Progress in Plant Protoplast Research : Proceedings of the 7th International Protoplast Symposium, Wageningen, the Netherlands, December 6–11, 1987

**`**The amount of information included in the volume enables the reader to rapidly obtain a general overview of this field of research. In this respect it is of value to scientists already established in the field of plant genetic manipulation and those wishing to enter this exciting area of plant research.**'** **M.R. Davey, Plant, Cell and Environment,** Vol. 12, No. 5, 1989. **`**By the wide volume of data presented as well as topicality of the problems and the high scientific content, the book is a valuable guide and source of information in a modern and important field of biological sciences, for phytologists, cytologists, geneticians and pecialists in biotechnology, in general.**'** **A. Brezeanu, Romanian Review of Biochemistry,** 1989 Front Matter....Pages I-XVII Progress in Plant Protoplast Research....Pages 1-5 Obstacles and Perspectives in Protoplast Research....Pages 7-14 Aspects of Protoplast Culture and Plant Regeneration....Pages 15-25 Recent Developments in the Regeneration of Agronomically Important Crops from Protoplasts....Pages 27-31 Selection, Experimental Manipulation and Culture of Single Protoplasts....Pages 33-34 Uniparental, Fusant, and Chimeric Plants Regenerated from Protoplasts after Streak Plating in Agarose Gels....Pages 35-37 Electroporation-Mediated Enhancement of Plant Protoplast Division and Plant Regeneration....Pages 39-40 Selection of Potato Cell Suspension Protoplast Subpopulation by Rhodamine 123....Pages 41-42 Protoplast Culture Of Dioscorea Opposita Thunb. (D. Batatas DECNE)....Pages 43-44 Isolation and Culture of Buckwheat (Fagopyrum Esculentum Moench.) Callus Protoplasts....Pages 45-46 Partial Purification of a Cellulase Used for Protoplast Isolation from Barley ( Hordeum vulgare )....Pages 47-48 Plant Regeneration from Cell Suspension Protoplasts of Festuca Arundinacea Schreb., Lolium Perenne L. and L. Multiflorum Lam....Pages 49-52 Isolation, Culture and Regeneration of Lolium Perenne and Lolium Multiflorum Protoplasts....Pages 53-54 Mature Plant Regeneration from Cultivated Protoplasts of Rice ( Oryza Sativa L.)....Pages 55-56 The Plants Regenerated from Protoplasts of Suspension Cells of Rice(Oryza Sativa L)....Pages 57-58 Regeneration of Plantlets from Protoplasts of Poa Pratensis L. (Kentucky Bluegrass)....Pages 59-60 Somatic Embryogenesis and Plant Regeneration from Protoplast of Maize ( Zea mays L.)....Pages 61-62 Protoplasts from Actinidia Deliciosa (Kiwi): Obtention and Culture....Pages 63-64 Colony Formation from Cell Suspension Culture Protoplasts of Kiwifruit....Pages 65-66 Isolation and Culture of Grapevine Protoplasts (Vitis Vinifera L.)....Pages 67-68 Studies Towards a Protoplast Isolation and Culture Procedure in Sugarbeet ( Beta Vulgaris L.) Applicable to a Variety of Genotypes....Pages 69-70 Improvement of Protoplast Culture in Helianthus annuus (L)....Pages 71-72 Factors Influencing Protoplasts Plating Efficiency and Direct Somatic Embryogenesis Induction in Sunflower ( Helianthus Annuus L.)