Progress in Photosynthesis Research : Volume 2 Proceedings of the VIIth International Congress on Photosynthesis Providence, Rhode Island, USA, August 10-15, 1986

These Proceedings comprise the majority of the scientific contributions that were presented at the VIIth International Congress on Photosynthesis. The Congress was held August 10-15 1986 in Providence, Rhode Island, USA on the campus of Brown University, and was the first in the series to be held on the North American continent. Despite the greater average travel distances involved the Congress was attended by over 1000 active participants of whom 25% were registered students. This was gratifying and indicated that photosynthesis will be well served by excellent young scientists in the future. As was the case for the VIth International Congress held in Brussels, articles for these Proceedings were delivered camera ready to expedite rapid publication. In editing the volumes it was interesting to reflect on the impact that the recent advances in structure and molecular biology had in this Congress. It is clear that cognizance of structure and molecular genetics will be even more necessary in the design of experiments and the direction of future research. Front Matter....Pages I-XXVII Structural Principles of the Antenna System of Photosynthetic Organisms....Pages 1-8 The Molecular Size of Photosynthetic Membrane Protein Complexes....Pages 9-11 Structural Studies on the Light-Harvesting Polypeptides of RP. Acidophila....Pages 13-16 Localization and Comparative Chemical Activity of SH-Groups of the Subunits in the Reaction Centers from Rhodopseudomonas Sphaeroides R-26....Pages 17-19 Topological Studies on the Light-Harvesting-Pigment-Protein-Complexes in the Membrane of Rhodospirillum Rubrum ....Pages 21-24 Preparation of Subunit Forms of the Light-Harvesting Complex of Rhodospirillum Rubrum ....Pages 25-28 Chemical Modification of Tryptophans and Lysines in The B880 Light-Harvesting Protein of Rhodospirillum Rubrum ....Pages 29-32 Studies of Chloroplast Photosystem I Using Monoclonal Antibodies....Pages 33-36 Bound Acceptors of Photosystem I....Pages 37-40 Photosystem I Complex of Higher Plants: Purification and Characterization of its Two Chlorophyll-Protein Complexes....Pages 41-44 Preparation and Properties of Photosystem-1 Reaction Center Complex....Pages 45-48 The Organization of the Fe-S Acceptors of Photosystem 1....Pages 49-52 Analysis of Isolated PS I Polypeptides for Acid Labile Sulfide....Pages 53-56 Effects of Mercurials on Iron-Sulfur Centers of Photosystem I of Anacystis Nidulans ....Pages 57-60 Light-Harvesting Chlorophyll-Proteins of Barley Photosystem I....Pages 61-64 Measurement of the Optical Cross Section of Photosystem I in Chlorella....Pages 65-68 Isolation of a Photosystem II Reaction Center Consisting of γ and δ Subunits (D-1 and D-2) and Cytochrome b-559....Pages 69-72 Quantitation of Plastoquinone and Functional Electron Carriers in the Photosystem II Reaction Center Complex....Pages 73-76 Identification of Q A -, Q B - and Manganese-Carrying Subunit Polypeptides of Synechococcus Photosystem II Reaction Center Complex....Pages 77-80 The Organisation of Photosystem II Chlorophyll-Proteins....Pages 81-88 Phosphorylated Polypeptides of the Photosystem 2 Core Complex....Pages 89-92 Stoichiometry and Turnover Rates of Photosystem 2 Core Polypeptides of the Halotolerant Green Alga Donaliella Salina ....Pages 93-96 Variable and Conserved Characteristics of Photosystem 2 of Spinach and of the Halotolerant Green Alga Dunalliela Salina ....Pages 97-100 Organization of Photosystem II Core and Light-Harvesting Complexes....Pages 101-104 Chemical Composition of Photosystem II Reaction Centers (PSII); Phosphorylation of PSII Polypeptides....Pages 105-108 Topology of Polypeptides and Redox Components in PS II and the Cytochrome b/f-Complex....Pages 