Recent Advances in Virus Diagnosis : A Seminar in the CEC Programme of Co-ordination of Research on Animal Pathology, Held at the Veterinary Research Laboratories, Belfast, Northern Ireland, September 22–23, 1983

The traditional approach to diagnosis of virus infectians by isolation of the causative virus is usually both slow and expensive. More recently, the emphasis has been on the direct detection of viruses or viral antigens in clinical specimens. This can be done using established techniques such as immunofluorescence or electron microscopy, or by newly developed biochemical methods. The purpose of this meeting was to review these and other developments in the laboratory diagnosis of virus infections. We would like to thank all those who contributed to the success of this meeting. In particular, we are grateful to the CEC for financial sponsorship, to Professor C. Dow, Director of the Veterinary Research Laboratories, for help in organising the meeting, and to Miss B. Hamilton for her excellent typing of the manuscripts. M S McNulty J B McFerran VII CONTENTS Page Preface V VIRAL DIAGNOSIS BY TIMMUNOFLUORESCENCE P. S. Gardner APPLICATIONS OF IMMUNOFLUORESCENCE IN VETERINARY VIRAL DIAGNOSIS M. S. McNulty and G. M. Allan 15 TAGGING OF VIRAL ANTIGENS USING IMMUNOPEROXIDASE . AND IMMUNOOOLD TEGINlQUES R. Ducatelle, F. Castryck and J. Hoorens 27 DETECTION OF BVD VIRUS IN VlRAEMIC CATTLE BY AN INDIRECT IMMUNOPEROXIDASE TEGINIQUE A. Meyling 37 VIRAL DIAGNOSIS BY ELECTRON MICROSCOPY J. D. Almeida 47 CAPTURE ASSAYS FOR THE DETECTION OF VIRUS-SPECIFIC IgM ANTIBODY R. S. Tedder 60 DETECTION . AND IDENTIFICATION OF PAPILLOMAVIRUSES IN BENIGN . AND MALIGNANT 'TIlmJRS OF CATTLE M. S. Campo 72 DETECTION OF VIRAL NUCLEIC ACIDS IN CELL CULTURES . Front Matter....Pages I-VIII Viral Diagnosis by Immunofluorescence....Pages 1-14 Applications of Immunofluorescence in Veterinary Viral Diagnosis....Pages 15-26 Tagging of Viral Antigens using Immunoperoxidase and Immunogold Techniques....Pages 27-36 Detection of BVD Virus in Viremic Cattle by an Indirect Immunoperoxidase Technique....Pages 37-46 Viral Diagnosis by Electron Microscopy....Pages 47-59 Capture Assays for the Detection of Virus-Specific IgM Antibody....Pages 60-71 Detection and Identification of Papillomaviruses in Benign and Malignant Tumours of Cattle....Pages 72-94 Detection of Viral Nucleic Acids in Cell Cultures and in Clinical Specimens by Spot Hybridization....Pages 95-100 Restriction Endonuclease Analysis of Aujeszky’s Disease Virus DNA....Pages 101-114 Protein Blotting: The Basic Method and its Role in Viral Diagnosis....Pages 115-124 The Use of Polyacrylamide Gel Electrophoresis of Virus RNA in the Study of Rotavirus Infections....Pages 125-135 Examination of Rotavirus from Bovine and Porcine Species by Western Blotting....Pages 136-144 Use of Monoclonal Antibodies in Virus Diagnosis....Pages 145-156 Bovine Leukosis — Recent Developments with use of Monoclonal Antibodies and Elisa Tests....Pages 157-170 Radioimmunoassay....Pages 171-185 Immunoelectrophoretic Techniques....Pages 186-194 Diagnostic use of CNS Antibodies....Pages 195-210 Back Matter....Pages 211-213