....Pages 73-74 Comparison of Sunflower Protoplast Development in Liquid or Solidified Media....Pages 75-76 Plant Regeneration from Protoplasts of Rudbeckia and Dimorphotheca Species ( Compositae )....Pages 77-78 Optimization of lettuce calli regeneration from protoplasts....Pages 79-80 Optimal Density and Osmolality of Protoplasts for Improved Cultural Performance....Pages 81-82 Development of Protoplast Culture Procedures in Brassica Crops Suitable for in Vitro Selection Herbicide Resistance Mutants and Cybrids....Pages 83-84 Somatic Embryogenesis in Stationary Phase Suspension Cultures Derived from Hypocotyl Protoplasts of Brassica Napus L.....Pages 85-90 Plant Regeneration from Cucumber Protoplasts....Pages 91-94 Callus Formation from Mesophyll Protoplasts of Forage Legume Astragalus Huangheensis Fu Et Lin....Pages 95-96 Preliminary Studies of Lupinus Protoplasts....Pages 97-98 Regeneration and Differentiation of Protoplasts in Grain Legumes....Pages 99-100 Plant Regeneration from Strawberry (Fragaria Ananassa) Mesophyll Protoplasts....Pages 101-102 Isolation and Culture of Apple Mesophyll Protoplasts....Pages 103-104 Rhizogenesis as an Intermediate Step towards Plant Regeneration of Protoplasts of Sour Cherry (Prunus Cerasus L.), Rosaceae....Pages 105-106 Isolation and Culture of Protoplasts from Totipotent Cell Cultures of Populus Ciliata ....Pages 107-108 A Protoplast System for Populus Which will Support Genetic Engineering Research of Tree Species....Pages 109-110 Factors Influencing Digitalis Obscura Mesophyll Protoplast Development....Pages 111-112 Cotyledon Protoplasts from Solanum Melongena L . Regeneration to Fertile Plants....Pages 113-114 Isolation, Culture and Regeneration of Leaf Protoplasts in the Genus Capsicum....Pages 115-116 Isolation and Properties of Protoplasts of Theobroma Cacao L.....Pages 117-118 Plantlet Regeneration from Protoplasts of Levisticum Officinale Koch.and Its Cytological Investigation....Pages 119-120 Differences in developmental morphology of protoplasts from two cell lines of white spruce ( Picea glauca )....Pages 121-122 Protoplast Development from Porphyra Linearis — An Edible Marine Red Alga....Pages 123-124 Structure and Physiology of the Protoplast Plasma Membrane....Pages 125-131 Why do Mesophyll Protoplasts Dedifferentiate?....Pages 133-134 Release of Phytotoxic Factors from Plant Cell Walls during Protoplast Isolation....Pages 135-136 Role of Peroxidase in Protoplast Development: The Activity and Molecular Forms of Peroxidase in Mitogenic and Non-Mitogenic Protoplasts....Pages 137-138 Measurement of Cytoplasmic Ca 2+ and H + in Barley Aleurone Protoplasts....Pages 139-142 The Effect of Secretion on Lipids and their Distribution in Barley Aleurone Protoplasts....Pages 143-144 Incidence of Growth Regulators on RNA/ DNA Levels in Protoplasts as Analysed by Acridine Orange Staining and Flow Cytometry....Pages 145-146 IAA, ABA Content and Metabolism in Maize Root Protoplasts....Pages 147-148 Some Metabolic Peculiarities of Isolated Protoplasts....Pages 149-150 Gene Transfer by Individual Manipulation of Plant Cells....Pages 151-157 Potentials of Protoplast Fusion in Plant Breeding Programmes....Pages 159-168 Use of Protoplasts on Plant Breeding: Cytoplasmic Aspects....Pages 169-176 Protoplast Fusion between Eruca Sativa and Brassica Napus — Analysis of Regenerated Plants....Pages 177-178 Fertility and Chromosome Stability in Brassica Napus Resynt-Hesised by Protoplast Fusion....Pages 179-180 Involvement of Protoplast Fusion in Cauliflower Breeding....Pages 181-182 Protoplast Fusion as a Method of Increasing Cytoplasmic Heterogeneity in Brassica Napus ....Pages 183-184 Selection of a Universal Hybridizer in Sinapis Turgida