109-112 Does the Rapidly Metabolized 32 kDa Protein Carry the Reaction Center of PS II?....Pages 113-116 Analysis of Photosystem II Proteins by Two-Dimensional Electrophoresis....Pages 117-120 The 5 kDa, 10 kDa and 22 kDa Polypeptides of Higher Plant Photosystem II....Pages 121-124 Low Molecular Weight Polypeptides in Photosystem II and Protein Dependent Acceptor Requirement for Photosystem II....Pages 125-128 Characterization of a Monoclonal Antibody which Reacts with the 49 kDa Polypeptide of Photosystem II from Spinach....Pages 129-132 Oxidation Reduction Properties of the Iron (Q 400 ) in PSII in the Cyanobacterium Synechocystis 6714 ....Pages 133-136 Pigment Composition of Pigment-Protein Complexes from the Dinoflagellate Gonyaulax Polyedra ....Pages 137-140 Monoclonal Antibodies to the Light Harvesting Chlorophyll-Protein Complex....Pages 141-144 Isolation and Immunological Characterization of a Carotenoid-Binding Protein from the Cyanobacteria Synechocystis Sp. PCC6714 and Anacystis Nidulans R2....Pages 145-148 Isolation and Characterization of A Carotenoid-Associated Membrane Protein from The Cyanobacterium, Anacystis Nidulans R2....Pages 149-152 Structural Principles of Cryptomonad Biliproteins and Cyanobacterial Phycobiliproteins....Pages 153-156 Non-Hemidiscoidal Phycobilisome in Cyanophytes....Pages 157-160 Comparative Immunology of the Phycobilisome Linker Polypeptides....Pages 161-164 Structural Studies on Cytochrome b6 and the Rieske FeS-Center....Pages 165-172 Large-Scale Isolation of Cytochrome b 6 / f Complex from Spinach Chloroplasts....Pages 173-176 Isolation and Characterization of the Qbc -Complex from the Thermophilic Cyanobacterium Synechococcus 6716....Pages 177-180 Photosynthetic Pigment Organization in Membranes of Eucartotic Plants....Pages 181-184 Organization of the Photosynthetic Apparatus in Macrocystis Pyrifera ....Pages 185-188 Pigment-Protein Complexes and Light Harvesting in Eustigmatophyte Algae....Pages 189-192 Immunological Studies on the Chlorophyll a + b Antenna Complexes of Photosystem I and Photosystem II....Pages 193-196 Large-Scale Preparation of Highly Purified Chloroplast Lipids for Liposome Permeability Studies....Pages 197-200 The Association between Galactolipids and Chlorophyll-Protein Complexes....Pages 201-204 Galactosyltransferase Activities in Intact Spinach Chloroplasts and Envelope Membrane Involvement of Galactolipid : Galactolipid Galactosyltransferase ....Pages 205-208 Characterization of Galactosyltransferases in Spinach Chloroplast Envelope Membranes. UDPgal-Dependent and -Independent Galactolipid Synthesis....Pages 209-212 Effects of Monogalactosyldiacylglycerol (MGDG) Depletion on Photosynthetic Activities in OAT Thylakoid Membranes....Pages 213-216 Galactolipids and Thylakoid Intramembrane Particles....Pages 217-220 Reconstitution of Functional Phycobilisome-Thylakoid Association in Cyanobacteria....Pages 221-224 Molecular Organization of the Inner Etioplast Membranes....Pages 225-228 Surface Properties of the Prolamellar Body Membrane....Pages 229-232 P700 Spectra and Concentrations in Several Plants and a Cyanobacterium....Pages 233-236 Recovery Time of Physiologically Inactive Photosystem II Reaction Centers Measured by the Electrochromic Absorbance Change at 515nm....Pages 237-240 Identification of Photosystem II Centers Inactive in Plastoquinone Reduction....Pages 241-244 Photosystem Stoichiometry in Higher Plant Chloroplasts....Pages 245-248 Cause and Effect Relationship Between Environmental Conditions and Photosystem Stoichiometry in Synechococcus 6301....Pages 249-252 Differential Phosphorylation of Thylakoid Membrane Polypeptides During Chloroplast Adaptation to Light Quality....Pages 253-256 The Physiological Significance of Thylakoid Membrane Protein Phosphorylation....Pages 257-260 Development of The Light-Harvesting Antenna