Stability and stabilization of biocatalysts : proceedings of an international symposium organized under auspices of the Working Party on Applied Biocatalysis of the European Federation of Biotechnology, the University of Cordoba, Spain, and the Spanish So

Six years after the symposium on Stability and Stabilization of Enzymes , a second symposium, Stability and Stabilization of Biocatalysts , on which this book is based, was organized. At the symposium, 210 participants representing all continents came together to learn from 150 oral and poster communications. The volume brings up-to-date the work already going on, and identifies possible breakthroughs in the research. This timely book therefore presents cutting edge developments in topics such as non-covalent processes in solution, protein engineering and thermophile enzymes, immobilized enzymes, non-conventional media, and whole cells. An excellent addition to the available literature, it will make a useful contribution to this key area of applied biocatalysis. Content: Preface Page v Acknowledgements Page vii Diagnosing the inactivating process of enzymes Original Research Article Pages 3-18 Onno Misset, Alard van Dijk Stability and stabilization of glucose-fructose oxidoreductase from Zymomonas mobilis against irreversible inactivation during substrate turnover in biochemical reactors Original Research Article Pages 19-26 B. Nidetzky, M. Fürlinger, D. Haltrich, K.D. Kulbe Reactor performance under thermal inactivation and temperature optimization with chitin-immobilized lactase Original Research Article Pages 27-34 A. Illanes, L. Wilson, C. Altamirano, A. Aillapán Fast in-situ-characterisation of biocatalysts long-term stability Original Research Article Pages 35-40 M. Boy, A. Dominik, H. Voss Chemical and thermal stability of ferulic acid (feruloyl) esterases from Aspergillus Original Research Article Pages 41-46 C.B. Faulds, F.O. Aliwan, R.P. de Vries, R.W. Pickersgill, J. Visser, G. Williamson Stability properties of calf intestinal alkaline phosphatase Original Research Article Pages 47-52 Ultan McKeon, Brendan O'Connor, Ciarán Ó'Fágáin Stabilization of β-glucanase and its effect on the substrate hydrolysis pattern Original Research Article Pages 53-58 I. Marković, B. Marković-Devčić, S. Gamulin, N. Pavlović Some factors affecting the behavior of anhydrous α-chymotrypsin at high temperature Original Research Article Pages 59-64 Domenico Pirozzi Guido Greco jr. Horseradish peroxidase stability in the course of phenols oxidation Original Research Article Pages 65-70 A.M. Egorov, Yu.L. Kapeluich, T.A. Pastuhova, M.Yu. Rubtsova, D.N. Cherepanov The effect of impurities of crude olive residue oil on the operational stability of the Candida rugosa lipase immobilized in polyurethane foams Original Research Article Pages 71-76 A.C. Correia, S. Ferreira-Dias Approaches for improved identification of mechanisms of enzyme inactivation Original Research Article Pages 77-82 M. Polakovič, P. Vrábel, V. Báleš Stability and stabilization of α-1,4-D-glucan phosphorylases Original Research Article Pages 83-88 R. Grieβler, B. Müller-Fembeck, S. D'Auria, F. La Cara, B. Nidetzky Oxidation by hydrogen peroxide of D-amino acid oxidase from Rhodotorula gracilis Original Research Article Pages 89-94 V. Obregón, I. de la Mata, F. Ramón, C. Acebal, M.P. Castillón Cosolvent effect on the synthesis of ampicillin and cephalexin with penicillin acylase Original Research Article Pages 95-100 C. Aguirre, J. Baeza, A. Illanes Conversion and stability studies on enzyme-membrane reactor with lipase immobilized by different methods Original Research Article Pages 101-106 T. Venyige, E. Csányi, Cs. Sisak Study of the deactivation process of the glucose oxidase-catalase enzymatic system by means of simulation Original Research Article Pages 107-112 L.E. Romero, D. Cantero Stabilization of hydrolases by chemical modification with fatty acids or polyethylene glycol Original