Structural And Contractile Proteins, Part C: The Contractile Apparatus And The Cytoskeleton, Volume 134: Volume 134: Structural And Contractile Proteins Part C (methods In Enzymology)

The critically acclaimed laboratory standard, **Methods in Enzymology**, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences Content: Contributors to volume 134 Pages xi-xv Preface Pages xvii-xviii Richard B. Vallee Volumes in series Pages xix-xxxi [1] Preparation and assay of cytoplasmic and secreted gelsolin Original Research Article Pages 3-9 H.L. Yin [2] Purification of severin Original Research Article Pages 9-13 Susan S. Brown [3] Isolation of fascin, and actin-bundling protein, and SU45, an actin-severing/capping protein from sea urchin eggs Original Research Article Pages 13-23 Joseph Bryan [4] Purification of the intestnal microvillus cytoskeletal proteins villin, fimbrin, and ezrin Original Research Article Pages 24-37 Anthony Bretscher [5] Purification of caldesmon Original Research Article Pages 37-42 William Lynch, Anthony Bretscher [6] Purification of fodrin from mammalian brain Original Research Article Pages 42-54 Richard Cheney, Joel Levine, Mark Willard [7] Purification of brain analogs of red blood cell membrane skeletal proteins: Ankyrin, protein 4.1 (synapsin), spectrin, and spectrin subunits Original Research Article Pages 55-69 Vann Bennett, Anthony J. Baines, Jonathan Davis [8] Purification and assay of vinculin, metavinculin, and talin Original Research Article Pages 69-77 T. O'Halloran, L. Molonoy, K. Burridge [9] Isolation of metavinculin from chicken smooth muscle Original Research Article Pages 78-85 Janet D'Angelo Siliciano, Susan W. Craig [10] Reversible assembly purification of microtubules without assemly-promoting agents and further purification of tubulijn, microtubule-associated proteins, and MAP fragments Original Research Article Pages 89-104 Richard B. Vallee [11] Purification of brain microtubules and microtubule-associated protein 1 using taxol Original Research Article Pages 104-115 Richard B. Vallee [12] Purification of microtubules and microtubule-associated proteins from sea urchin eggs and cultured mammalian cells using taxol, and use of exogenous taxol-stabilized brain microtubules for purifying microtubule-associated proteins Original Research Article Pages 116-127 Richard B. Vallee, Christine A. Collins [13] Isolation of sea urchin egg tubulin Original Research Article Pages 128-138 H. William Detrich III [14] Direct identification of microtubule-associated proteins be selective extraction of cultured cells Original Research Article Pages 139-147 Frank Solomon [15] Purification and assay of primate 210K microtubule-associated protein Original Research Article Pages 147-156 Jeannette Chloë Bulinski [16] Purification of tau protein from brain Original Research Article Pages 156-160 David Drubin, Marc Kirschner [17] Purification and assay of cold-stable microtubules and STOP protein Original Research Article Pages 160-170 Robert L. Margolis, Charles T. Rauch, Didier Job [18] Isolation and purification of tubulin tyrosine ligase Original Research Article Pages 170-179 Jürgen Wehland, Heinz C. Schröder, Klaus Weber [19] Proteolytic modification of tubulin Original Research Article Pages 179-190 Luis Serrano, Francisco Wandosell, Javier de la Torre, Jesús Avila [20] Isolation of sea urchin spindles and cytasters Original Research Article Pages 190-199 Ryoko Kuriyama [21] Isolation of native, membrane-containing mitotic apparatus from sea urchin embryos Original Research Article Pages 200-217 Robert B. Silver [22] Isolation of mitotic spindles from mammalian cells Original Research Article Pages 217-225 R.C. Brady, M.J. Schibler, F. Cabral [23] Isolation of mitotic spindles and spindle pole bodies from yeast Original Research Article Pages 225-232 Jeremy S. Hyams, Stephen M. King [24] Isolation of the erythrocyte marginal band Original Research Article Pages 232-252 William D. Cohen, Mary F. Ginsburg [25] Isolation of basal bodies from Chlamydomonas Original Research Article Pages 252-261 William J. Snell [26] Isolation of mammalian centrosomes Original Research Article Pages 261-268 T.J. Mitchison, M.W. Kirschner [27] Isolation of a kinetochore—centromere fraction from HeLa metaphase chromosomes Original Research Article Pages 268-280 Manuel M. Valdivia, B.R. Brinkley [28] Isolation of Chlamydomonas flagella and flagellar axonemes Original Research Article Pages 280-290 George B. Witman [29] Purification and characterization of Chlamydomonas flagellar dyneins Original Research Article Pages 291-306 Stephen M. King, Tim Otter, George B. Witman [30] Preparation and properties of dynein from Tetrahymena cilia Original Research Article Pages 306-317 Kenneth A. Johnson [31] Extraction and properties of