Recombinant DNA Research and Viruses: Cloning and Expression of Viral Genes (Developments in Molecular Virology, 5)

The development of recombinant DNA technology has made a marked impact on molecular virology. The cleavage of viral DNA genomes with restriction enzymes and the cloning of such DNA fragments in bacterial p1asmids has led to the amplification of selected viral DNA fragments for sequencing and gene expression. RNA virus genomes which can be transcribed to their cDNA form were also cloned in bacterial p1asmids, facilitating the study of RNA virus genes. With the elucidation in recent years of the promoter sequence of various viral genes and the expression of these genes in bacteria or yeast, the understanding of many viral gene functions has made great progress. Cloning and expression of viral genes in mammalian cells was made possible by the construction of shuttle plasmid vectors which carry the origins of DNA replication from bacteria and/or mammalian viruses. The expression of viral genes in bacteria, yeast and eukaryotic cells gives reason to hope that it will be possible to produce viral antigens in large quantities for use as human or animal vaccines. The present volume attempts to capture for the reader some of the high­ lights of recombinant DNA research in the field of animal and plant viruses. Front Matter....Pages i-x Front Matter....Pages 1-1 Developments in Molecular Virology: Cloning of Retrovirus DNA in Bacteria and Cloning of other DNA in Retroviruses....Pages 3-14 Cloning of Human Oncogenes....Pages 15-35 Front Matter....Pages 37-37 Development of Plasmids and Cloning Procedures....Pages 39-57 Cloned DNA as a Substrate of Bacterial Recombination System....Pages 59-72 Utilization of λ Control Elements for Gene Expression Studies in Escherichia Coli ....Pages 73-86 Front Matter....Pages 87-87 Cloning and Mapping of African Swine Fever Virus DNA....Pages 89-102 Cloning of the DNA of Alphaherpesvirinae....Pages 103-124 The Cloning and Sequencing of Sites of Linkage between Adenovirus DNA and Cellular DNA: Recombination of Foreign DNA with the Mammalian Genome....Pages 125-171 Cloning of Papillomavirus DNA....Pages 173-197 Front Matter....Pages 199-199 Cloning of Bovine Leukemia Virus Proviral Information....Pages 201-204 Cloning of Endogenous ‘Retrovirus-Like’ Genes: The Murine VL30 Family.....Pages 205-220 Front Matter....Pages 221-221 Poliovirus cDNA Cloned in Bacterial Plasmids....Pages 223-244 Front Matter....Pages 245-245 Cloning and Manipulating Cauliflower Mosaic Virus....Pages 247-275 Cloning of Plant-Virus Genomes Other than that of Cauliflower Mosaic Virus....Pages 277-292 Cloning of Viroid cDNA....Pages 293-304 Front Matter....Pages 305-305 Expression of Cloned Genes Under Phage λ Control....Pages 307-326 Expression of Herpes Simplex Virus Type 1 and Type 2 Glycoprotein D Genes Using the Escherichia Coli lac Promoter....Pages 327-352 Cloning and Expression of Foot and Mouth Disease Virus Genes....Pages 353-366 Back Matter....Pages 367-373