Proteins: Structure, Function, and Engineering

Here, researchers review the latest breakthroughs in protein research. Their contributions explore emerging principles and techniques and survey important classes of proteins that will play key roles in the field's future. Articles examine the possibility of a Boltzman-like distribution in protein substructures, the new technique of Raman spectroscopy, and compact intermediate states of protein folding. This well-illustrated volume also features coverage of proteins that bind nucleic acids. Front Matter....Pages i-xx Boltzmann-like Statistics of Protein Architectures....Pages 1-26 Compact Intermediates States in Protein Folding....Pages 27-53 Raman Spectroscopy of Proteins and Their Assemblies....Pages 55-99 Fluorescence Spectroscopic Studies of Proteins....Pages 101-114 Pyrene Excimer Fluorescence as a Probe of Protein Conformational Change....Pages 115-132 Differential Scanning Calorimetry of Proteins....Pages 133-176 Affinity Labeling and Related Approaches to Targeting Specific Enzyme Sites....Pages 177-205 Tryptophan Synthase....Pages 207-254 Structure and Function in the Tubulin Dimer and the Role of the Acidic Carboxyl Terminus....Pages 255-302 Role of Allosteric Changes in Cyclic AMP Receptor Protein Function....Pages 303-321 A Family of RNA-Binding Enzymes....Pages 323-376 Functions and Structures of Ribonuclease H Enzymes....Pages 377-422 Back Matter....Pages 423-436