Pyridine Nucleotide-Dependent Dehydrogenases : Proceedings of an Advanced Study Institute Held at the University of Konstanz, Germany, September 15–20, 1969

The present volume represents the proceedings of the symposium on npyridine Nucleotide-Dependent Dehydrogenases" which was held on the campus of the re­ cently established University of Konstanz, Germany, from September 15 to 20, 1969. The aim of the symposium was to provide a forum for discussion among the experts interested in the various aspects of pyridine nucleotide-dependent de­ hydrogenases and the pyridine coenzymes, so as to evaluate the state of the present knowledge and to stimulate further progress in this field. In order to facilitate discussion and personal contact it was necessary to restrict the number of participants to 90 including the invited speakers, who came from fifteen countries (Australia, Austria, Denmark, England, Finland, France, Germany, Hungary, Israel, Italy, Japan, Netherlands, Sweden, Switzerland, USA). The printed lectures in this volume differ only in minor respects from those circulated be fore the meeting. In some cases speakers presented new experimen­ tal material during the symposium which was added as an addendum to the papers. The discussions were not recorded. The participants were requested to provide a written report of what they considered worth including in the published re­ port. This, together with the answers of the speakers is presented in this vo­ lume. I whish to thank all the speakers and discussants for their cooperativity in preparing the manuscript. The symposium was sponsored by the International Union of Biochemistry whose President, Professor Theorell, was also present and who was acting as one of the speakers and chairmen. Front Matter....Pages I-XV Opening Remarks....Pages 1-2 Kinetics....Pages 3-13 The Mechanism of Hydride Transfer....Pages 15-29 The Significance of the Investigation of Model Compounds for the Elucidation of the Mechanism of Hydrogen-Transfer with Pyridine Nucleotides....Pages 31-38 The Structure of Pyridine Coenzymes as Related to Binding....Pages 39-56 The Primary Structure and Activity of Glyceraldehyde 3-Phosphate Dehydrogenase....Pages 57-70 Quaternary Structure and Conformation of Lactic Dehydrogenase and Glyceraldehyde-3-Phosphate Dehydrogenase....Pages 71-90 The Stereoselective Inhibition of Functional -SH Groups of Dehydrogenases....Pages 91-101 The Role of Zinc Ions, -SH Groups, and Histidyl Residues in the Mechanism of Dehydrogenases....Pages 103-120 Structural and Functional Relationships between Isoenzymes of Horse LADH....Pages 121-128 X-Ray Studies of Horse Liver Alcohol Dehydrogenase....Pages 129-134 Lactate Dehydrogenase....Pages 135-143 Studies on Structure and Active Sites of Lactate Dehydrogenase from Pig Heart and Pig Muscle....Pages 145-156 Structure and Mechanism of Lactate Dehydrogenase....Pages 157-174 Selective Reactivity of Functional Groups in Glyceraldehyde 3-Phosphate Dehydrogenase....Pages 175-186 On the Relationship between Protein Conformation and Enzyme-Substrate Covalent Bond Formation in Glyceraldehyde-3-Phosphate Dehydrogenase....Pages 187-197 Cooperative Phenomena in Yeast Glyceraldehyde-3-Phosphate Dehydrogenase....Pages 199-208 Conformational Effects of NAD + on Yeast Glyceraldehyde-3-Phosphate Dehydrogenase....Pages 209-216 Recent Studies on the Allosteric Glyceraldehyde-3-Phosphate Dehydrogenase from Yeast....Pages 217-228 Subunit Interactions in Glyceraldehyde-3-Phosphate Dehydrogenase: A Fluorometric and Calorimetric Analysis of DPN Binding as a Function of Temperature....Pages 229-232 Muscle Glyceraldehydephosphate Dehydrogenase: NAD + Binding and its Implications for the Mechanism of Action of the Enzyme....Pages 233-244 Kinetics and Mechanism of Glutamate Dehydrogenase....Pages 245-256 Optical Probes for Glutamate Dehydrogenase....Pages 257-269 Glutamate Dehydrogenase — A Study on its Inactivation....Pages 271-278 Quaternary Structure and Enzymic Properties of Beef Liver Glutamate Dehydrogenase....Pages 279-292 Structure and Association of Glutamate Dehydrogenase Solutions....Pages 293-304 Mechanism of Action of Glutamate Dehydrogenase from Various Sources....Pages 305-314 Kinetic Studies of NADP-Dependent Isocitrate Dehydrogenase from Beef Heart Mitochondria....Pages 315-324 The Role of NAD-Linked Dehydrogenases in the Biosynthesis of UDP-D-Xylose....Pages 325-333 Regulation by NADP + and NADPH of Transhydrogenase from Azotobacter Vinelandii....Pages 335-349 Activation Effect of 2′-Adenylic Acid on Bacterial Transhydrogenases....Pages 351-357 The Role of NAD + in the Catalytic Mechanism of Lipoamide Dehydrogenase....Pages 359-373 The NADH Dehydrogenase of the Respiratory Chain....Pages 375-391 A New Intermediate in TPNH-Linked Flavoproteins....Pages 393-411 Regulation of the Redox State of the Pyridine Nucleotides in Rat Liver....Pages 413-438 The State of the DPN System in Liver. An Analysis of Pyridine Nucleotide Levels, Surface Fluorescence, and Redox Potentials of Indicator Metabolite Couples in the Hemoglobin-Free Perfused Rat Liver....Pages 439-461 Closing Remarks....Pages 463-464 Back Matter....Pages 465-472