Plasma Membrane Oxidoreductases in Control of Animal and Plant Growth

The objective of this workshop was to examine the nature of plasma membrane electron transport and how this electron transport contributes to growth of cells. The workshop came at a time when the study of the plasma membrane oxidoreductase activity was beginning to attract more widespread attention from researchers working with both plants and animals. The rapid response of c fos and c myc Proto-oncogene to stimulation of plasma membrane redox activity by external oxidants under­ scores a potential role of plasma membrane oxidoreductases in growth control. Other experiments with isolated endosomes in­ dicate emerging roles in endocytosis and lytic processes. Primary attention was focused on trans plasma membrane electron transport which brings about the oxidation of cytosolic, NADH, NADPH or other substrates by electron flow across the plasma membrane to external oxidants including ferric iron, semide­ hydroascorbate or oxygen. A major theme in the workshop was the relation of this electron flow to pH changes of the cytoplasm or the transfer of protons to the external medium. The presence and role of other oxidoreductases in the plasma membrane was documented, especially in regard to peroxide production. In plant cells this may contribute to cellular defense against invading para­ sites. A corresponding function in animals has been long known and extensively discussed but was beyond the scope of this workshop. Front Matter....Pages i-xv Introductory Remarks....Pages 1-5 Modulation of Redox Reactions Involved in DNA Synthesis by Oxygen and Artificial Electron Acceptors....Pages 7-16 Redox Agents which Modulate the Growth of Melanoma Cells: Evaluation of Mechanisms....Pages 17-26 Possible Role of Transplasma Membrane Ferricyanide Reductase in Mitogenic Activation of Rat Liver Cells....Pages 27-36 The Requirement for Transmembranal Electron Flow for Cell Proliferation....Pages 37-44 Roles for Plasma Membrane Redox Systems in Cell Growth....Pages 45-55 Redox Components in the Plant Plasma Membrane....Pages 57-69 Reduction-Oxidation Activities of Plant Plasmamembranes....Pages 71-80 Purification of NADH-Ferricyanide and NADH-Duroquinone Reductases from Maize ( Zea mays L.) Root Plasma Membranes....Pages 81-88 The Turbo Reductase in Plant Plasma Membranes....Pages 89-93 Specific Interaction of the Herbicide Sethoxydim with the Plasmalemma Redox System of Plant Cells....Pages 95-104 NADH or NADPH ?....Pages 105-114 Mechanism and Control of Iron Uptake by Erythroid and Non-Erythroid Cells....Pages 115-116 The Release of Iron from a Subfraction of Rat Liver Highly Enriched in Endosomal Organelles Requires Both a Functional H + -ATPase and NADH....Pages 117-126 Uptake of Iron from Ferric Iron Compounds by Isolated Hepatocytes....Pages 127-133 Hepatocyte Iron Uptake - A Plasma Membrane Process or an Endosomal Process ?....Pages 135-137 Reductive Release of Iron From Transferrin and Receptor Mediated Recycling....Pages 139-151 Transplasma Membrane Electron Transport Functions as a Ferric Reductase....Pages 153-161 Modulation of Transferrin Receptor Gene Expression by Peptide Growth Factors and Role of the Receptor in Human Fibroblasts....Pages 163-171 Basic and Clinical Studies of Transferrin-Adriamycin Conjugates: Mechanisms for a New Approach to Drug Targeting....Pages 173-180 Control of Transplasma Membrane Diferric Transferrin Reductase by Antitumor Drugs....Pages 181-190 A General Introduction to the Link between Redox Reactions and Proton Gradients....Pages 191-197 Regulation of Proton Diffusion Pathways in Chloroplast Thylakoids by Ca ++ Gating....Pages 199-208 Energy-Dependent Transport of Urate and Xanthine in the Unicellular Green Alga Chlamydomonas Reinhardtii ....Pages 209-217 Mechanisms of Ion Transport in Plants: K + as an Example....Pages 219-232 Electron Transport at the Plasma Membrane and ATP-Driven H + Extrusion in Elodea densa Leaves....Pages 233-241 The Relationships Between Ferricyanide Reduction and Associated Proton Efflux in Mechanically Isolated Photosynthetically Competent Mesophyll Cells....Pages 243-252 The Ferricyanide-Driven Redox System at the Plasmalemma of Plant Cells: Origin of the Proton Production and Reappraisal of the Stoichiometry e − /H + ....Pages 253-262 Ferricyanide Changes the Transport Properties of Lamprothamnium Papulosum Plasmalemma....Pages 263-271 b-Type