Practical Liquid Chromatography

This book is intended to provide a practical introduction to high-speed, high-efficiency liquid chromatography. It covers modern column technology (which has leapt into prominence only in the last five years) and relates this to the well-established thin-layer techniques. The development of liquid chromatography has proceeded in fits and starts over many years and in alliance with various scientific disciplines. Liquid chromatography has for years fulfilled an effective role in various fields. Ion-exchange chromatography, for example, is particularly associated with the separation of the rare earths, and exclusion chromatography with the fractionation of naturally occurring materials like proteins and of syn­ thetic polymers. Partition chromatography, especially in the form of paper chromatography, has been an indispensable tool in the study of biochemical systems, while its more recent adsorption counterpart, thin-layer chromatog­ raphy, developed most rapidly within the pharmacognosic and pharma­ ceutical fields. Until recently, however, liquid chromatography has not played a prominent role in the field of industrial organic analysis. 1: Liquid Chromatography-The Background.- 1.1. Perspective.- 1.2. Plan of the Book.- 1.3. Chromatography and Its Basic Variants.- 1.3.1. Adsorption Chromatography.- 1.3.2. Ion-Exchange Chromatography.- 1.3.3. Partition Chromatography.- 1.3.4. Exclusion Chromatography.- 1.4. Development Methods in Chromatography.- 1.4.1. Elution.- 1.4.2. Frontal Analysis.- 1.4.3. Displacement.- 1.5. Basic Experimental Arrangements.- 1.5.1. Column Chromatography.- 1.5.2. Layer Chromatography.- 1.6. Measures of the Effectiveness of Chromatographic Separations.- 1.6.1. HETP.- 1.6.2. Resolution.- 1.7. Retention Par Front Matter....Pages i-xiii Liquid Chromatography—The Background....Pages 1-12 Band Spreading in Chromatography....Pages 13-39 Adsorption Chromatography: Mechanism and Materials....Pages 41-73 Liquid Partition Chromatography—Mechanism and Materials....Pages 75-92 Exclusion Chromatography—Mechanism and Materials....Pages 93-113 The Technique of Thin-Layer Chromatography....Pages 115-164 Equipment for Column Chromatography....Pages 165-209 Current Status of Liquid Chromatography: Place Among Family of Chromatographic Techniques....Pages 211-219 Back Matter....Pages 221-230