Research Article Pages 115-120 F.J. Plou, M.V. Calvo, M. Ferrer, A. Ballesteros Modification of the carbohydrate moiety in ribonuclease B and its influence on the protein stability probed by limited proteolysis Original Research Article Pages 121-126 Ulrich Arnold, Renate Ulbrich-Hofmann Comparative studies on selective modification of e-amino groups in Upases and phospholipase A 2 Original Research Article Pages 127-134 Bart C. Koops, Arend J. Slotboom, Hubertus M. Verheij Analysis of the thermal deactivation kinetics of α-chymotrypsin modified by chemoenzymatic glycosylation Original Research Article Pages 135-140 María Asunción Longo, Didier Combes Increasing the operational stability of flavoproteins by covalent cof actor binding Original Research Article Pages 141-146 Willem J.H. van Berkel, Robert H.H. van den Heuvel, Marco W. Fraaije, Colja Laane Properties of PEG-modified microbial proteases. Activity and stability studies Original Research Article Pages 147-150 T.M. Fatum, A.Agerlin Olsen, C.C. Fuglsang, D. Otzen The effect of crosslinking on thermal inactivation of cellulases Original Research Article Pages 151-156 J. Bilen, U. Bakir Studies on the stability of acid phosphatase ( a. niger ) by crosslinking with glutaraldehyde and soil humates Original Research Article Pages 157-161 N. Ortega, L. Berzal, M.D. Busto, M. Perez-Mateos Folding and association versus misfolding and aggregation of proteins Original Research Article Pages 165-182 R. Jaenicke Activity of monoclonal antibodies in prevention of in vitro aggregation of their antigens Original Research Article Pages 183-188 Beka Solomon, Tamar Katzav-Gozanski, Rela Koppel, Eilat Hanan-Aharon Stability of monodeamidated forms of ribonuclease A Original Research Article Pages 189-196 F. Catanzano, G. Graziano, S. Capasso, G. Barone Pressure effects on protein oligomeric dissociation Original Research Article Pages 197-204 C. Balny Effect of high hydrostatic pressure on enzyme stability Original Research Article Pages 205-210 V. Athès, D. Combes Thermodynamic stability of a monomeric derivative of bovine seminal ribonuclease Original Research Article Pages 211-216 G. Barone, F. Catanzano, G. Graziano, V. Cafaro, G. D'Alessio, A. Di Donato Thermodynamic stability of ribonuclease P2 from Sulfolobus solfataricus and some mutants Original Research Article Pages 217-222 G. Graziano, F. Catanzano, P. Fusi, P. Tortora, G. Barone Stabilization of α-Chymotrypsin by DMSO Original Research Article Pages 223-228 E. Flaschel, L. Ebmeier Thermal stabilization by its ligands of NADP + -isocitrate dehydrogenase from the thermophilic cyanobacterium Phormidium laminosum Original Research Article Pages 229-234 Miguel A. Pardo, María J. Llama, Juan L. Serra The characterization of protein stability from hydrogen exchange : Maximum entropy analysis of co-solvent effects on exchange rates Original Research Article Pages 235-240 Badredine Souhail, Isabel M. Plaza del Pino, Jose M. Sanchez-Ruiz Preferential hydration changes upon protein unfolding in water-cosolvent mixtures Original Research Article Pages 241-245 Hassan O. Hammou, Isabel M. Plaza del Pino, Jose M. Sanchez-Ruiz Characterizing protein-cosolvent interactions coupled to protein refolding by kinetic calorimetry Original Research Article Pages 247-250 Mohammed C. Boulaich, Antonio Parody-Morreale, M.Sánchez-Ruiz José Characterizing cosolvent effects on protein stability: cold-denaturation of ubiquitin in the presence of guanidine Original Research Article Pages 251-255 Beatriz Ibarra Molero, Jose M. Sanchez-Ruiz Acetylcholinesterase-ethanol interactions: inactivation, substrate and ligand exclusion Original Research Article Pages 257-262 F. Ortéga, D. Garcia, J.