Cytochromes, Light- and NADH-Dependent Oxido-Reductase Activities in Plant Plasma Membranes....Pages 273-282 Kinetics of Redox State as Related to Plant Growth and Development....Pages 283-285 Regulation of Transplasmalemma Electron Transport by Calcium and Light in Oat Mesophyll Cells....Pages 287-293 Thioredoxin and Glutaredoxin: Functions in Growth-Dependent DNA Synthesis and Cellular Regulation Via Thiol Redox Control....Pages 295-302 Cytochrome b 561 and the Maintenance of Redox Poise in Secretory Vesicles....Pages 303-312 The Retinoic Acid-Induced Cascade of Events Leading to Granulocyte Differentiation in HL60 through a Postulated GTP-Regulated Inducing Molecule....Pages 313-321 Changes in Plasmalemma Functions Induced by Phytopathogenic Bacteria....Pages 323-332 On the Occurrence of Oxidoreductases in the Apoplast of Leguminosae and Gramineae and their Significance in the Study of Plasmamembrane-Bound Redox Activities....Pages 333-337 Relationship between NAD + /NADH Levels and Animal Cell Growth....Pages 339-347 Redox Control of Glutathione and Thioredoxin Reductases....Pages 349-358 Cardiac Ca 2+ Channels and Sarcolemma Redox....Pages 359-368 Role of Na + /H + -Antiporter in Growth Stimulation by Ha-ras....Pages 369-381 Protein Kinase C: Plasma Membrane to Nucleus....Pages 383-393 Plasma Membrane Cytochrome Components: Midpoint Redoxpotentials, Light- and NADH Mediated Reductions....Pages 395-396 Localisation of Donor and Acceptor Sites of NAD(P)H Dehydrogenase(S) Using “Inside-Out” and “Right-Side-Out” Plant Plasma Membrane Vesicles, and Characterization of Plasma Membrane-Bound b-Cytochromes....Pages 397-398 NADH-Ferricyanide Oxidoreductase Activity in Maize Root Cell Plasma Membrane....Pages 399-399 The Effect of Sethoxydim on the NAD(P)H Oxidation Associated with Isolated Plasmalemma Vesicles....Pages 400-400 The Resolution of Two Phases of Ferricyanide Reduction in Cultured Carrot Cells....Pages 401-401 Modification of Plasma Membrane DT-Diaphorase Activity Upon Transformation: A Comparison between Astrocytes in Primary Culture and C6 Glioblastoma Cells....Pages 402-402 Transplasma — Membrane Redox System in Erythrocyte Ghosts....Pages 403-403 Stimulation of NADH-Cytochrome C Oxidoreductase of Endoplasmic Reticulum Transitional Elements by Retinol....Pages 404-405 NADH-Dehydrogenases in Synaptic Plasma Membranes....Pages 406-407 Are Plasmalemma Redox Protons Involved in Growth Control by Plant Cells?....Pages 408-408 Is the Proton Extrusion Coupled to Ferricyanide Reduction Mediated by the H + -ATPase ?....Pages 409-409 Relationship of Plasma Membrane Redox Activity to Proton Transport in Roots of Iron-Deficient Cucumber....Pages 410-411 Intracellular pH Modifications Linked to the Activity of the Ferricyanide Driven Activity of the Plasmalemma Redox System in Elodea densa Leaves, Acer pseudoplatanus and Catharanthus roseus Cells....Pages 412-412 Preliminary Evidence for a Regulation of the Plasma Membrane Redox Chain by the Activity of the ATP-Driven H + Pump in Elodea Densa Leaves....Pages 413-413 Relationship between Auxin-Dependent Proton Extrusion and Plasmalemma Redox System in Maize Coleoptile Segments....Pages 414-414 Possible Exploitation of an H+ Related Mechanism in the Treatment of Breast and Colon Tumors: A New Approach to the Adjuvant Treatment of Cancer....Pages 415-418 Control of Sodium/Proton Exchange by Plasma Membrane Electron Transport....Pages 419-420 Nitrate Uptake by Intact Sunflower Plants....Pages 421-421 Metabolic Changes Associated with Ferricyanide Reduction by Elodea Leaves....Pages 422-422 Stimulation of C-fos and C-myc Proto-Oncogene Expression by Ferricyanide, an Extracellular Electron Acceptor....Pages 423-423 Effeects of PMA on Protein Kinase C in Perfused Rat Heart....Pages 424-425 Role of Zinc in Plasma Membrane Integrity and O 2 − -Generating NADPH Oxidase Activity....Pages 426-426 Anion Superoxide and Hydrogen Peroxide Formation Elicited by NAD(P)H Oxidation in Radish Plasmalemma Vesicles....Pages 427-427 Ascorbate Free Radical Involvement in Elongation-Related Growth Processes in Onion Roots....Pages 428-428 Role of Cell Surface on NADH-MDHA-Oxidoreductase: Lectin Inhibition....Pages 429-429 Iron Reduction by Sunflower Roots under Iron Stress....Pages 430-430 Iron-Stress in Tomato ( Lycopersicum esculentum cv. Rutgers) Results in Increased Iron Reduction on the Root Plasma Membrane....Pages 431-431 Back Matter....Pages 433-443