Protein Arrays, Biochips and Proteomics: The Next Phase of Genomic Discovery (No Series)

From disease marker identification to accelerated drug development, Protein Arrays, Biochips, and Proteomics offers a detailed overview of current and emerging trends in the field of array-based proteomics. This reference focuses on innovations in protein microarrays and biochips, mass spectrometry, high-throughput protein expression, protein-protein interactions, structural proteomics, and the proteomic marketplace for comprehensive understanding of past, present, and future proteomic research. Offering an abundance of figures and charts, the book compiles a wide variety of technologies and applications ranging from functionalized chip surfaces to strategies for protein expression. Team DDU Protein Arrays, Biochips, and Proteomics 1 Cover 1 Foreword Sam Hanash 4 Preface 6 Contents 10 Contributors 12 1. Protein Biochips and Array-Based Proteomics 16 2. Ultrasensitive Microarray-Based Ligand Assay Technology 96 3. Practical Approaches to Protein Microarrays 142 4. Protein Biochips: Powerful New Tools to Unravel the Complexity of Proteomics? 160 5. Functionalized Surfaces for Protein Microarrays: State of the Art, Challenges, and Perspectives 174 6. High-Throughput Protein Expression, Purification, and Characterization Technologies 188 7. Miniaturized Protein Production for Proteomics 218 8. Protein Profiling: Proteomes and Subproteomes 232 9. Shotgun Proteomics and Its Applications to the Yeast Proteome 248 10. Forward and Reverse Proteomics: It Takes Two (or More) to Tango 270 11. Dynamic Visualization of Expressed Gene Networks 292 12. High-Throughput Structural Biology and Proteomics 314 13. Integration of Proteomic, Genechip, and DNA Sequence Data 340 14. The Proteomics Market 352 Index 366 Team DDU 1 Offering an abundance of figures and charts, this book considers the generation and development of new protein array technologies; the exploration of cellular networks in efficient, effective, and parallelized formats; theoretical and practical aspects of protein diagnostics and therapeutics; the integration of genomics and proteomics information; and the utilization of expressed sequence tags, cDNA databases, and robotics for recombinant protein expression and recovery. From disease marker identification to accelerated drug development, this reference offers a detailed overview of current and emerging trends in the field of proteomics—focusing on innovations in protein microarrays and biochips, mass spectrometry, high-throughput protein expression, protein–protein interactions, structural proteomics, and the proteomic marketplace for comprehensive understanding of proteomic research in the past, present, and future.

From disease marker identification to accelerated drug development, Protein Arrays, Biochips, and Proteomics offers a detailed overview of current and emerging trends in the field of array-based proteomics. This reference focuses on innovations in protein microarrays and biochips, mass spectrometry, high-throughput protein expression, protein-prote

From disease marker identification to accelerated drug development, this reference offers a detailed overview of trends in the field of proteomics, including innovations in protein microarrays and biochips, mass spectrometry, high-throughput protein expression, and protein-protein interactions From disease marker identification to accelerated drug development, this book offers an overview of the trends in the field of array-based proteomics. It compiles a wide variety of technologies and applications ranging from functionalized chip surfaces to strategies for protein expression.