Redox Chemistry and Interfacial Behavior of Biological Molecules

The papers in this book were presented at the Third International Symposium on Redox Mechanisms and Interfacial Properties of Molecules of Biological Importance held in Honolulu, Hawaii between October 19-23, 1987. This Symposium was held as part of the 172nd Meeting of The Electrochemical Society which was cosponsored by The Electrochemical Society of Japan with the cooperation of The Japan Society of Applied Physics. The aim of the Symposium was to bring together a group of electrochemists and bio-medical scientists with interests in electrochemistry from around the world to present their most current research results and/or to present up-to-date reviews of current areas of research activity. It is quite clear from the diversity of topics covered in the various papers that electrochemistry and electrochemical techniques and principles have much to contribute to our under­ standing of many important biochemical phenomena. For example, electrochemical studies are providing important insights into the redox properties of biomolecules ranging from relatively small organic molecules such as indoleamine neurotransmitters to very large organic/organometallic molecules which include various redox enzymes or model enzyme systems. Many of the most powerful analytical techniques are now being coupled to electrodes to monitor potential-controlled behaviors of bio­ logical molecules at charged interfaces. Electrochemical techniques are now being developed which permit extraordinarily small electrodes to be inserted into single cells to monitor electroactive biomolecules. Other microelectrodes are being employed to control cell growth and to manipulate single cells. Front Matter....Pages i-xi Supramolecular Effects in the Redox and Coordination Chemistry of Superstructured Iron Porphyrins....Pages 1-25 Electrochemistry of Metalloporphyrins Containing A Metal-Metal or Metal-Carbon Bond....Pages 27-45 A Study of the Effects of Ionic Strength and Anion Binding on the Reduction Potential of Cytochrome c Using Microelectrodes....Pages 47-61 Electrochemical Reactivity of Strongly Adsorbed Cytochrome c....Pages 63-76 Redox Reactivity of Manganese “Capped” Metalloporphyrins....Pages 77-86 Metalloporphyrin Films on Solid Electrodes: Reflectance Spectroscopy and AC Impedance Studies in Aqueous and Nonaqueous Media....Pages 87-104 Bacterial Cytochrome P-450 Enzymes and Reactions on Immobilized Electrodes. I. Preliminary Studies....Pages 105-112 Interfacial Electrochemistry of Promoter Modified Electrodes for the Rapid Electron-Transfer of Cytochrome c....Pages 113-123 Sers Studies on the Electrode Reaction of Cytochrome c at Silver Electrode in the Presence of Pyridine Derivatives....Pages 125-138 The Electrochemical Characterization of the Oxene Adducts of PFe(ClO 4 ), PFe II , and PFe II (SR) [P = Tetrakis(2,6-Dichlorophenyl)Porphinato Dianion] in Acetonitrile: Models for Compounds I and II of Horseradish Peroxidase and for Activated Cytochrome P-450....Pages 139-149 Direct Electrical Communication between Chemically Modified Enzymes and Metal Electrodes: III. Electron-Transfer Relay Modified Glucose Oxidase and D-Amino-Acid Oxidase....Pages 151-171 Potential Controlled Enzymatic Activity of Conducting Enzyme Membrane....Pages 173-179 Electro-Enzymatic Studies of the Interfacial Behaviour of Proteins....Pages 181-191 Catalytic Oxidation of D-Glucose at an Enzyme-Modified Electrode with Entrapped Mediator....Pages 193-201 Electrochemistry of the Iron-Molybdenum Cofactor of Nitrogenase: Evidence for Multiple Speciation and Electrocatalytic Behavior....Pages 203-216 Serrs as a Probe of Electrode Processes: Interaction of Glucose Oxidase and Fad at Silver Electrodes....Pages 217-228 Surface Electrochemistry of Amino Acids: Voltammetry Assisted by Eels, Auger Spectroscopy and Leed....Pages 229-245 Electrochemistry and Spectroelectrochemistry of Adenine and Adenosine Complexes with 3d Transition Metals....Pages 247-266 Electrochemical Study of the Mechanism and Kinetics of Oxygen Reduction Mediated by Anthracycline Antibiotics Adsorbed on Electrode Surface....Pages 267-280 Studies on Redox Phototransformations of Reduced Forms of Coenzyme NADP + and Related Compounds....Pages 281-293 Anticancer Quinones and Quinolines: Mode of Action Via Electron Transfer and Oxidative Stress....Pages 295-307 Oxidation Pathways of Purine Drugs: Electrochemical, Liquid Chromatographic and Mass Spectrometric Insights....Pages 309-321 Electron Transfer Mechanism for Cocaine Action....Pages 323-331 Studies on Acid-Base, Hydration-Dehydration and Keto-Enol Equilibria in Aqueous Solutions of α-Ketoacids by Polarography, Linear Sweep Voltammetry and Spectroscopy....Pages 333-344 Electron Transfer-Oxy Radical Mechanism for Anticancer Agents: Etoposide, Cu Dips, and BIS(9-Aminoacridines)....Pages 345-357 Surface Enhanced Raman Scattering (SERS) Spectroscopy of Guanine Derivatives....Pages 359-367 Electrochemistry in Cancer: Applications to Pharmacological Studies of Quinone-Containing Antitumor Agents....Pages 369-382 On the Electrocatalytic Oxidation of D-Glucose and Related Polyols on Large Pt and Au Electrodes....Pages 383-395 Electrochemistry of Some Synthetic Oxygen Carrier Molecules in Water....Pages 397-406 Electrochemical Studies on the Generation of Active Oxygen Species in Biological Systems with the Use of Mediators....Pages 407-415 Intracellular Voltammetry with Ultrasmall Carbon Ring Electrodes....Pages 417-424 Oxidation Chemistry of CNS Indoles....Pages 425-445 Organic Electrochemistry, IV 1 : Electrochemical Desulfurization Reactions of Thiolumazines....Pages 447-455 Determination of Cationic Drugs on a Nafion-Coated Electrode....Pages 457-467 Adsorptive Stripping Voltammetric Studies of Biologically-Significant Compounds....Pages 469-478 Modified Electrodes used to Elucidate Electrochemical Behaviors of Biologically Significant Molecules....Pages 479-498 Studies of the Kinetics of Mediated Electrochemical Oxidation at Polymer-Coated Electrodes....Pages 499-514 Electrochemical Preparation of Metallophthalocyanine Films....Pages 515-520 Selective Determination of Phosphate with PhoE Porin-Lecithin Membrane Electrode....Pages 521-527 Membrane Bioenergetics as Viewed from Reconstitution Experiments....Pages 529-556 Surface Charge Determines the Aggregation of Hemoglobin Subunits as Predicted by the Surface Free Energy....Pages 557-564 Electrocatalysis in Membrane Mimetic Media....Pages 565-581 Some Techniques for Investigation of Electrochemical and Ion Transport Properties of Biological Cell Membranes: The Case of Chara Corallina....Pages 583-612 Vectorial Control of Cell Growth with Microelectrode....Pages 613-622 Electrochemical Manipulation of Single Cell with a Microelectrode....Pages 623-629 Introduction of new Alcohol Binding Sites at the Lipid/Water Interface by the Incorporation of Monosialogangliosides into DPPC Liposomes....Pages 631-640 Back Matter....Pages 641-659