Proteins Involved in DNA Replication (Advances in Experimental Medicine and Biology, 179)

This book collects the Proceedings of a workshop sponsored by the European Molecular Biology Organization (EMBO) entitled "Pro­ teins Involved in DNA Replication" which was held September 19 to 23,1983 at Vitznau, near Lucerne, in Switzerland. The aim of this workshop was to review and discuss the status of our knowledge on the intricate array of enzymes and proteins that allow the replication of the DNA. Since the first discovery of a DNA polymerase in Escherichia coli by Arthur Kornberg twenty eight years ago, a great number of enzymes and other proteins were des­ cribed that are essential for this process: different DNA poly­ merases, DNA primases, DNA dependent ATPases, helicases, DNA liga­ ses, DNA topoisomerases, exo- and endonucleases, DNA binding pro­ teins and others. They are required for the initiation of a round of synthesis at each replication origin, for the progress of the growing fork, for the disentanglement of the replication product, or for assuring the fidelity of the replication process. The number, variety and ways in which these proteins inter­ act with DNA and with each other to the achievement of replication and to the maintenance of the physiological structure of the chromo­ somes is the subject of the contributions collected in this volume. The presentations and discussions during this workshop reinforced the view that DNA replication in vivo can only be achieved through the cooperation of a high number of enzymes, proteins and other cofactors. Front Matter....Pages i-xii Front Matter....Pages 1-1 Enzyme Studies of Replication of the Escherichia Coli Chromosome....Pages 3-16 Enzymological Studies of the T4 Replication Proteins....Pages 17-33 In Vitro Replication of Bacteriophage Φ29....Pages 35-44 Proteins and Nucleotide Sequences Involved in DNA Replication of Filamentous Bacteriophage....Pages 45-54 In Vitro Replication of Rl Miniplasmid DNA....Pages 55-61 Analysis of Mu DNA Replicated on Cellophane Discs....Pages 63-67 Replication of Bacteriophage Mu and its Mini-Mu Derivatives....Pages 69-76 Initiation of DNA Synthesis on Single-Stranded DNA Templates in Vitro Promoted by the Bacteriophage λ O and P Replication Proteins....Pages 77-89 Front Matter....Pages 91-91 Replication in Vitro of Adenovirus DNA....Pages 93-105 Characterization of in Vitro DNA Synthesis in an Isolated Chloroplast System of Petunia Hybrida....Pages 107-112 In Vitro Synthesis of Cauliflower Mosaic Virus DNA in Viroplasms....Pages 113-119 Search for the Enzyme Responsible for the Reverse Transcription Step in Cauliflower Mosaic Virus Replication....Pages 121-125 Chromatin Structure and DNA Replication....Pages 127-141 Inhibition and Recovery of the Replication of Depurinated Parvovirus DNA in Mouse Fibroblasts....Pages 143-150 The Genetics of Adeno-Associated Virus....Pages 151-161 Relationship between the Organization of DNA Loop Domains and of Replicons in the Eukaryotic Genome....Pages 163-168 Aphidicolin and Eukaryotic DNA Synthesis....Pages 169-181 Front Matter....Pages 183-183 The Origin of DNA Replication of Bacteriophage fl and its Interaction with the Phage Gene II Protein....Pages 185-191 Overproduction and Purification of the Gene 2 Product Involved in the Initiation of Phage Φ29 Replication....Pages 193-197 Autorepression of the dnaA Gene of Escherichia Coli....Pages 199-207 Front Matter....Pages 183-183 Replication Functions Encoded by the Plasmid pSClOl....Pages 209-214 How Does Rop Work?....Pages 215-219 Gene A Protein of Bacteriophage ΦX174 is a Highly Specific Single-Strand Nuclease and Binds Via a Tyrosyl Residue to DNA After Cleavage....Pages 221-230 Gene a Protein Interacting with Recombinant Plasmid DNAs Containing 25–30 b.p. of the ΦX174 Replication Origin....Pages 231-240 How Does SV40 T Antigen Control Initiation of Viral DNA Replication?....Pages 241-248 Studies on the Initiation of DNA Synthesis in Plant and Animal Cells....Pages 249-262 Front Matter....Pages 263-263 Function and Properties of RP4 DNA Primase....Pages 265-280 De Novo DNA Synthesis by Yeast DNA Polymerase I Associated with Primase Activity....Pages 281-286 Paradoxes of in Situ Polyacrylamide Gel Assays for DNA Polymerase Priming....Pages 287-293 Association between Primase and DNA Polymerase α in Murine Cells....Pages 295-305 Properties of the Primase Activity