of the Photosystems in Chlorophyll- b Deficient Mutants....Pages 261-264 Comparative Studies on the Light Absorption Capacity of Photosystem II and Photosystem I....Pages 265-268 An Analysis of the Salt-Induced Rise of Chlorophyll Fluorescence to Investigate Ion-Specific Effects Between and within Valency Groups....Pages 269-272 Differential Thylakoid Membrane Stacking in Mangrove and Spinach....Pages 273-276 The Role of LHCII in Thylakoid Membranes....Pages 277-280 Subfractionation of Inside-Out Thylakoid Vesicles-Preparation of Pure Photosystem II Particles without using Detergent....Pages 281-284 Composition of the Marginal Regions of Thylakoid Membranes....Pages 285-288 Effect of Divalent Cations on the Aging of Wheat Chloroplasts....Pages 289-292 Cytochrome F Exists as Two Functionally Separate Pools in Pea Thylakoid Membranes....Pages 293-296 Structural Identification of the Oxygen-Evolving Apparatus on the Lumenal Surface of Freeze-Etched Thylakoid Membranes....Pages 297-300 Lateral Distribution of the Photosystem I Complex between the Appressed and Non-Appressed Regions of Spinach Thylakoid Membranes: An Immunocytochemical Study....Pages 301-304 Differential Solubilization of the Integral Electron Transport Complexes from the Thylakoid Membrane of Spinach Chloroplasts. Localization of Photosystem I, Photosystem II and the Cytochrome B 6 -F Complex....Pages 305-308 Immunogold Localization of the Phycobiliprotein of a Cryptophyte Alga to the Intrathylakoidal Space....Pages 309-312 Immunogold Labeling of Thylakoid Membranes....Pages 313-315 Assignments of Stroma-Exposed PSII Proteins after Two-Dimensional Electrophoresis using Affinity Purified Polyclonal Antibodies....Pages 317-320 Determination of the Insideout to Rightsideout Ratio of: 1) Thylakoids Exposed to Phase Partition, 2) Thylakoids Washed with Edta, and 3) Detergent Derived PSII Membranes....Pages 321-324 Localization of the b 6 /f Complex By Freeze-Fracture Analysis of the Chlamy-Pomonas Reinhardtii and Chlorella Sorokiniana Mutants Lacking in this Complex....Pages 325-328 Immunocytochemical Studies on the Organization of Thylakoid Membrane Proteins....Pages 329-332 Collapse Mechanism of some Carotenoid Monomolecular Films - Membrane Model....Pages 333-337 Effect of Daylength on Thylakoid Composition and Ultrastructure in Brassica Rapa Ssp . Oleifera ....Pages 339-342 Adaptation of Barley Thylakoid Membranes to Changing Light Intensity....Pages 343-346 Pigment-Protein Complexes of Algal Thylakoid Membranes: Variations in Pattern, Pigment Composition and Reaction Centre II Types During The Cell Cycle of Chlorella Fusca and after Adaptation to Low Light Intensities....Pages 347-350 On the Protective Role of Carotenoids in the Ps I Reaction Centre and LHCI Complexes of The Thylakoid Membrane....Pages 351-354 Response of Chlamydomonas Reinhardtii Reaction Center Stoichiometry to Light Intensity Variation: A Spectroscopic and Lds-Page Analysis....Pages 355-358 Effects of Nitrate Nutrition on Chloroplast Composition and Photosynthetic Activities in Wheat....Pages 359-362 The Effect of Different Growth Light Intensities On Photosystem II Components....Pages 363-366 Changes in Pool Sizes of Photosynthetic Electron Transport Components and Rubisco with Growth Irradiance - Evidence for the Limiting Step in Light Saturated Photosynthesis....Pages 367-370 Chlorophyll-Protein Complexes Changes Associated with Chloroplast Development in a Virescent Petunia Hybrida Mutant....Pages 371-374 Altered Photosynthetic Pigment Complements in Mutants of the Cyanobacterium Synechococcus Sp . Spp 7002....Pages 375-377 Characterization of a Novel Chlorophyll-Binding Component of Cyanobacterial Thylakoids....Pages 379-382 Some Aspects of Altered Structure and Functioning of the Photosynthetic Apparatus in Phytochrome-Less Mutants of Tomato....Pages 383-386 Some