Protein Misfolding And Cellular Stress In Disease And Aging: Concepts And Protocols (methods In Molecular Biology)

How and why certain proteins misfold and how this misfolding is linked to many disease processes has become a well-documented topic of study. Protein Misfolding and Cellular Stress in Disease and Aging: Concepts and Protocols moves beyond the basics to emphasize the molecular effects of protein misfolding at a cellular level, to delineate the impacts and cellular reactions that play a role in pathogenetic mechanisms, and to pinpoint possible manipulations and treatment strategies that can counteract, modify, or delay the consequences of misfolding. The volume begins with several concepts and approaches developed in the recent past including a connection to the research field of aging, where protein misfolding diseases have been equated to premature aging processes, and the book’s coverage continues with detailed descriptions of protocols for relevant experimental approaches. Written in the highly successful Methods in Molecular BiologyTM series format, protocols chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Misfolding and Cellular Stress in Disease and Aging: Concepts and Protocols aims to aid researchers in the field, as well as medical professionals and molecular biologists, in shaping and performing research related to this intriguing and vital subject. Protein misfolding and celluar stress : an overview / Niels Gregersen and Peter Bross Protein aggregation diseases : toxicity of soluble prefibrillar aggregates and their clinical significance / Massimo Stefani Consequences of stress in the secretary pathway : the ER stress response and its role in the metabolic syndrome / Martin Schröder and Louise Sutcliffe What role does mitochondrial stress play in neurodegenerative diseases? / Alicia Mae Pickrell and Carlos Torres Moraes Autophagy in disease / Dalibor Mijaljica, Mark Prescott, and Rodney J. Devenish Mitophagy and mitoptosis in disease processes / Dalibor Mijaljica, Mark Prescott, and Rodney J. Devenish Cellular stress and protein misfolding during aging / Rajiv Vaid Basaiawmoit and Suresh I.S. Rattan Measuring consequences of protein misfolding and cellular stress using OMICS techniques / Peter Bross ... [et al.] Production of cells with targeted integration of gene variants of human ABC transporter for stable and regulated expression using the Flp recombinase system / Kanako Wakabayashi-Nakao ... [et al.] Stress response profile in human fribroblasts exposed to heat shock or oxidative stress / Christina Bak Pedersen and Niels Gregersen Examining ubiquitinated protein aggregates in tissue sections / Natalia A. Kaniuk and John H. Brumell Determination of proteasomal activities / Cristovao F. Lima and Suresh I.S. Rattan Measurement of autophagy in cells and tissues / Isei Tanida and Satoshi Waguri Transcription factor sequestration by polyglutamine proteins / Tomoyuki Yamanaka and Nobuyuki Nukina Biological membranes as protein aggregation matrices and targets of amyloid toxicity / Monica Bucciantini and Cristina Cecchi Measurement of mitochondrial ROS production / Anatoly A. Starkov In vivo detection of oxidized proteins : a practical approach to tissue-derived mitochondria / Francesca Maltecca and Giorgio Casari Measurement of oxidized/reduced glutathione ratio / Joshua B. Owen and D. Allan Butterfield Determination of altered mitochondria ultrastructure by electron microscopy / Shoichi Sasaki Assessing bad sub-cellular localization under conditions associated with prevention or promotion of mitochondrial permeability transition-dependent toxicity / Liana Cerioni and Orazio Cantoni A cellular viability assay to monitor drug toxicity / Jakob Hansen and Peter Bross A cellular viability assay to monitor drug toxicity / Jakob Hansen and Peter Bross Rescue of misfolded proteins and stabilization by small molecules / Raymond C. Stevens, Javier Sancho, and Aurora Martinez. Front Matter....Pages i-xi Front Matter....Pages 1-1 Front Matter....Pages 3-23 Front Matter....Pages 25-41 Back Matter....Pages 43-62 ....Pages 63-78