Senescence: Dominant or Recessive in Somatic Cell Crosses? (Cellular Senescence and Somatic Cell Genetics)

This monograph, Senescence; Dominant or Recessive In Somatic Cell Crosses? represents the second annual workshop to promote theory and concept development in aging research. These workshops are part of a resource to bank cultured cells of special interest to aging research that was established at the Institute for Medical Research in Camden. New Jersey. by the National Institute on Aging in 1974. The underlying theme of the workshops is the use of cultured cells in a variety of somatic cell genetic systems designed to define mechanisms of in vitra cellular scen­ escence and the possible insights that this may provide to the problems of in viva aging. The concept also includes bringing together workers from a variety of disciplines to stimulate new and innovative thoughts and work in the area. The current work­ shop focuses on the relative role of nucleus and cytoplasm on determining the in vitra lifespan of human diploid cells as well as the relative influence of old and young cells when combined within a single cell structure. The techniques and procedures discussed should make significant contributions to understanding in vitra senescence and may lead to the mapping of an area or areas of the genome linked to senescence as is being accomplished with viral transformation of normal cells. Warren W. Nichols Donald G. Murphy ~i Contents Theoretic Mechanisms of in vitpo Senescence 1 F. MaPott Sinex . . . . . . . . . . . . Senescence in Ce1l Cu1ture: An Accumu1ation of Errors or Terminal Differentiation? 13 Vincent J. GPistofaZo . . . . . . . . . . . . . . This monograph, Senescence; Dominant or Recessive In Somatic Cell Crosses? represents the second annual workshop to promote theory and concept development in aging research. These workshops are part of a resource to bank cultured cells of special interest to aging research that was established at the Institute for Medical Research in Camden. New Jersey. by the National Institute on Aging in 1974. The underlying theme of the workshops is the use of cultured cells in a variety of somatic cell genetic systems designed to define mechanisms of in vitra cellular scenƯ escence and the possible insights that this may provide to the problems of in viva aging. The concept also includes bringing together workers from a variety of disciplines to stimulate new and innovative thoughts and work in the area. The current workƯ shop focuses on the relative role of nucleus and cytoplasm on determining the in vitra lifespan of human diploid cells as well as the relative influence of old and young cells when combined within a single cell structure. The techniques and procedures discussed should make significant contributions to understanding in vitra senescence and may lead to the mapping of an area or areas of the genome linked to senescence as is being accomplished with viral transformation of normal cells. Warren W. Nichols Donald G. Murphy ~i Contents Theoretic Mechanisms of in vitpo Senescence 1 F. MaPott Sinex ... ... Senescence in Ce1l Cu1ture: An Accumu1ation of Errors or Terminal Differentiation? 13 Vincent J. GPistofaZo ... ... Front Matter....Pages i-x Theoretical Mechanisms of in Vitro Senescence....Pages 1-11 Senescence in Cell Culture: An Accumulation of Errors or Terminal Differentiation?....Pages 13-21 Synkaryon and Heterokaryon Analyses of Clonal Senescence....Pages 23-38 Comments about the Paper by Norwood et al. and an Idea Relating Somatic Cell Mutations to Cellular Senescence....Pages 39-55 Fusion of Cytoplasts and Karyoplasts and How This Pertains to Research of Aging and Transformation....Pages 57-72 Dominance or Recessiveness of the Senescent Phenotype in Somatic Cell Fusions....Pages 73-79 Improved Techniques for the Fusion of Mammalian Cells....Pages 81-85 Mapping the SV40 Integration Sites in SV40-Transformed Human Cells....Pages 87-103 Discussion of Dr. Croce’s Paper....Pages 105-109 Research on Cellular Senescence: Present and Future Directions....Pages 111-120 Back Matter....Pages 121-123