Del. and Regeneration of Plantlets from Somatic Hybrids with Brassica Species....Pages 185-186 Towards the Introduction of Cytoplasmic Male Sterility (CMS) into Brassica Napus Through Protoplast Fusion....Pages 187-188 Production of Somatic Hybrid Plants, ‘Brassicomoricandia’, Through Protoplast Fusion between Moricandia Arvensis and Brassica Oleracea ....Pages 189-190 The Use of Haploid Protoplast Fusion to Combine Cytoplasmic Atrazine Resistance and Cytoplasmic Male Sterility in Brassica Napus ....Pages 191-194 Somatic Hybridization of Diploid Potato Lines, a Tool in Potato Breeding....Pages 195-196 Somatic Hybridization between Anther-Derived Dihaploid Lines of Solanum Tuberosum L. (Potato)....Pages 197-198 Fusion of Dihaploid Solanum Tuberosum Protoplasts and Trials of Hybrid Selection with Phytotoxins....Pages 199-200 Production and Field Assessment of Somatic Hybrids between Dihaploid Solanum Tuberosum and Solanum Brevidens ....Pages 201-201 Fusion of Solanum tuberosum L. cv. Explorer(Potato) and Solanum melongena L. cv. Imperial Black Beauty(Eggplant) Protoplasts and Subsequent Callus Regeneration....Pages 203-204 “Somatic Fusions of Solanum Species”....Pages 205-207 Flow Cytometric Sorting of Potato Heterokaryons with Inhibition of Cell Wall Synthesis before Sorting....Pages 209-210 An Improved Peg Fusion Procedure for Flow Sorting of Plant Protoplasts....Pages 211-212 Regeneration and Evaluation of Somatic Hybrid Plants between Solanum Tuberosum and Lycopersicon Pimpinellifolium ....Pages 213-214 Studies on Gene Mapping in the Potato Using Somatic Hybrids of Solanum Tuberosum L. + Nicotiana Plumbaginifolia ....Pages 215-218 Interspecific Expression of AEC-Resistance in Somatic Hybrids of AEC-Resistant Solanum Tuberosum and Nitrate-Reductase-Deficient Nicotiana Plumbaginifolia....Pages 219-220 Interspecific Protoplast Fusion for the Transfer of Atrazine Resistance from Solanum Nigrum to Tomato ( Lycopersicon Esculentum .L)....Pages 221-224 Somatic hybridization between tomato and other Lycopersicon or Solanum species....Pages 225-226 Transfer of Regeneration Capacity from Lycopersicon Peruvianum to L. Esculentum by Protoplast Fusion....Pages 227-230 Somatic Hybridization in Lycopersicon; Using Gamma-Irradiated Nicotiana Tabacum SR-1 or Lycopersicon Peruvianum....Pages 231-232 Regeneration of Somatic Hybrid Plants between Tobacco ( Nicotiana Tabacum L.) and Black Nightshade ( Solanum Nigrum L.)....Pages 233-234 The Use of Streptomycin Resistance and Chlorophyll Deficiency for Selection of Somatic Hybrids between Nicotiana Tabacum and N. Rustica ....Pages 235-236 Intra- and Interspecific Gametosomatic Hybridisation within the Genus Petunia....Pages 237-240 The Transfer of Cytoplasmic Male Sterility (CMS) by Intraspecific Somatic Hybridisation in Petunia Hybrida (Hort) VILM....Pages 241-242 Application of Protoplast Fusion to an Ornamental Breeding Programme....Pages 243-244 Formation of Carrot Cybrids Based on Metabolic Complementation by Fusion between X-Irradiated Cells and Iodoacetamide-Treated Cells....Pages 245-248 Optimization of Conditions for Somatic Cybridization of Daucus Carota L....Pages 249-250 Isolation and Fusion of Apium Graveolens (Celery) and Levisticum Officinale (Lovage) Mesophyll Protoplasts....Pages 251-252 Electrofusion of Plant and Fungal Protoplasts....Pages 253-254 Regeneration and Analysis of Callus from Flow-Sorted Heterokaryons of Soybean ( Glycine