Selected Topics In Mass Spectrometry In The Biomolecular Sciences (nato Science Series C:)

Many fundamental aspects of the methods used in mass spectrometry are here presented by outstanding scientists, with reference to very recent developments. The principles and applications of electrospray, ion spray and MALDI ionization technique are presented, together with optimised GC/MS interfacing systems and tools for quantitative analysis. A comprehensive treatment of modern instrumentation for mass analysis and detection is also included. The major part of the book deals with bioanalytical applications to peptides, proteins, oligonucleotides, polysaccharides, lipids and plant metabolites. Several papers are devoted to the evaluation of adduct formation between DNA and carcinogens. Environmental applications are also included, with examples of some specific cases. Fundamentals and applications are treated with the same degree of depth: the first two parts of the book therefore provide a basis for the understanding of the biomolecular applications section. \ __Audience:__ Ideal for advanced graduate students of chemistry who have learned some basic mass spectrometry. Also useful for Ph.D. students in chemistry, biology and medicine. Of value to researchers in academic and industrial laboratories. Front Matter....Pages i-xxvi Front Matter....Pages 1-1 Electrospray Ionization: Theory and Application....Pages 3-16 Atmospheric Pressure Ionization (ISI and APCI)....Pages 17-31 Matrix-Assisted Laser Desorption-Ionization (MALDI) Mass Spectrometry: Principles and Applications....Pages 33-53 High Sensitivity ESI MS and MALDI MS....Pages 55-84 Optimised GC/MS interfacing systems and operating conditions....Pages 85-101 Quantitative Analysis by Mass Spectrometry: Some Important Considerations....Pages 103-119 Optimization of Mass Spectrometric Ionisation Efficiency Data....Pages 121-127 Role of Internal Energy in Mass Spectrometric Fragmentation....Pages 129-142 Interaction of Metal Ions and Protons with Simple Biomolecules. Structure Information from Bond Energies and Entropy Effects....Pages 143-161 A Promising Marriage Between Theory and Experiment: Density Functional Method Versus Mass Spectrometry....Pages 163-179 Front Matter....Pages 181-181 Recent Advances in Magnet Sector Mass Spectrometry....Pages 183-211 Quadrupole Mass Filters and Quadrupole Ion Traps....Pages 213-238 Time-of-Flight Mass Spectrometry....Pages 239-262 Combined Analyzer Technologies....Pages 263-286 Fourier Transform Mass Spectrometry....Pages 287-314 Front Matter....Pages 315-315 Protein Characterization by MS in the Pharmaceutical Industry....Pages 317-345 Folding Pathways of Disulphide Containing Proteins....Pages 347-359 Integration of Chemical Modification Reactions and Mass Spectrometric Methods in Protein Characterisation....Pages 361-376 PSD-MALDI Analysis of Peptides....Pages 377-398 Development of a Mass Spectrometric Approach for the Characterisation of Hemoglobin Adducts....Pages 399-411 Front Matter....Pages 315-315 Reactivity of Antineoplastic Drugs with Model Peptides Studied by Advanced Mass Spectrometry Methodologies....Pages 413-425 Applications of Mass Spectrometry in Biochemical Studies of Nucleosides, Nucleotides and Nucleic Acids....Pages 427-453 MALDI Mass Analysis of Oligonucleotides....Pages 455-464 DNA Adducts in Human Carcinogenesis....Pages 465-476 MALDI MS of Oligo and Polysaccharides....Pages 477-499 Mass Spectrometry of Isoeicosanoids as Novel Lipid Substances....Pages 501-515 The Role of Mass Spectrometry in the Characterization of Plant Metabolites from Crude Extracts....Pages 517-531 Environmental Applications of Mass Spectrometry: Toxaphene Analysis....Pages 533-558 The Determination of Polar Compounds in the Aquatic Environment....Pages 559-573 Some Mass Spectrometry Contributions in the Study of Cultural Heritage....Pages 575-579 Back Matter....Pages 581-586 Many Fundamental Aspects Of The Methods Used In Mass Spectrometry Are Here Presented By Outstanding Scientists, With Reference To Very Recent Developments. The Principles And Applications Of Electrospray, Ion Spray And Maldi Ionization Technique Are Presented, Together With Optimised Gc/ms Interfacing Systems And Tools For Quantitative Analysis. A Comprehensive Treatment Of Modern Instrumentation For Mass Analysis And Detection Is Also Included. The Major Part Of The Book Deals With Bioanalytical Applications To Peptides, Proteins, Oligonucleotides, Polysaccharides, Lipids And Plant Metabolites. Several Papers Are Devoted To The Evaluation Of Adduct Formation Between Dna And Carcinogens. Environmental Applications Are Also Included, With Examples Of Some Specific Cases. Fundamentals And Applications Are Treated With The Same Degree Of Depth: The First Two Parts Of The Book Therefore Provide A Basis For The Understanding Of The Biomolecular Applications Section. Audience: Ideal For Advanced Graduate Students Of Chemistry Who Have Learned Some Basic Mass Spectrometry. Also Useful For Ph.d. Students In Chemistry, Biology And Medicine. Of Value To Researchers In Academic And Industrial Laboratories. Edited By Richard M. Caprioli, Antonio Malorni, Giovanni Sindona. Many fundamental aspects of the methods used in mass spectrometry are here presented by outstanding scientists, with reference to very recent developments. The principles and applications of electrospray, ion spray and MALDI ionization technique are presented, together with optimised GC/MS interfacing systems and tools for quantitative analysis. A comprehensive treatment of modern instrumentation for mass analysis and detection is also included. The major part of the book deals with bioanalytical applications to peptides, proteins, oligonucleotides, polysaccharides, lipids and plant metabolites. Several papers are devoted to the evaluation of adduct formation between DNA and carcinogens. Environmental applications are also included, with examples of some specific cases. Fundamentals and applications are treated with the same degree of depth: the first two parts of the book therefore provide a basis for the understanding of the biomolecular applications section. Audience: Ideal for advanced graduate students of chemistry who have learned some basic mass spectrometry. Also useful for Ph. D. students in chemistry, biology and medicine. Of value to researchers in academic and industrial laboratories