Functional Characteristics of Reaction Centers from Rhodopseudomonas Viridis ....Pages 387-396 Herbicide-Resistant Reaction Center Mutants of Rhodopseudomonas Viridis ....Pages 397-400 Light Induced Proton Binding-Unbinding Dynamics in Reaction Centers from Rhodobacter Sphaeroides ....Pages 401-404 An Inhibitor-Resistant Mutant Impaired in Ubiquinol Oxidation in the Ubiquinol Cytochrome C 2 Oxidoreductase of Rhodobacter Capsulatus ....Pages 405-408 Stigmatellin and Other Electron Transfer Inhibitors as Probes for the Q B Binding Site in the Reaction Center of Photosynthetic Bacteria....Pages 409-412 Q Pool Interactions in the Reaction Center-Cytochrome Bc 1 Complex Hybrid Proteoliposome System ....Pages 413-416 Reconstitution of Subunits LM of the Reaction Center from Rhodopseudomonas Sphaeroides into Liposomes....Pages 417-419 Covalent Binding of Halogen Substituted 1,4-Quinones to Proteins of the Thylakoid Membrane....Pages 421-424 Competition of Inhibitors with The Secondary Quinone in Dark Adapted Thylakoid Membranes....Pages 425-428 Kinetics of the Changes in Oxidation-Reduction States of the Acceptors and Donors of Photosystem II in Pea Thylakiods Measured by Flash Fluorescence....Pages 429-432 The Effect of pH and Flash Frequency on Electron Transfer Through the Quinone Acceptor Complex of Ps II in Bicarbonate Depleted or Anion Inhibited Thylakoid Membranes....Pages 433-436 Manipulations of the Pool Characteristics of the Quinone Electron Donor and Acceptor to Ubiquinol-Cytochrome c Oxidoreductase. Evidence for Redox Contact between the Cytochrome b Hemes....Pages 437-440 Inhibition of Electron Transfer in the Cytochrome b / f Complex By NQNO and DBMIB: Evidence for Two Quinone Binding Sites....Pages 441-444 The Electrogenic Reaction and Proton Release During Quinol Oxidation by the Cytochrome b / F Complex....Pages 445-452 Electron and Proton Transfer in The Cytochrome bf Complex....Pages 453-460 The Effect of Pre-Reduction of the b -type Cytochromes on Electron Transport Through the Cytochrome bf Complex in Chloroplasts....Pages 461-464 Studies of the Chloroplast Cytochrome b 6 -f Complex Incorporated into Liposomes....Pages 465-468 The Plastoquinone/Plastoquinol Oxidoreductase Sites of the Chloroplast Cytochrome b/f Complex....Pages 469-472 Proposed Alignment of Amino Acid Sequences for Cytochromes c1 and f and Identification of Putative Binding Sites for Cytochrome c and Plastocyanin....Pages 473-476 Quinol Specificity of the Cytochrome b6f-Complex - Evidence against a Permanently Bound Plastoquinone Molecule....Pages 477-480 On the Trans-Membrane Electron Transport Pathway of Cytochrome b 6 ....Pages 481-484 The Antimycin-binding Site of Thylakoid Membranes from Chloroplasts....Pages 485-488 Inhibition of High Energy State Quenching in Spinach Chloroplasts by Low Concentrations of Antimycin A....Pages 489-492 Electron Transfer from Quinol to Cytochrome B-561 is not Diffusion Limited in the Ubiquinol: Cyt C 2 Oxid Oreductase Complex of Rps. Sphaeroides ....Pages 493-496 Characterization by EPR Spectroscopy of Cytochrome b -562 Isolated from the Cytochrome b - c 1 Complex of Rhodopseudomonas Sphaeroides R-26....Pages 497-500 Plastoquinol and Plastoquinone Diffusion in Model Membranes....Pages 501-504 Temperature Dependence of DF-Kinetics and PQ-conductivity....Pages 505-508 A Carbon-13 NMR Study of Plastoquinone in Membranes....Pages 509-512 Lateral Electron Transport in Thylakoids of Higher Plants....Pages 513-520 On the Electron Transfer from Plastocyanin to P700....Pages 521-524 Use of Affinity Chromatography to Purify Plastocyanin and Cytochrome f....Pages 525-528 The Effect of Ethylenediamine Chemical Modification on the Electron Transport Properties and Redox Potential of Spinach Plastocyanin....Pages 529-532 The Effect of pH on the near UV Circular Dichroic Spectra of Parsley Plastocyanin....Pages 