of the 9 S DNA Polymerase α from Calf Thymus....Pages 307-311 Front Matter....Pages 313-313 DNA Polymerase III Holoenzyme of Escherichia Coli : An Asymmetric Dimeric Replicative Complex Containing Distinguishable Leading and Lagging Strand Polymerases....Pages 315-319 Mammalian DNA Polymerase α Holoenzyme....Pages 321-330 DNA Polymerase Activities of Mammalian Cell-Cycle Mutants and “Wild-Type” Cells in Different States of Proliferative Activity and Quiescence....Pages 331-335 Correlation of DNA Polymerase Activities with the Initiation of DNA Synthesis....Pages 337-342 Synthesis of Catalytically Active Polymerase α by in Vitro Translation of Calf RNA....Pages 343-353 Interaction of DNA Accessory Proteins with DNA Polymerase β of the Novikoff Hepatoma....Pages 355-361 Plasmid Cloning of DNA Polymerase I in Escherichia Coli and Saccharomyces Cerevisiae ....Pages 363-372 Structural and Functional Properties of DNA Polymerase α from Calf Thymus....Pages 373-381 Front Matter....Pages 383-383 Functions of DNA Helicases in the DNA Metabolism of Escherichia Coli ....Pages 385-393 Front Matter....Pages 383-383 Escherichia coli DNA Gyrase....Pages 395-407 A Unique ATP-Dependent DNA Topoisomerase from Trypanosomatids....Pages 409-422 Regenerating Rat Liver Topoisomerase II: Purification of the Enzyme and Catenation of DNA Rings....Pages 423-433 Expression of Silent Genes: Possible Interaction between DNA Gyrase and RNA Polymerase....Pages 435-440 Poly (ADP-Ribosylation) and DNA Topoisomerase I in Different Cell Lines....Pages 441-447 Effect of UV Induced DNA Lesions on the Activity of Escherichia Coli DNA Topoisomerases: A Possible Role of these Enzymes in DNA Repair....Pages 449-454 Front Matter....Pages 455-455 The Structure of the Bacterial Nucleoid....Pages 457-466 Proteins from the Prokaryotic Nucleoid: Biochemical and 1 H NMR Studies on Three Bacterial Histone-Like Proteins....Pages 467-477 The Role of HMG1 Protein in Nucleosome Assembly and in Chromatin Replication....Pages 479-488 DNA-Binding Proteins in Replicating and Mitotically Arrested Brain Neurons....Pages 489-495 Searching for Proteins and Sequences of DNA Replication in Mammalian Cells....Pages 497-506 Diadenosine Tetraphosphate and Diadenosine Tetraphosphate-Binding Proteins in Developing Embryos of Artemia....Pages 507-511 A Ribonuclease H from Yeast Stimulates DNA Polymerase in Vitro....Pages 513-518 Catalytic Activities of Human Poly (ADP-Ribose) Polymerase after SDS-Page....Pages 519-522 Front Matter....Pages 523-523 Fidelity of DNA Replication in Vitro....Pages 525-533 Replication of ΦX174 DNA by Calf Thymus DNA Polymerase-α: Measurement of Error Rates at the Amber-16 Codon....Pages 535-540 Front Matter....Pages 541-541 Studies on the Role of dam Methylation at the Escherichia Coli Chromosome Replication Origin ( oriC )....Pages 543-549 DNA Methylation and DNA Structure....Pages 551-556 Back Matter....Pages 557-571 This book collects the Proceedings of a workshop sponsored by the European Molecular Biology Organization (EMBO) entitled "ProƯ teins Involved in DNA Replication" which was held September 19 to 23,1983 at Vitznau, near Lucerne, in Switzerland. The aim of this workshop was to review and discuss the status of our knowledge on the intricate array of enzymes and proteins that allow the replication of the DNA. Since the first discovery of a DNA polymerase in Escherichia coli by Arthur Kornberg twenty eight years ago, a great number of enzymes and other proteins were desƯ cribed that are essential for this process: different DNA polyƯ merases, DNA primases, DNA dependent ATPases, helicases, DNA ligaƯ ses, DNA topoisomerases, exo- and endonucleases, DNA binding proƯ teins and others. They are required for the initiation of a round of synthesis at each replication origin, for the progress of the growing fork, for the disentanglement of the replication product, or for assuring the fidelity of the replication process. The number, variety and ways in which these proteins interƯ act with DNA and with each other to the achievement of replication and to the maintenance of the physiological structure of the chromoƯ somes is the subject of the contributions collected in this volume. The presentations and discussions during this workshop reinforced the view that DNA replication in vivo can only be achieved through the cooperation of a high number of enzymes, proteins and other cofactors