Protein Arrays, Biochips and Proteomics: The Next Phase of Genomic Discovery (No Series)

From disease marker identification to accelerated drug development, this reference offers a detailed overview of current and emerging trends in the field of proteomics—focusing on innovations in protein microarrays and biochips, mass spectrometry, high-throughput protein expression, protein–protein interactions, structural proteomics, and the proteomic marketplace for comprehensive understanding of proteomic research in the past, present, and future. Team DDU......Page 1 Foreword Sam Hanash......Page 4 Preface......Page 6 Contents......Page 10 Contributors......Page 12 1. Protein Biochips and Array-Based Proteomics......Page 16 2. Ultrasensitive Microarray-Based Ligand Assay Technology......Page 96 3. Practical Approaches to Protein Microarrays......Page 142 4. Protein Biochips: Powerful New Tools to Unravel the Complexity of Proteomics?......Page 160 5. Functionalized Surfaces for Protein Microarrays: State of the Art, Challenges, and Perspectives......Page 174 6. High-Throughput Protein Expression, Purification, and Characterization Technologies......Page 188 7. Miniaturized Protein Production for Proteomics......Page 218 8. Protein Profiling: Proteomes and Subproteomes......Page 232 9. Shotgun Proteomics and Its Applications to the Yeast Proteome......Page 248 10. Forward and Reverse Proteomics: It Takes Two (or More) to Tango......Page 270 11. Dynamic Visualization of Expressed Gene Networks......Page 292 12. High-Throughput Structural Biology and Proteomics......Page 314 13. Integration of Proteomic, Genechip, and DNA Sequence Data......Page 340 14. The Proteomics Market......Page 352 Index......Page 366 From disease marker identification to accelerated drug development, Protein Arrays, Biochips, and Proteomics offers a detailed overview of current and emerging trends in the field of array-based proteomics. This reference focuses on innovations in protein microarrays and biochips, mass spectrometry, high-throughput protein expression, protein-protein interactions, structural proteomics, and the proteomic marketplace for comprehensive understanding of past, present, and future proteomic research. Offering an abundance of figures and charts, the book compiles a wide variety of technologies and applications ranging from functionalized chip surfaces to strategies for protein expression.
Offering an abundance of figures and charts, this book considers the generation and development of new protein array technologies; the exploration of cellular networks in efficient, effective, and parallelized formats; theoretical and practical aspects of protein diagnostics and therapeutics; the integration of genomics and proteomics information; and the utilization of expressed sequence tags, cDNA databases, and robotics for recombinant protein expression and recovery. From disease marker identification to accelerated drug development, this book offers an overview of the trends in the field of array-based proteomics. It compiles a wide variety of technologies and applications ranging from functionalized chip surfaces to strategies for protein expression.