533-536 Ferredoxin-Dependent Cyclic Electron Transport and Photophosphorylation....Pages 537-540 Cyclic Electron Transport Around Photosystem I in Washed Thylakoids....Pages 541-544 Ferredoxin-NADP + Oxidoreductase: Studies on the Thylakoid Membrane Bound Enzyme....Pages 545-548 Inhibitors of Ferredoxin-Catalysed Cyclic Photphosphorylation in Chloroplasts....Pages 549-552 Photosynthetic Control of Electron Transport in Leaves of Phaseolus Vulgaris : Evidence for Regulation of Photosystem 2 by the Proton Gradient....Pages 553-556 Modification of the Redox State of PSII Electron Acceptors In Vivo by Low Levels of Intercellular Co 2 ....Pages 557-560 Analysis of the α and β Centres of Photosystem II, in Presence of Atrazine and TMPD....Pages 561-564 Senescence of Chloroplasts in Fern Leaves....Pages 565-568 The Role of Cu in the Regulation of the Photosynthetic Electron Flow....Pages 569-572 Copper in Photosystem II....Pages 573-576 Inhibition of the Photosynthetic Electron Transport of Isolated Chloroplasts by Hemolyzed Rabbit Sera....Pages 577-580 Effect of Dimethylaminoethyl p-Chlorophenoxyacetate (Centro-Phenoxine) on Chloroplast Electron Transport Activity....Pages 581-584 Effect of Resorcinols on Electron Transport in Pea Chloroplasts....Pages 585-587 Ascorbate As A Pro-Oxidant and an Anti-Oxidant During a Mehler Reaction....Pages 589-592 Effects of Deuterium Oxide on Photosynthetic Light Reactions....Pages 593-596 Senescence and Heat Stress Induced Alterations of Photosynthetic Electron Transport....Pages 597-604 Oscillation in Photosystem II Activity of Chloroplasts Isolated from Dark Adapted Wheat Seedlings....Pages 605-608 Diurnal Changes in Electron Transport Capacity in Pea Thylakoids....Pages 609-612 Modelling of Photosynthetic Energy Conversion I Description and Basic Principles of the Model....Pages 613-616 Modelling of Photosynthetic Energy Conversion. II. Charge Separation and Secondary Electron Flow....Pages 617-620 Modelling of Photosynthetic Energy Conversion III. Electrochemical Events at the Membrane....Pages 621-624 Photoacoustic Action Spectra of Bundle-Sheath Cells of Zea Mays....Pages 625-628 Photoacoustic Study of Photosynthetic Activity in Bundle-Sheath Cells of Zea Mays....Pages 629-632 Sulfide-Dependent Electron Transport in Thylakoids from the Cyanobacterium Oscillatoria Limnetica....Pages 633-636 Energetics of Hydrogen Recycling in Nitrogen-Fixing Cyanobacteria....Pages 637-640 Photosynthesis, Nitrogen Fixation, Ammonia Photoproduction and Structure of Anabaena Azollae Immobilized in Natural and Artificial Systems....Pages 641-644 Sustained Ammonia Production by Immobilized Cyanobacteria....Pages 645-648 Cellular Biochemical Activities and Induction of Synchronous Growth in a Unicellular Aerobic Nitrogen-Fixing Cyanobacterium, Synechococcus Sp. Strain Miami BG 043511....Pages 649-652 Inhibition of Respiration By Short Saturating Flashes in Chlamydomonas : Evidence for a Chlororespiration....Pages 653-656 Studies on the Linking of Photosynthetic and Respiratory Ellectron Transport Chains and Phosphorylation....Pages 657-660 Cytochrome Oxidase of Photosynthetically Grown Rhodopseudomonas Sphaeroides ....Pages 661-664 A Portable Double-Flash Spectrophotometer for Measuring the Kinetics of Electron Transport Components in Intact Leaves....Pages 665-668 Dynamic Aspects on the Organization of the Thylakoid Membrane....Pages 669-676 Different Polypeptide Composition of the Peripheral and Tightly Bound Subpopulations of LHCII....Pages 677-680 Interplay between Environmental and Metabolic Factors in the Regulation of Electron Transport in Higher Plants....Pages 681-688 The Importance of Membrane Surface Electrical Charge on the Regulation of Photosynthetic Electron Transport by Reversible Protein Phosphorylation....Pages 689-696 A Light-Sensitive Photoregulatory Mutant in Maize Deficient