-L. Marty Effect of a mixed stabilizer-denaturant system on the stability of enzyme activity at high temperatures : lysozyme as a model Original Research Article Pages 263-268 Y. Sangeeta Devi, Usha B. Nair, Rajiv Bhat A simple folding method for high level production of the hydrophobic disulfide bonded hepatitis B X protein by inclusion body route and its structural analysis Original Research Article Pages 269-274 I. Marczinovits, J. Molnár, M.Z. Kele, P.T. Szabó, T. Janáky Rigidity of thermophilic enzymes Original Research Article Pages 277-294 Angelo Fontana, Vincenzo De Filippis, Patrizia Polverino de Laureto, Elena Scaramella, Marcello Zambonin Improvement of thermal stability of a diagnostic enzyme, Streptomyces cholesterol oxidase, by random and site-directed mutageneses and a structural interpretation Original Research Article Pages 295-302 Y. Murooka, Y. Nishiya, M. Toyama, M. Aoike, M. Yamashita, N. Hirayama Protein engineering for thermostabilisation of proteins: some theoretical rules and application to a β-glucanase Original Research Article Pages 303-310 E. Querol, J. Pons, J. Cedano, M. Vallmitjana, F. García, C. Bonet, J. Pérez-Pons, A. Planas, A. Mozo-Villarías Mechanisms of stabilization of the β-glycosidase from the hyperthermophilic archaeon Sulfolobus solfataricus Original Research Article Pages 311-316 M. Moracci, M. Ciaramella, L.-H. Pearl, B. Cobucci Ponzano, M. Rossi A strategy for engineering thermostability: the case of cyclodextrin glycosyltransferase Original Research Article Pages 317-323 Joost C.M. Uitdehaag, Bauke W. Dijkstra Thermophilic esterases and the amino acid “traffic rule” in the hormone sensitive lipase subfamily Original Research Article Pages 325-330 Giuseppe Manco, Ferdinando Febbraio, Mosè Rossi Cloning and stabilization of NAD-dependent formate dehydrogenase from candida boidinii by site-directed mutagenesis Original Research Article Pages 331-336 H. Slusarczyk, M. Pohl, M.-R. Kula Usefulness of bacterial thermostable enzymes in clinical chemical analysis Original Research Article Pages 337-342 K. Tomita, K. Nomura, T. Miura, H. Shibata Effect of temperature and Ca 2+ on the degree of multiple attack exhibited by mesophilic and thermophilic α-amylases Original Research Article Pages 343-347 B. Kramhøft, B. Svensson Degradation and denaturation of stable enzymes Original Research Article Pages 349-352 Dion Thompson, Roberto Fernández-Lafuente, Cesar Mateo, Don A. Cowan, Jose M. Guisan, Roy Daniel Engineering stability of enzymes in systems with organic solvents Original Research Article Pages 355-363 Vadim V. Mozhaev Inactivation of enzymes at the aqueous-organic interface Original Research Article Pages 365-372 Peter J Halling, Alistair C Ross, George Bell Exploiting hysteresis for high activity enzymes in organic media Original Research Article Pages 373-380 Johann Partridge, Peter.J. Halling, Barry D. Moore Limited proteolysis of proteins by thermolysin in trifluoroethanol Original Research Article Pages 381-392 Patrizia Polverino de Laureto, Elena Scaramella, Marcello Zambonin, Vincenzo De Filippis, Angelo Fontana Enzyme inactivation by inert gas bubbling Original Research Article Pages 393-398 Mylène Caussette, Alain Gaunand, Henri Planche, Brigitte Lindet Effects of water-miscible solvents on the stability and specificity of cyclodextringlucosyltransferases Original Research Article Pages 399-404 Anne D. Blackwood, Christopher Bucke Stabilization of immobilized enzymes against organic solvents: Complete hydrophylization of enzymes environments by solidphase chemistry with poly-functional macromolecules. Original Research Article Pages 405-410 R. Fernandez-Lafuente, C.M. Rosell, L. Caanan-Haden, L. Rodes, J.M. Guisan Effect of sorbitol on immobilized a-chymotrypsin thermostability in low-water system Original Research Article Pages 411-416 T.de Diego, P. Lozano, M.J. Ñíguez, J.L. Iborra. Stability of immobilized enzyme-polyelectrolyte complex against irreversible inactivation by organic solvents Original Research Article Pages 417-422 V. Levitsky, P. Lozano, A. Gladilin, J.L. Iborra Enhancement of invertase activity in organic media for oligosaccharide synthesis Original Research