Single Molecule Spectroscopy in Chemistry, Physics and Biology: Nobel Symposium (Springer Series in Chemical Physics, 96)

Written by the leading experts in the field, this book describes the development and current state-of-the-art in single molecule spectroscopy. The application of this technique, which started 1989, in physics, chemistry and biosciences is displayed. Front Matter....Pages I-XXI Front Matter....Pages 1-1 How Biomolecular Motors Work: Synergy Between Single Molecule Experiments and Single Molecule Simulations....Pages 3-22 Front Matter....Pages 24-24 Single-Molecule Optical Spectroscopy and Imaging: From Early Steps to Recent Advances....Pages 25-60 Single Molecules as Optical Probes for Structure and Dynamics....Pages 61-76 FCS and Single Molecule Spectroscopy....Pages 77-103 Front Matter....Pages 106-106 Single Molecule Spectroscopy Illuminating the Molecular Dynamics of Life....Pages 107-117 Chemical Fluxes in Cellular Steady States Measured by Fluorescence Correlation Spectroscopy....Pages 119-137 In Vivo Fluorescence Correlation and Cross-Correlation Spectroscopy....Pages 139-154 Fluorescence Flicker as a Read-out in FCS: Principles, Applications and Further Developments....Pages 155-172 Front Matter....Pages 174-174 Development of Nanocrystal Molecules for Plasmon Rulers and Single Molecule Biological Imaging....Pages 175-186 Size-Minimized Quantum Dots for Molecular and Cellular Imaging....Pages 187-201 Mapping Transcription Factors on Extended DNA: A Single Molecule Approach....Pages 203-216 Front Matter....Pages 218-218 Single Molecule Measurement, a Tool for Exploring the Dynamic Mechanism of Biomolecules....Pages 219-236 Viral DNA Packaging: One Step at a Time....Pages 237-269 Chemo-Mechanical Coupling in the Rotary Molecular Motor F 1 -ATPase....Pages 271-285 Front Matter....Pages 288-288 Mechanoenzymatics and Nanoassembly of Single Molecules....Pages 289-303 Single Cell Physiology....Pages 305-316 Force-Clamp Spectroscopy of Single Proteins....Pages 317-335 Unraveling the Secrets of Bacterial Adhesion Organelles Using Single-Molecule Force Spectroscopy....Pages 337-362 Front Matter....Pages 364-364 Far-Field Optical Nanoscopy....Pages 365-398 Sub-Diffraction-Limit Imaging with Stochastic Optical Reconstruction Microscopy....Pages 399-415 Front Matter....Pages 364-364 Assessing Biological Samples with Scanning Probes....Pages 417-431 Front Matter....Pages 434-434 Enzymology and Life at the Single Molecule Level....Pages 435-448 Controlling Chemistry in Dynamic Nanoscale Systems....Pages 449-468 Front Matter....Pages 470-470 Single-Molecule Protein Conformational Dynamics in Enzymatic Reactions....Pages 471-494 Watching Individual Enzymes at Work....Pages 495-511 The Influence of Symmetry on the Electronic Structure of the Photosynthetic Pigment-Protein Complexes from Purple Bacteria....Pages 513-533 Front Matter....Pages 536-536 Exploring Nanostructured Systems with Single-Molecule Probes: From Nanoporous Materials to Living Cells....Pages 537-551 Gene Regulation: Single-Molecule Chemical Physics in a Natural Context....Pages 553-560 Back Matter....Pages 561-572 Introductory lecture: Molecular dynamics of single molecules Detection of single molecules and single molecule processes Fluorescence-correlation spectroscopy Quantum dots and single molecule behavior Molecular motion of contractile elements and polymer formation Force and multiparameter spectroscopy on functional active proteins Nanosacale microscopy and high resolution imaging Single molecule miscroscopy in individual cells Catalysis of single enxyme molecules Fields and outlook. With A Foreword Written By A Nobel Laureate, This Book Describes The Development And Current State-of-the-art In Single Molecule Spectroscopy. The Application Of This Technique, Which Started 1989, In Physics, Chemistry And Biosciences Is Displayed. Written by the leading experts in the field, this book describes the development and current state of the art in single molecule spectroscopy. The application of this technique, which started 1989, in physics, chemistry and biosciences is displayed