in LHCI and the ‘Mobile’ Chlorophyll a/b LHCII....Pages 697-700 Immunolocalization of the CHL a/b-Light Harvesting Complex and CP29 under Conditions Favoring Phosphorylation and Dephosphorylation of Thylakoid Membranes (State 1- State 2 Transitions)....Pages 701-704 Comparison between The Effects of Cations, State I-State II Transitions and Protein-Phosphorylation on Lifetime Components of Chlorophyll Fluorescence....Pages 705-708 Does a Correlation between the Xanthophyll Cycle and State 1 -State 2 Changes Exist?....Pages 709-712 Altered Lipid Matrix Changes Energy Distribution in Thylakoids....Pages 713-716 Energy Pipeline Model of the Photosynthetic Apparatus....Pages 717-720 Influence of Thylakoid Protein Phosphorylation on PS I Dependent Photophosphorylation and on Electron Transport....Pages 721-724 The Kinetics of Phosphorylation of the Light Harvesting Chlorophyll a/b Protein and Associated Changes in Photosystem Cross Section and Energy Spillover....Pages 725-728 Reversal Movement of Sub-Population of Light Harvesting Chlorophyll Protein Complexes LHC-II and LHC-I between Grana and Stroma Lamellae under Different Conditions....Pages 729-732 A possible redistribution of excitation energy in spinach chloroplasts due to nitrite ions....Pages 733-736 Effect of Light State Transitions On the Apparent Absorption Cross Section of Photosystem II in Chlorella ....Pages 737-740 Multiple Effects of Trypsin- and Heat-Treatments on the Ultrastructure and Surface Charge Density of Pea Chloroplast Membranes. Influence on P700 + Parameters....Pages 741-744 Steady-State Kinetics of Chlorophyll-Fluorescence and Light Scattering....Pages 745-748 Extreme Changes in the Distribution of Excitation Energy in the Photochemical Apparatus of Intact Leaves Induced by Progressive Heat Stress....Pages 749-752 Environmental Control of Photosynthetic Enhancement in Palisade Mesophyll Chloroplasts Measured by Photoacoustic Spectroscopy....Pages 753-756 Regulation of Photosynthetic Unit Function by Protein Phosphorylation....Pages 757-760 The 18.5 kDa Phosphoprotein of the Cyanobacterium Synechococcus 6301 : A Component of the Phycobilisome....Pages 761-764 State 1-State 2 Transitions in Synechococcus 6301 are Influenced by Respiratory Electron Flow; Evidence for the Control of Excitation Energy Distribution by the Redox Level of Plastoquinone in Cyanobacteria....Pages 765-768 Control of State 1 - State 2 Transitions in the Blue-Green Alga Nostoc Muscorum....Pages 769-772 The Relationships between Protein Kinase Activity and Chlorophyll a Fluorescence Changes in Thylakoids from the Cyanobacterium Synechococcus 6301....Pages 773-776 Excitation Energy Transfer in Cryptomonas Ovata . Preillumination Dependent Changees in 77K Picosecond Time Resolved Fluorescence Emission Spectra....Pages 777-780 Excitation Energy Redistribution in Cryptomonas Ovata ....Pages 781-784 Functional Interactions of Anions and Cations with the outer Surface of Intact Thylakoids: Studies with the Blue-Green Bacterium Anacystis Nidulans National Research Center Demokritos, Department of Biology, Athens 153 10, Greece....Pages 785-788 Effect of Growth Temperature on Membrane Properties in a Thermophilic Cyanobacterium ( Synechococcus Sp.)....Pages 789-792 Increased Energy Transfer from the Phycobilisomes to Photosystem II in High Light Adapted Anabaena Cylindrica....Pages 793-796 Purification and Characterization of a Thylakoid Protein Kinase....Pages 797-800 Reconstitution of LHC Phosphorylation by a Protein Kinase Isolated from Spinach Thylakoids....Pages 801-804 Identification of Phosphorylated Reaction Center Polypeptides in Thylakoids of Chlamydomonas Reinhardtii and Pisum Sativum ....Pages 805-808 The Sensitivity of Pisum Sativum Thylakoid Membrane Protein Kinase Activity to Inhibitors, Detergents and Heating....Pages 809-812 Back Matter....Pages 813-826