Mitchell, L. Hinman, L. Miller, P.C. Andrews Protein sequence analysis using microbore PTH separations Original Research Article Pages 201-208 Michael F. Rohde, Christi Clogston, Lee Anne Merewether, Patricia Derby, Kerry D. Nugent Assignment of cysteine and tryptophan residues during protein sequencing: Results of ABRF-94SEQ Original Research Article Pages 209-217 Jay Gambee, Philip C. Andrews, Karen DeJongh, Greg Grant, Barbara Merrill, Sheenah Mische, John Rush Automated C-terminal protein sequence analysis using the hewlett-packard G1009A C-terminal protein sequencing system Original Research Article Pages 219-227 Chad G. Miller, David H. Hawke, Jacqueline Tso, Sherrell Early Applications using an alkylation method for Carboxy-terminal protein sequencing Original Research Article Pages 229-237 MeriLisa Bozzini, Jindong Zhao, Pau-Miau Yuan, Doreen Ciolek, Yu-Ching Pan, John Horton, Daniel R. Marshak, Victoria L. Boyd C-terminal sequence analysis of polypeptides containing C-terminal proline Original Research Article Pages 239-247 Jerome M. Bailey, Oanh Tu, Gilbert Issai, John E. Shively High sensitivity detection of tryptic digests using derivatization and fluorescence detection Original Research Article Pages 251-258 Steven A. Cohen, Igor Mechnikov, Patricia Young Reagents for rapid reduction of disulfide bonds in proteins Original Research Article Pages 259-266 Rajeeva Singh, George M. Whitesides Strategies for the removal of ionic and non-ionic detergents from protein and peptide mixtures for on- and off-line liquid chromatography mass spectrometry (LCMS) Original Research Article Pages 267-275 Kristine M. Swiderek, Michael L. Klein, Stanley A. Hefta, John E. Shively Online preparation of complex biological samples prior To analysis by HPLC, LC/MS and/or protein sequencing Original Research Article Pages 277-284 Ken Stoney, Kerry Nugent Methods for the purification and characterization of calcium-binding proteins from retina Original Research Article Pages 285-292 Arthur S. Polans, Krzysztof Palczewski, Wojciech A. Gorczyca, John W. Crabb Evidence for the presence of α-bungarotoxin in venom-derived κ-bungarotoxin Original Research Article Pages 293-299 James J. Fiordalisi, Gregory A. Grant Progress in the development of solvent and chromatography systems appropriate for bitopic membrane proteins Original Research Article Pages 301-310 Song-Jae Kil, Lisa M. Oleksa, Geoffrey C. Landis, Charles R. Sanders II Rapid separation of proteins and peptides using conventional silica-based supports: Identification of 2-D gel proteins following in-gel proteolysis Original Research Article Pages 311-319 Robert L. Moritz, James Eddes, Hong Ji, Gavin E. Reid, Richard J. Simpson Studying α-helix and β-sheet formation in small proteins Original Research Article Pages 323-332 Catherine K. Smith, Mary Munson, Lynne Regan Circular permutation of RNase T1 through PCR based site-directed mutagenesis Original Research Article Pages 333-340 Jane M. Kuo, Leisha S. Mullins, James B. Garrett, Frank M. Raushel E. coli -expressed human neurotrophin-3 characterization of a C-terminal extended product Original Research Article Pages 341-348 John O. Hui, Shi-Yuan Meng, Vishwanatham Katta, Larry Tsai, Michael F. Rohde, Mitsuru Haniu Spectral enhancement of recombinant proteins with tryptophan analogs: The soluble domain of human tissue factor Original Research Article Pages 349-356 C.A. Hasselbacher, R. Rusinova, E. Rusinova, J.B. Alexander Ross Utilization of partial reactions, side reactions, and chemical rescue to analyze site-directed mutants of ribulose 1,5-bisphosphate (RuBP) carboxylase/oxygenase (rubisco) Original Research Article Pages 357-364 Mark R. Harpel, Engin H. Serpersu, Fred C. Hartman Probing the roles of conserved histidine residues in β-galactosidase ( E. coli ) using site directed mutagenesis and transition state analog inhibition Original Research Article Pages 365-371 Nathan J. Roth, Katherine Y.N. Wong, Reuben E. Huber Rapid in vitro assembly of class I major histocompatibility complex Original Research Article Pages 375-383 Nicholas J. Papadopoulos, James C. Sacchettini, Stanley G. Nathenson, Ruth Hogue Angeletti Peptide models of bZIP proteins: Quantitative analysis of DNA affinity and specificity Original Research Article Pages 385-391 Steven J. Metallo, Alanna Schepartz Applying affinity coelectrophoresis to the study of non-specific, DNA binding peptides Original Research Article Pages 393-400 Michael L. Nedved, Gregory R. Moe Investigating calmodulin-target sequence interactions using mutant proteins and synthetic target peptides