Protein Aggregation in Bacteria: Functional and Structural Properties of Inclusion Bodies in Bacterial Cells (Wiley Series in Protein and Peptide Science)

Focuses on the aggregation of recombinant proteins in bacterial cells in the form of inclusion bodies--and on their use in biotechnological and medical applications The first book devoted specifically to the topic of aggregation in bacteria, ''Protein Aggregation in Bacteria: Functional and Structural Properties of Inclusion Bodies in Bacterial Cells'' provides a large overview of protein folding and aggregation, including cell biology and methodological aspects. It summarizes, for the first time in one book, ideas and technical approaches that pave the way for a direct use of inclusion bodies in biotechnological and medical applications. ''Protein Aggregation in Bacteria'' covers: Molecular and cellular mechanisms of protein folding, aggregation, and disaggregation in bacteria Physiological importance and consequences of aggregation for the bacterial cell Factors inherent to the protein sequence responsible for aggregation and evolutionary mechanisms to keep proteins soluble Structural properties of proteins expressed as soluble aggregates and as inclusion bodies within bacterial cells both from a methodological point of view and with regard to their similarity with amyloids Control of the structural and functional properties of aggregated proteins and use thereof in biotechnology and medicine ''Protein Aggregation in Bacteria'' is ideal for researchers in protein science, biochemistry, bioengineering, biophysics, microbiology, medicine, and biotechnology, particularly if they are related with the production of recombinant proteins and pharmaceutical science "This question-and-answer guide on metalloproteins reflects the broad range of aspects within this branch of protein science. It is divided into seven parts, addressing: the cell, the alkali and alkaline earth cations, non-redox metalloenzymes, carboxypeptases, carbonic anhydrase, alcohol dehydrogenase, copper proteins, electronic and ESR spectra of copper ions, plastocyanin, stellacyanin, superoxide dismutase, electronic spectra of iron ions, Mossbauer spectroscopy of iron ions, ESR spectra of Iion (III), iron bioavailability, siderophores, non-heme iron proteins, ferritin, transferrin, dioxygenase-iron proteins, rubredoxin, 2Fe-2S Ferredoxins, 4FE-4S Ferredoxins and hiPIP, aconitase, hydroxylases, hydrogenases, nitrogenases, binuclear Fe proteins, hemerythrin, ribotide reductase, purple acid phosphate and methane mono-oxygenase, heme proteins, myoglobin and hemoglobin, electronic spectra of hemoproteins, cytochrome C, peroxidase, cytochrome P450, ESR spectra of hemoproteins, vitamin B12, and chlorophyll. Each section ends with suggestions for further reading"--Provided by publisher This book focuses on the aggregation of recombinant proteins in bacterial cells in the form of inclusion bodies. Recent reports revolutionized the current view of inclusion bodies from that of inert deposits of inactive proteins to reservoirs of proteins that can eventually maintain biological activity and/or be rescued by cells. Aggregation is put in the context of updated knowledge about the folding and aggregation of proteins in simple cells and new perspectives derived from the application of this knowledge are presented. The following topics are addressed: a) molecular and c