Plant Protoplasts And Genetic Engineering Ii (biotechnology In Agriculture And Forestry)

Front Matter....Pages I-XX Genetic Engineering and In Vitro Manipulation of Plant Cells — Technical Advances....Pages 1-25 Genetic Manipulation: Generative Versus Somatic....Pages 26-53 Uptake and Integration of Exogenous DNA in Plants....Pages 54-74 DNA Recombinants and Transformation of Agricultural Crops....Pages 75-98 Genetically Transformed Maize ( Zea mays L.) Plants from Protoplasts....Pages 99-106 Protoplast Culture and Transformation Studies on Rice....Pages 107-121 Transformation in Potato....Pages 122-139 Transformation in Cotton....Pages 140-154 Transformation in Populus spp.....Pages 155-172 Transformation in Vinca rosea L.: An Example of Spheroplast Method for Gene Transfer....Pages 173-187 Transformation in Hyoscyamus ....Pages 188-196 Genetic Manipulation in Brassica ....Pages 197-216 Direct Gene Transfer in Protoplasts of Nicotiana plumbaginifolia ....Pages 217-227 Microinjection of Cells and Protoplasts: Integration of Foreign DNA....Pages 228-240 Electrofusion: The Technique and Its Application to Somatic Hybridization....Pages 241-256 Versatile Apparatuses for Electrogene Mapping, Electrophoresis and Electrofusion....Pages 257-282 Chloroplast Genomes as Genetic Markers....Pages 283-303 Flow Cytometric Analysis and Sorting of Somatic Hybrid and Transformed Protoplasts....Pages 304-327 Isolation of Nuclei and Their Transplantation into Plant Protoplasts....Pages 328-342 Chromosome Transplantation and Applications of Flow Cytometry in Plants....Pages 343-359 Transfer of Mitochondria Through Protoplast Fusion....Pages 360-375 Incorporation of the Firefly Luciferase Gene into Plant Cells....Pages 376-387 Uptake of Viruses by Plant Protoplasts and Their Use as Transforming Agents....Pages 388-405 Uptake of Fungal Protoplasts by Plant Protoplasts....Pages 406-427 Chloroplast Uptake and Genetic Complementation....Pages 428-446 Cytoplasts: Isolation and Uses....Pages 447-458 Vacuoles: Isolation, Purification, and Uses....Pages 459-479 Miniprotoplasts and Their Use in Genetic Engineering....Pages 480-493 Back Matter....Pages 495-499