Max ) and G. Canescens ....Pages 255-256 Interspecific Somatic Hybridization Studies in the Genus Lotus ....Pages 257-258 Interspectfic Hybrid Lines of M. Sativa and M. Arborea by Protoplast Electrofusion....Pages 259-260 Electrofusion and Electroporation of Sugar Beet ( Beta Vulgaris L.) Protoplasts....Pages 261-262 The Production and Electrofusion of Beta Cytoplasts....Pages 263-264 Inactivation of Sugarbeet Protoplasts Using Acridin Orange, an Agent for Late Selection of Fusion Products....Pages 265-266 Mitochondrial DNA Amplification in Somatic Hybrid Cells of Grasses....Pages 267-268 Plant Regeneration from Electrofused Protoplasts of Chrysanthemum Morifolium and Salpiglossis Sinuata ....Pages 269-270 Electrofusion of Chemically-Aggregated Protoplasts Derived from Mesophyll Tissue and Habituated Cells of Catharanthus Roseus....Pages 271-272 Studies on the Transfer of the Mitochondrial Genome Through Protoplast Fusion in Rice....Pages 273-274 Increase of Genetic Variability by Asymmetric Cell Hybridization and Isolated Chromosome Transfer....Pages 275-281 Cell-Mediated Gene Transfer....Pages 283-285 Protoplast Fusion of Kanamycin Resistant, CNX - Nicotiana Plumba-Ginifolia with Streptomycin Resistant N. Tabacum (SR1) and the Effect of Irradiation of the Tabacum Parent....Pages 287-288 Selecton for Resistance to Phoma Lingam Using Pycnospore Infections of Plants in-Vivo and Sirodesmin PL as Selective Agent in-Vitro ....Pages 289-292 Induction of Micronuclei by Amiprophos-Methyl and their Application for Transfer of Single, Specific Chromosomes in Plant Cells....Pages 293-296 Isolation of Microprotoplasts....Pages 297-298 Direct Cell to Cell Transfer of Mitochondria by Microinjection....Pages 299-300 Classification and Sorting of Plant Chromosomes Using Flow Cytometry....Pages 301-302 Application of Flow Cytometry for the Isolation of Metaphase Chromosomes from Protoplasts of Nicotiana Plumbaginifolia ....Pages 303-304 Electroporation for DNA Delivery to Plant Protoplasts....Pages 305-310 Electromanipulation of Protoplasts....Pages 311-314 Plant Protoplast Electropermeabilization: Role of Electrical and Biological Parameters....Pages 315-316 The Fusogenic State of Plant Protoplasts as Related to DYE/DNA Uptake....Pages 317-318 Genetic Manipulation of Plant Cells and Organelles with a Laser Microbeam....Pages 319-322 Electric Field Mediated DNA Uptake and Gene Expression in Mesophyll, Suspension Culture, and Tuber Protoplasts of Wild and Cultivated Potato....Pages 323-324 Introduction of Selectable Markers into Different Diploid Potato ( Solanum Tuberosum L .) Genotypes by Direct Gene Transfer....Pages 325-327 Direct Gene Transfer in Petunia Hybrida Protoplasts: Evidence for Co-Transformation....Pages 329-330 Electroporation of Tobacco Protoplasts: A Model System to Study Plant Gene Expression....Pages 331-332 Infection of Tobacco Protoplasts with RNA of a Pepper Strain of Tobacco Mosaic Virus by Electroporation....Pages 333-334 Comparison of Direct Gene Transfer into Nicotiana Protoplasts by Electroporation and Mg 2+ /PEG Methods....Pages 335-336 Quantification of Transient Expression Levels of Genes Transferred to Plant Protoplasts by Electroporation....Pages 337-338 Gene Transfer by Electroporation in Alnus Incana Protoplast....Pages 339-340 Transformation of Rice Protoplasts by Direct DNA Uptake....Pages 341-342 Isolation, Culture and Morphogenesis from Wheat Protoplasts, and