Article Pages 423-428 S. Bielecki, R.I. Somiari Effects of crown ethers on the activity of enzymes in peptide formation in organic media Original Research Article Pages 429-434 Dirk-Jan van Unen, Johan F.J. Engbersen, David N. Reinhoudt Recovery of the activity of an immobilized lipase after its use in fat transesterification Original Research Article Pages 435-440 S. Ferreira-Dias, C.S. Duarte, V. Falaschi, S.R. Marques, J.H. Gusmão, M.M.R. da Fonseca Effects of lipid-borne compounds on the activity and stability of lipases in microaqueous systems for the lipase-catalyzed interesterification Original Research Article Pages 441-446 X. Xu, C.-E. Høy, J. Adler-Nissen Stabilisation of lipases for activity in ammoniolysis Original Research Article Pages 447-452 F. van Rantwijk, A.C. Kock-van Dalen, R.A. Sheldon Water sorption isotherm as a tool to explore hydration of the microenvironment of biocatalysts Original Research Article Pages 453-458 J.M. Sánchez-Montero, R.M a de la Casa, J.V. Sinisterra Thermal stability of free and immobilised Pseudomonas cepacia lipase in aqueous and organic media Original Research Article Pages 459-464 G. Pencreac'h, Jacques C. Baratti The stabilization of enzyme in organic solvent at low temperature Original Research Article Pages 465-470 Hyon Joo Lee, Jin Ryun Kim, Young Je Yoo A comparative study of thermal inactivation of enzymes in supercritical carbon dioxide Original Research Article Pages 471-476 A. Gieβauf, T. Gamse, E. Klingsbichel, H. Schwab, R. Marr Studies on the stability of aminoacylase in some organic solvents Original Research Article Pages 477-482 L. Boross, J. Kosáry, E. Stefanovits-Bányai, C. Sisak, B. Szajáni Cutinase activity and enantioselectivity in supercritical fluids Original Research Article Pages 483-486 Nuno Fontes, M. Conceição Almeida, Sílvia Garcia, Célia Peres, João Grave, M. Raquel Aires-Barros, Cláudio M. Soares, Joaquim M.S. Cabral, Christopher D. Maycock, Susana Barreiros Effect of pressure on enzyme activity in compressed gases Original Research Article Pages 487-491 M.Conceição Almeida, Nuno Fontes, Eugénia Nogueiro, Sílvia Garcia, Célia Peres, António Silva, Manuel Carvalho, Susana Barreiros Activity and structural stability of adsorbed enzymes Original Research Article Pages 495-504 W. Norde, T. Zoungrana Covalent immobilization of glucose oxidase on AlPO 4 as inorganic support Original Research Article Pages 505-512 F.M. Bautista, M.C. Bravo, J.M. Campelo, A. García, D. Luna, J.M. Marinas, A.A. Romero. The effect of site-specific immobilization on the thermal stability of thermolysin-like neutral proteases Original Research Article Pages 513-516 J. Mansfeld, G. Vriend, B. Van den Burg, G. Venema, V.G.H. Eijsink, R. Ulbrich-Hofmann Immobilization of hydantoin cleaving enzymes from Arthrobacter aurescens DSM 3747 — Effect of the coupling method on the stability of the L-N-carbamoylase Original Research Article Pages 517-522 M. Pietzsch, H. Oberreuter, B. Petrovska, K. Ragnitz, C. Syldatk Thermoinactivation of β-xylosidase immobilized on nylon Original Research Article Pages 523-528 M.J. Dueñas, P. Estrada Increase of thermal stability of tannin acyl hydrolase by covalent immobilization through its carbohydrate side chain : application in tea cream solubilisation Original Research Article Pages 529-534 P. Nicolas, J.L. Sauvageat, S. Reymond, E. Raetz Stability in the presence of organic solvents of dextransucrase from Leuconostoc mesenteroides NRRL B-512F immobilized in calcium-alginate beads Original Research Article Pages 535-540 M. Alcalde, F.J. Plou, M.T. Martín, M. Remaud, P. Monsan, A. Ballesteros Immobilisation and characteristics of glucose oxidase immobilised on convective interaction media (CIM) disks Original Research Article Pages 541-546 A. Podgornik, M. Vodopivec, H. Podgornik, M. Barut, A. àtrancar Immobilization of spinach leaf hydroperoxide lyase Original Research Article Pages 547-552 L.M. Simon, Sz.J. Márczy, M. Kotormán, Sz.