Steroid Analysis

The second edition of this book has drawn heavily on the first edition but a huge amount of research on steroid analysis has been published over the last 15 years. As a result, the Editors decided to let the first edition of this book stand on its own and direct readers interested in pre-1995 steroid analysis to it, simply because the post-1995 research can on its own fill the second edition. We have tried to keep a balance but equally have allowed authors of each chapter a significant degree of freedom to approach their particular topics as they thought fit – they are after all the experts in their field. We hope that readers will agree that we have got the balance right. In re-writing or updating these chapters, we have been greatly assisted by the developments in the availability of research publications electronically. Huge strides have been made in this area since 1995 and the ability to read a paper on one’s computer rather than trekking to the British Library is a tremendous advantage. The editors wish to express their gratitude to their respective insti- tions (St. Bartholomew’s and the Royal London School of Medicine, Queen Mary University of London and Kings College London) for providing us with el- tronic access to research journals from our home computers. Without such access, it would have been impossible to complete this book. This comprehensive and up-to-date 2nd edition of the Steroid Analysis handbook, written by a team of distinguished experts from around the world concentrates on the analysis of steroids in biological fluids. This edition is based on the same format as the first, primarily dealing with analysis of low levels of steroid analytes in biological fluids, but including an extra chapter on pharmaceutical aspects of steroid analysis. Spectroscopic and other methods, including UV and IR absorption spectroscopy, NMR spectroscopy, mass spectrometry, X-ray diffraction, chromatography and immunoassay of steroids are treated in detail. Discussion of general methods for extraction, purification and quantitation is followed by consideration of the analysis of specific important steroid groups, such as progestagens, estrogens, corticosteroids, vitamins D, bile acids and anabolic steroids and their metabolites. The editors have made a conscious decision to leave the first edition to stand on its own with regards to steroid analysis prior to 1995 and authors therefore were asked to review the modern post-1995 steroid scene, though pre-1995 data were not excluded if authors felt it was necessary. This book should be of interest to analytical chemists, biochemists and chemical pathologists with an interest or involvement in steroids. Content: Front Matter....Pages I-XXII Structure and Nomenclature of Steroids....Pages 1-25 Spectroscopic Methods of Steroid Analysis....Pages 27-161 General Methods for the Extraction, Purification, and Measurement of Steroids by Chromatography and Mass Spectrometry....Pages 163-282 Immunoassay of Steroids....Pages 283-327 Analysis of Corticosteroids....Pages 329-455 Analysis of Androgens and Their Derivatives....Pages 457-558 Analysis of Progestagens....Pages 559-603 The Measurement of Estrogens....Pages 605-742 Anabolic Steroids: Metabolism, Doping and Detection in Human and Equestrian Sports....Pages 743-836 Analysis of Bile Acids....Pages 837-966 Analysis of Vitamins D, Their Metabolites and Analogues....Pages 967-1096 Industrial and Compendial Steroid Analysis....Pages 1097-1153 Quality Management of Steroid Hormone Assays....Pages 1155-1188 Back Matter....Pages 1189-1224 This comprehensive and up-to-date 2nd edition of the Steriod Analysis handbook, written by a team of distinguished experts from around the world concentrates on the analysis of steroids in biological fluids. This edition is based on the same format as the first, primarily dealing with analysis of low levels of steroid analytes in biological fluids, but including an extra chapter on pharmaceutical aspects of steroid analysis. Spectroscopic and other methods, including UV and IR absorption spectroscopy, NMR spectroscopy, mass spectrometry, X-ray diffraction, chromatography and immunoassay of ste The second edition of this handbook concentrates on the analysis of steroids in biological fluids. It offers analysis of low levels of steroid analytes in biological fluids. This new edition also provides an extra chapter on pharmaceutical aspects of steroid analysis. Coverage details spectroscopic and other methods, including UV and IR absorption spectroscopy, NMR spectroscopy, mass spectrometry, X-ray diffraction, chromatography and immunoassay of steroids.