Study of Expression of DNA Constructs by Direct Gene Transfer....Pages 343-346 UV Inducible Transient Expression of Chimeric CHS-NPTII in Parsley Proto — Plasts....Pages 347-348 Modification of Plants Using Intracellular Associations of L-Form Bacteria....Pages 349-350 Transformation of Brassica Nigra Through Protoplast Cocultivation with Agrobacterium Tumefaciens....Pages 351-352 Transformation of Brassica Species Via Direct Gene Transfer....Pages 353-354 Genetic Transformation of Diploid Solanum Tuberosum by Direct DNA Transfer and Mediated by Agrobacterium Tumefaciens....Pages 355-356 Optimization of Direct Gene Transfer in Tomato....Pages 357-358 Transformation Studies in Tobacco, Moth Bean and Brassica Species....Pages 359-362 Transposition and Regulation of the En Elements of Maize Assayed in Transformed Tobacco....Pages 363-364 Initiation of Cell Suspensions, Protoplast Culture, and Direct Gene Transfer to Protoplasts of Secale cereale L. (rye)....Pages 365-367 Protoplast Culture and DNA Uptake in Echinocloa....Pages 369-370 Transient Expression of Foreign Genes in Wheat Protoplasts....Pages 371-372 Protoplast culture and transformation studies of Triticale ( x Triticosecale Wittmack)....Pages 373-376 Analysis of the Zea Mays Sucrose Synthase Promotor by Transient Gene Expression in Maize Protoplasts....Pages 377-378 Protoplast Culture of 2n S. Tuberosum as a Source of Mutant Phenotypes....Pages 379-380 Cytoplasmic or Nuclear Control of Male Sterility in Protoplast-Derived Plants of Nicotiana Sylvestris ....Pages 381-382 Selection and Characterization of Nitrate Reductase-Deficient Mutants of Nicotiana Plumbaginifolia ....Pages 383-385 Isolation of Temperature-Sensitive Auxin-Requiring Variants of Nicotiana Plumbaginifolia....Pages 387-388 The use of phosphinothricin resistance as a selectable marker in tobacco protoplast transformation....Pages 389-390 Selection for Salt/Drought Tolerance Using Isolated Protoplasts and Protoplast-Derived Calli of Colt Cherry (Prunus Avium X Pseudocerasus)....Pages 391-392 Field Tests of Progeny from Protoplast Regenerated Oilseed Rape....Pages 393-394 Studies of lettuce plants regenerated from protoplasts....Pages 395-396 Somaclonal Variations for Indole Alkaloid Accumulation in Protoclones Derived from an Habituated Strain of Catharanthus Roseus....Pages 397-398 Somaclonal Variations of Dihydrofuroquinoline Alkaloids in Cell Lines Issued from Strains and Leaf Mesophyll of Choisya Ternata by Protoplast Cloning....Pages 399-400 Some Aspects of Salinity Tolerance of Calli Derived from Leaf Tomato Protoplasts....Pages 401-402 Proto-Variation in Protoplast Derived Brassica Napus Plants....Pages 403-404 Back Matter....Pages 405-412 ` The amount of information included in the volume enables the reader to rapidly obtain a general overview of this field of research. In this respect it is of value to scientists already established in the field of plant genetic manipulation and those wishing to enter this exciting area of plant research. ' M.R. Davey, Plant, Cell and Environment, Vol. 12, No. 5, 1989. ` By the wide volume of data presented as well as topicality of the problems and the high scientific content, the book is a valuable guide and source of information in a modern and important field of biological sciences, for phytologists, cytologists, geneticians and pecialists in biotechnology, in general. ' A. Brezeanu, Romanian Review of Biochemistry, 1989