dyneins from bull spermatozoa Original Research Article Pages 318-324 Claude Gagnon [32] Purification of cytoplasmic dynein from Strongylocentrotus sea urchin eggs Original Research Article Pages 325-337 M.M. Pratt [33] Purification of cytoplasmic dynein from sea urchin eggs Original Research Article Pages 337-351 Shin-ichi Hisanaga, Hikoichi Sakai [34] Separation of cytokeratin polypeptides by gel electrophoretic and chromatographic techniques and their identification by immunoblotting Original Research Article Pages 355-371 Thomas Achtstaetter, Mechthild Hatzfeld, Roy A. Quinlan, David C. Parmelee, Werner W. Franke [35] Isolation of intermediate filaments from neuronal tissues by cycles of assembly/disassembly Original Research Article Pages 371-379 Robert V. Zackroff [36] Purification of neurofilaments and their constituent polypeptides Original Research Article Pages 380-388 Ronald K.H. Liem [37] Isolation of paired helical filaments and amyloid fibers from human brain Original Research Article Pages 388-404 Dennis J. Selkoe, Carmela R. Abraham [38] Methods for studying spasmin Original Research Article Pages 404-408 W. Brad Amos [39] Isolation of the calcium-modulated contractile protein of striated flagellar roots Original Research Article Pages 408-414 J.L. Salisbury, K.W. Aebig, D.E. Coling [40] Isolation of nematode major sperm proteins Original Research Article Pages 414-420 Samuel Ward, Michael R. Klass [41] Antibody inhibitors of nonmuscle myosin function and assembly Original Research Article Pages 423-453 Daniel P. Kiehart, Donald A. Kaiser, Thomas D. Pollard [42] Preparation of antibodies reactive with specific regions of cytoskeletal proteins Original Research Article Pages 453-460 Jeannette Chloë Bulinski, Gregg G. Gundersen [43] Purification of isoform-selective actin antibody from polyclonal antiserum Original Research Article Pages 460-467 Susan W. Craig, Sheila K. Sanders, JoséV. Pardo [44] Analysis of cytoskeletal structures using blot-purified monospecific antibodies Original Research Article Pages 467-472 J.B. Olmsted [45] Preparation of N -ethylmaleimide-modified heavy meromyosin and its use as a functional probe of actomyosin-based motility Original Research Article Pages 473-477 W. Zacheus Cande [46] Erythro-9-[3-(2-hydroxynonyl)]adenine and Vanadate as probes for microtubule-based cytoskeletal mechanochemistry Original Research Article Pages 477-487 Stephen M. Penningroth [47] Preparation of a fluorescent analog: Acetamidofluoresceinyl-labeled Dictyostelium discoideum α-actinin Original Research Article Pages 487-507 John R. Simon, D. Lansing Taylor [48] Preparation, assay, and microinjection of fluorescently labeled cytoskeletal proteins: Actin, α-actinin, and vinculin Original Research Article Pages 507-519 Thomas E. Kreis [49] Preparation and characterization of fluorescent analogs of tubulin Original Research Article Pages 519-528 Patricia Wadsworth, E.D. Salmon [50] Myosin movement in Vitro: A quantitative assay using oriented actin cables from Nitella Original Research Article Pages 531-544 Michael P. Sheetz, Steven M. Block, James A. Spudich [51] Methods to study the microtubule-neurofilament network in Vitro Original Research Article Pages 544-555 Eric J. Aamodt, Robley C. Williams Jr. [52] Gel overlay methods for detecting specific protein-protein interactions Original Research Article Pages 555-560 Joann J. Otto [53] Viewing single microtubules by video light microscopy Original Research Article Pages 561-573 Bruce J. Schnapp [54] Agar overlay method: High-resolution immunofluorescence for the study of the contractile apparatus Original Research Article Pages 573-580 Yoshio Fukui, Shigehiko Yumura, Toshiko K. Yumura, Hiroshi Mori [55] Polyethylene glycol embedding protocol for immunofluorescence microscopy Original Research Article Pages 580-591 John J. Wolosewick [56] Preembedding for localization by electron microscopy of cytoskeletal antigens in cultured cell monolayers using gold-labeled antibodies Original Research Article Pages 592-597 J. De Mey, G. Langanger, G. Geuens, R. Nuydens, M. De Brabander [57] Quick freeze, deep etch of the cytoskeleton Original Research Article Pages 598-612 Nobutaka Hirokawa [58] Cryo-electron microscopy of unstained frozen-hydrated microtubules Original Research Article Pages 612-633 Eva-Maria Mandelkow, Eckhard Mandelkow [59] X-ray diffraction of cytoskeletal fibers Original Research Article Pages 633-657 Eckhard Mandelkow [60] Time-resolved x-ray scattering of microtubule assembly using synchrotron radiation Original Research Article Pages 657-676 Eckhard Mandelkow, Joan Bordas [61] Rapid kinetic analysis of mechanochemical adenosinetriphosphatases Original Research Article Pages 677-705 Kenneth A. Johnson Author index Pages 707-727 Subject index Pages 729-748