Receptor Purification : Volume 1 Receptors for CNS Agents, Growth Factors, Hormones, and Related Substances

The purpose of these volumes is to provide a reference work for the methods of purifying many of the receptors we know about. This be­ comes increasingly important as full-length receptors are overexpressed in bacteria or in insect cell systems. A major problem for abundantly expressed proteins will be their purification. In addition to purification protocols, many other details can be found concerning an individual receptor that may not be available in standard texts or monographs. No book of this type is available as a compendium of purification procedures. Receptor Purification provides protocols for the purification of a wide variety of receptors. These include receptors that bind: neurotransmit­ ters, polypeptide hormones, steroid hormones, and ligands for related members of the steroid supergene family and others, including receptors involved in bacterial motion. The text of this information is substantial, so as to require its publication in two volumes. Consequently, a division was made by grouping receptors by the nature of their ligands. Thus, in Volume One there are contributions on serotonin receptors, adrenergic receptors, the purification of GTP-binding proteins, opioid receptors, neurotensin receptor, luteinizing hormone receptor, human chorionic gonadotropin receptor, follicle stimulating hormone receptor, thyro­ tropin receptor, prolactin receptor, epidermal growth factor receptor, platelet derived growth factor receptor, colony stimulating factor recep­ tor, insulin-like growth factor receptors, insulin receptor, fibronectin receptor, interferon receptor, and the cholecystokinin receptor. Front Matter....Pages i-xiii Serotonin Receptors....Pages 1-21 Purification and Cloning of Central Serotonin Receptors....Pages 23-43 Purification of β-Adrenergic Receptors....Pages 45-75 Purification of β,γ Subunits of Guanine Nucleotide Binding Proteins....Pages 77-98 Purification of Opioid Binding Proteins....Pages 99-113 μ-Opioid Receptor....Pages 115-129 The Neurotensin Receptor from Mammalian Brain....Pages 131-145 The Luteinizing Hormone/Human Chorionic Gonadotropin Receptor of Testis and Ovary....Pages 147-171 Purification of LH/hCG Receptor....Pages 173-190 The Follicle Stimulating Hormone Receptor....Pages 191-215 Thyrotropin Receptor....Pages 217-248 Thyrotropin Receptor Purification....Pages 249-268 Prolactin Receptors....Pages 269-288 Ligand- and Antibody-Affinity Purification of the Epidermal Growth Factor Receptor....Pages 289-302 Platelet-Derived Growth Factor B Type Receptor....Pages 303-314 Colony-Stimulating Factor 1 Receptor....Pages 315-328 Insulin-Like Growth Factor-II Receptors....Pages 329-346 Purification of Insulin and Insulin-Like Growth Factor (IGF)-I Receptor....Pages 347-371 Insulin Receptor Isolation Through Avidin-Biotin Technology....Pages 373-402 Insulin Receptor Purification....Pages 403-417 Insulin Receptor Purification....Pages 419-433 Isolation of Fibronectin Receptors....Pages 435-449 Receptor Purification Using the Complementary Hydropathy Approach....Pages 451-457 Purification of the Human Interferon-γ Receptor by Ligand Affinity....Pages 459-481 Purification of the Cholecystokinin Receptor....Pages 483-495 Back Matter....Pages 497-501 A two-volume guide to the purification of most receptor types, including receptors for neurotransmitters, polypeptide hormones, steroid hormones, and a variety of other ligands involved in many biological processes. It provides a comprehensive source of techniques to enable the purification of cloned receptors.