Structure–Function Relationships of Proteolytic Enzymes. Proceedings of the International Symposium, Copenhagen, June 16–18, 1969, No. 37 in the Series of the International Union of Biochemistry Sponsored Symposia

Structure–Function Relationships of Proteolytic Enzymes provides information pertinent to the fundamental aspects of proteolytic enzymes. This book presents the historical role of proteolytic enzyme as a group in protein and enzyme chemistry. Organized into 23 chapters, this book begins with an overview of the results obtained from investigation on the chymotrypsinogens of porcine origin. This text then examines the differences of amino acid sequence between chymotrypsin, trypsin, and elastase that affect the substrate binding site, which reflect the specificity differences between these enzymes. Other chapters consider the kinetic parameters related to the trypsin-catalyzed hydrolysis of several model peptides. This book discusses as well the acetylation of trypsin, which result in functional consequences varying from complete inactivation to promotion of activity. The final chapter deals with the physical properties of stem bromelain in comparison with the data for three other sulfhydryl proteases of plant origin. This book is a valuable resource for enzymologists, microbiologists, and biochemists. Content: Front Matter, Page 3 Copyright, Page 4 Preface, Page 5, Pierre Desnuelle, Hans Neurath, Martin Ottesen List of Participants, Pages 11-15 Foreword, Pages 17-20, HANS NEURATH On the Three Chymotrypsinogens of Porcine Pancreas, Pages 21-35, P. DESNUELLE, D. GRATECOS, M. CHARLES, R. PEANASKY, J. BARATTI, M. ROVERY Chymotrypsin: Tertiary Structure and Enzymatic Activity, Pages 36-41, D.M. BLOW The Role of Carboxylates and Phenol Side-chains in the Activity and Conformation of Trypsinogen, Trypsin, Chymotrypsinogen, and Chymotrypsin, Pages 42-55, M. LAZDUNSKI, M. DELAAGE, J.P. ABITA, J.P. VINCENT Chemical Modifications of Bovine Trypsinogen and Trypsin, Pages 56-69, K.A. WALSH, L.L. HOUSTON, R.A. KENNER The Specificity Site of Trypsin, Pages 70-79, ELLIOTT SHAW Structure and Mechanism of Action of a Pancreatic Trypsin Inhibitor, Pages 80-88, R. ACHER, J. CHAUVET The Chemistry of the Reactive Site of Soybean Trypsin Inhibitor, Pages 89-101, MICHAEL LASKOWSKI JR. Structural Aspects of Interaction of Trypsin with Macromolecular Inhibitors, Pages 102-112, BOŘIVOJ KEIL Homology and Phylogeny of Proteolytic Enzymes, Pages 113-137, HANS NEURATH, RALPH A. BRADSHAW, RUTH ARNON Structural Aspects of Thrombin and Prothrombin, Pages 138-143, STAFFAN MAGNUSSON Cobalt Proteases: Implications of Absorption and Circular Dichroism Spectra for Catalysis, Pages 144-159, B.L. VALLEE, S.A. LATT Some Structure-Function Relationships in the Subtilisins, Pages 160-172, EMIL L. SMITH, FRANCIS S. MARKLAND, A.N. GLAZER Subtilisin BPN': Tertiary Structure and Inhibitor Binding, Pages 173-174, R.A. ALDEN, C.S. WRIGHT, F.C. WESTALL, J. KRAUT Subtilisin: Stability Properties and Secondary Binding Sites, Pages 175-187, M. OTTESEN, J.T. JOHANSEN, I. SVENDSEN Aminopeptidases from Thermophilic Microorganisms, Pages 188-197, HERBERT ZUBER A Synthetic Approach to the Study of Microenvironmental Effects on Enzyme Action: The Second Kaj Linderstrøm-Lang Gold Medal Lecture, Pages 198-221, E. KATCHALSKI Specificity and Mechanism of Pepsin Action, Pages 222-236, JOSEPH S. FRUTON Towards a Mechanism for Pepsin, Pages 237-252, J.R. KNOWLES, R.S. BAYLISS, A.J. CORNISH-BOWDEN, P. GREENWELL, T.M. KITSON, HILARY C. SHARP, GRITH B. WYBRANDT Chemical Studies on Purified Pepsin, Pages 253-260, WILLIAM H. STEIN Porcine Pepsinogen and Pepsin, Pages 261-271, GERTRUDE E. PERLMANN The Three-Dimensional Structure of Papain, Pages 272-288, B.G. WOLTHERS, J. DRENTH, J.N. JANSONIUS, R. KOEKOEK, H.M. SWEN Streptococcal Proteinase, Pages 289-297, STANFORD MOORE Structure and Function of Stem Bromelain, Pages 298-309, TAKASHI MURACHI, NORIKO TAKAHASHI