Reproduction Bundle: Human Preimplantation Embryo Selection (Reproductive Medicine and Assisted Reproductive Techniques Series) (Volume 5)

The most profound dilemma in assisted reproduction to date is the inability to recognize potentially viable embryos before their replacement into the reproductive tract. Application of increasingly advanced new technology has allowed the field of embryo evaluation to evolve rapidly and dramatically over the past five years. Human Preimplantation Embryo Selection assembles the leaders in the field of assisted reproduction to discuss their visions of dealing with the topic of embryo selection in the light of the limitations involving the fragility of the early human embryo. The purpose of this book is to shed light on the ever-expanding techniques that are being employed and evaluated with regard to embryo selection. A comprehensive overview may guide the ART practitioner in choice of techniques and evaluation of benefits and drawbacks. This book will provide an up-to-date overview and reference source: an invaluable tool for assisted reproductive practice worldwide. Front cover 1 Contents 6 Contributors 8 Preface 12 Acknowledgments 14 Chapter 1. Human oocyte and embryo assessment for ART 16 Chapter 2. The zona pellucida and markers of oocyte and embryo viability 30 Chapter 3. Morphology and kinetics of human pronuclei 36 Chapter 4. Human pronuclei as a mode of predicting viability 46 Chapter 5. Multinucleation and mosaicism in the human preimplantation embryo 56 Chapter 6. The origins and consequences of fragmentation in mammalian eggs and embryos 66 Chapter 7. Analysis of blastocyst morphology 94 Chapter 8. Morphometric analysis of human embryos 104 Chapter 9. Development rate, cumulative scoring, and embryonic viability 116 Chapter 10. Human embryo cryopreservation and its effects on embryo morphology 138 Chapter 11. Manipulating embryo development 150 Chapter 12. Assessment of soluble human leukocyte antigen G in human embryos 160 Chapter 13. Immunological aspects of embryo development 170 Chapter 14. Nitric oxide regulation of the preimplantation embryo 184 Chapter 15. Uptake and release of metabolites in human preimplantation embryos 194 Chapter 16. Preimplantation embryo metabolism and embryo interaction with the in vitro environment 206 Chapter 17. Polar body chromosome abnormalities and their consequences for human embryo development 216 Chapter 18. Chromosomal status of human embryos 224 Chapter 19. Genomic imprinting and consequences for embryonic development 250 Chapter 20. Selection of viable embryos and gametes by rapid, non-invasive metabolomic profiling of oxidative stress biomarkers 260 Chapter 21. Gene expression analysis in the human oocyte and embryo 278 Chapter 22. Mitochondria in reproduction: future assays for embryo selection 290 Chapter 23. Future genetic and other technologies for assessing embryos 302 Chapter 24. Oocyte selection in contemporary clinical IVF: do follicular markers of oocyte competence exist? 316 Chapter 25. Sperm DNA and embryo development 340 Chapter 26. The sperm centriole: its effect on the developing embryo 352 Index 370 Back cover 386