Á. Németh, B. Szajáni A New immobilization system for candida rugosa lipase: characterization and applications Original Research Article Pages 553-558 K. Carbone, M. Casarci Soybean lipoxygenases: Purification and stability of the free and immobilized enzymes Original Research Article Pages 559-564 Anthony Chikere, Boris Galunsky, Volker Kasche Stabilization of lipase B from Candida antarctica by immobilization on different supports Original Research Article Pages 565-570 M. Arroyo, J.M. Sánchez-Montero, J.V. Sinisterra Covalent immobilization of crude and partially-purified Upases onto inorganic supports: stability and hyperactivation Original Research Article Pages 571-576 A.R. Alcántara, I. Borreguero, M.T. López-Belmonte, J.V. Sinisterra. Immobilization of alkaline phosphatases on various supports Original Research Article Pages 577-582 B. Surinenaite, V. Bendikiene, B. Juodka Stabilization of serine proteases by immobilization Original Research Article Pages 583-588 V. Bendikiene, B. Juodka Immobilized cells: Plasmid stability and plasmid transfer Original Research Article Pages 591-602 J.N. Barbotin, D. Mater, M. Craynest, J.E. Nava Saucedo, N. Truffaut, D. Thomas Stabilization of immobilized cell systems using a modified metal surface, fructose polymer levan and a high cell concentration Original Research Article Pages 603-610 M. Bekers, E. Ventina, A. Karsakevich, I. Vina Plasmid stability in recombinant Saccharomyces cerevisiae expressing the EXG1 gene in free and immobilized cultures Original Research Article Pages 611-618 A. Guillán, T. Lú Chau, E. Roca, M.J. Núñez, J.M. Lema Bioreductions by Pyrococcus furiosus at elevated temperatures Original Research Article Pages 619-624 E. van den Ban, H. Willemen, H. Wassink, H. Haaker, C. Laane Stability of free and immobilized Mycobacterium sp. cells in aqueous and organic media Original Research Article Pages 625-630 P. Fernandes, J.M.S. Cabral, H.M. Pinheiro Operational stability of immobilized C. reinhardtii cells: an approach to its potential as biocatalyst for N-consuming processes Original Research Article Pages 631-638 I. Garbayo, C. Vílchez Induction and stability of cholesterol oxidase from cells of a Rhodococcus Original Research Article Pages 639-644 Joseph Kreit, Pierre Germain Stability of functionally active fusion proteins during their biosynthesis and isolation from expressing bacterial cells Original Research Article Pages 645-650 L.M. Vinokurov, A.V. Yakhnin, T.V. Ivashkina, V.N. Ksenzenko, Yu.B. Alakhov Production of bio-esters by immobilized lipases Original Research Article Pages 653-656 K. Bélafi-Bakó, L. Gubicza, E. Csányi, M.A Galán, J.A. Moreno An Aspergillus flavus strain promoting oleic acid esterification in isooctane Original Research Article Pages 657-660 V. Loscos, G. Cañes, B. Perpiña, M. Torres, N. Sala, R. Canela Covalent enzyme immobilization in different porous polymer membranes Original Research Article Pages 661-666 H.-G. Hicke, M. Becker, G. Maisch, M. Ulbricht Functionalised cross-linked polyvinyl alcohol as new matrix for lipase immobilization Original Research Article Pages 667-671 E. Cernia, G. Milana, G. Ortaggi, C. Palocci, S. Soro Six years after the symposium on Stability and Stabilization of Enzymes, a second symposium, Stability and Stabilization of Biocatalysts, on which this book is based, was organized. At the symposium, 210 participants representing all continents came together to learn from 150 oral and poster communications. The volume brings up-to-date the work already going on, and identifies possible breakthroughs in the research. This timely book therefore presents cutting edge developments in topics such as non-covalent processes in solution, protein engineering and thermophile enzymes, immobilized enzymes, non-conventional media, and whole cells. An excellent addition to the available literature, it will make a useful contribution to this key area of applied biocatalysis