Original Research Article Pages 401-408 Wendy A. Findlay, Stephen R. Martin, Peter M. Bayley Interactions of bacterial cell-surface proteins with antibodies: a ersatile set of protein-protein interactions Original Research Article Pages 409-416 Gordon C.K. Roberts, Lu-Yun Lian, Igor L. Barsukov, Jeremy P. Derrick, Koichi Kato, Yoji Arata Studies of cytokine-cytokine receptor interactions: Influence of ligand dimerization Original Research Article Pages 417-425 Larry D. Ward, Geoffrey J. Howlett, Robert L. Moritz, Annet Hammacher, Kiyoshi Yasukawa, Richard J. Simpson New high sensitivity sedimentation methods: Application to the analysis of the assembly of bacteriophage P22 Original Research Article Pages 427-432 Walter F. Stafford III, Sen Liu, Peter E. Prevelige Jr. Cyanogen as a conformational probe Original Research Article Pages 435-442 Richard A. Day, Amy Hignite, Warren E. Gooden Evaluation of interactions between residues in α-helices by exhaustive conformational search Original Research Article Pages 443-450 Trevor P. Creamer, Rajgopal Srinivasan, George D. Rose Design, synthesis and characterization of a water-soluble β-sheet peptide Original Research Article Pages 451-457 David S. Wishart, Les H. Kondejewski, Paul D. Semchuk, Cyril M. Kay, Robert S. Hodges, Brian D. Sykes Automated analysis of protein folding Original Research Article Pages 459-466 Richard A. Smith, Jack Henkin, Thomas F. Holzman Hsp70-protein complexes: Their characterization by size-exclusion HPLC Original Research Article Pages 467-474 Daniel R. Palleros, Li Shi, Katherine Reid, Anthony Fink Methods for collecting and analyzing attenuated total reflectance FTIR spectra of proteins in solution Original Research Article Pages 475-484 Keith A. Oberg, Anthony L. Fink 19 F NMR studies of fluorinated sugars binding to the glucose and galactose receptor Original Research Article Pages 487-494 Linda A. Luck Heteronuclear gradient-enhanced NMR for the study of 20–30kDa proteins: Application to human carbonic anhydrase II Original Research Article Pages 495-502 Ronald A. Venters, Leonard D. Spicer Toward the solution structure of large (>30 kDa) proteins and macromolecular complexes Original Research Article Pages 503-510 Cheryl H. Arrowsmith, Weontae Lee, Matthew Revington, Toshio Yamazaki, Lewis E. Kay Solution structures of horse ferro- and ferricytochrome c using 2D and 3D 1 H NMR and restrained simulated annealing Original Research Article Pages 511-519 Phoebe X. Qi, Ernesto J. Fuentes, Robert A. Beckman, Deena L. Di Stefano, A. Joshua Wand NMR relaxation methods to study ligand-receptor interactions Original Research Article Pages 521-528 David W. Hoyt, Jian-Jun Wang, Brian D. Sykes Application of 2-chlorotrityl resin: Simultaneous synthesis of peptides which differ in the C-termini Original Research Article Pages 531-538 Anita L. Hong, Tin T. Le, Trung Phan Correlation of cleavage techniques with side-reactions following solid-phase peptide synthesis Original Research Article Pages 539-546 Gregg B. Fields, Ruth H. Angeletti, Lynda F. Bonewald, William T. Moore, Alan J. Smith, John T. Stults, Lynn C. Williams Protein synthesis on a solid support using fragment condensation Original Research Article Pages 547-554 Siegfried Brandtner, Christian Griesinger Characterization of a side reaction using stepwise detection in peptide synthesis with Fmoc chemistry Original Research Article Pages 555-562 Yan Yang, William V. Sweeney, Susanna Thörnqvist, Klaus Schneider, Brian T. Chait, James P. Tam High sensitivity peptide sequence analysis using in Situ proteolysis on high retention PVDF membranes and a biphasic reaction column sequencer Original Research Article Pages 565-574 Sandra Best, David F. Reim, Jacek Mozdzanowski, David W. Speicher Index Pages 575-585 TECHNIQUES IN PROTEIN CHEMISTRY VI, an invaluable bench-top reference source for protein chemists, highlights current methods in the following areas: Protein sequencing and amino acid analysis Mass spectral analysis of peptides and proteins Posttranslational processing High-sensitivity protein and peptide separations Protein folding and NMR Analysis of protein interactions Protein design and engineering Techniques in Protein Chemistry VI, an invaluable bench-top reference source for protein chemists, highlights current methods in the following areas: * Protein sequencing and amino acid analysis * Mass spectral analysis of peptides and proteins * Posttranslational processing * High-sensitivity protein and peptide separations * Protein folding and NMR * Analysis of protein interactions * Protein design and engineering