Systems Biology of RNA Binding Proteins (Advances in Experimental Medicine and Biology Book 825)

"After transcription in the nucleus, RNA binding proteins (RBPs) recognize cis-regulatory RNA elements within pre-mRNA sequence to form mRNA-protein (mRNP) complexes Similarly to DNA binding proteins such as transcription factors that regulate gene expression by binding to DNA elements in the promoters of genes, RBPs regulate the fate of target RNAs by interacting with specific sequences or RNA secondary structural features within the transcribed RNA molecule The set of functional RNA elements recognized by RBPs within target RNAs and which control the temporal, functional and spatial dynamics of the target RNA define a putative “mRNP code” These cis-regulatory RNA elements can be found in the 5’ and 3’ untranslated regions (UTRs), introns, and exons of all protein-coding genes RNA elements in 5’ and 3’ UTRs are frequently involved in targeting RNA to specific cellular compartments, affecting 3’ end formation, controlling RNA stability and regulating mRNA translation RNA elements in introns and exons are known to function as splicing enhancers or silencers during the splicing process from pre-mRNA to mature mRNA This book provides case studies of RNA binding proteins that regulate aspects of RNA processing that are important for fundamental understanding of diseases and development Chapters include systems-level perspectives, mechanistic insights into RNA processing and RNA Binding proteins in genetic variation, development and disease The content focuses on systems biology and genomics of RNA Binding proteins and their relation to human diseases - Font no determinada Front Matter....Pages i-x Evolutionary Conservation and Expression of Human RNA-Binding Proteins and Their Role in Human Genetic Disease....Pages 1-55 The Functions and Regulatory Principles of mRNA Intracellular Trafficking....Pages 57-96 RNA-Binding Proteins in Regulation of Alternative Cleavage and Polyadenylation....Pages 97-127 Functional Analysis of Long Noncoding RNAs in Development and Disease....Pages 129-158 Piwi Proteins and piRNAs Step onto the Systems Biology Stage....Pages 159-197 Manipulation of RNA Using Engineered Proteins with Customized Specificity....Pages 199-225 Genetic Variation and RNA Binding Proteins: Tools and Techniques to Detect Functional Polymorphisms....Pages 227-266 Genome-Wide Activities of RNA Binding Proteins That Regulate Cellular Changes in the Epithelial to Mesenchymal Transition (EMT)....Pages 267-302 Antisense Oligonucleotide-Based Therapies for Diseases Caused by pre-mRNA Processing Defects....Pages 303-352 RNA-Binding Protein Misregulation in Microsatellite Expansion Disorders....Pages 353-388 RNA-Binding Proteins in Heart Development....Pages 389-429 Identification of miRNAs and Their Targets in C. elegans ....Pages 431-450 Splicing Code Modeling....Pages 451-466 Back Matter....Pages 467-468