Regulation of Cell Proliferation and Differentiation (Cellular Senescence and Somatic Cell Genetics Book 1)

In 1974 The National Institute on Aging established a somatic cell genetic resource for aging research at the Institute for Medical Research in Camden, New Jersey. Within this program there is a yearly workshop to promote theory and concept develop­ ment in aging research with the specific purpose of addressing the use of genetically marked cells for aging research and to stimulate interest in aging research by workers in a variety of disciplines. This monograph, The Regulation of Cell Proliferation and Differentiation, is the result of the first workshop held May 15-17, 1975. The concept of the workshop was to consider two main areas: First, a discussion of clinical syndromes expressing as a major manifestation excessive growth, deficient growth or failure to thrive; and second, to present work in cellular and molecular biology on a model system suitable for in vitro study of regulation of cell proliferation and diff2rentiation. The model selected for this was skeletal muscle. It has been widely accepted that normal somatic cells from individual human donors display limited replicative lifespans when cultivated in vitro (1,2). That such "clonal senescence" may be related to in vivo aging is suggested by observations relating the replicative lifespans of cultures to donor age (3-5,13) donor genotype (4-7) and donor's tissue of origin (5,8). A variety of theories have been developed to explain in vitro clonal senescence (9). Front Matter....Pages i-xii Dysplasia, Malformations and Cancer, Especially with Respect to the Wiedemann-Beckwith Syndrome....Pages 1-64 Delayed Mutation as a Cause of Genetic Disease in Man: Achondroplasia and the Wiedemann-Beckwith Syndrome....Pages 65-79 Undergrowth Disorders....Pages 81-85 A Review of Controversial Issues in Myogenesis....Pages 87-104 The Culture Environment and Its Control of Myogenesis....Pages 105-137 Fusion, Phospholipase C, and Myogenesis....Pages 139-142 Application of Clonal Assay Methods to the Analysis of Tissue Development and Diseased States....Pages 143-164 Proliferative Growth during Myogenesis In Vitro ....Pages 165-176 Cell Fusion and Terminal Differentiation in Myogenic Cell Cultures....Pages 177-196 Back Matter....Pages 197-204