Plant hormone receptors [proceedings of the NATO Advanced Research Workshop on Plant Hormone Receptors held at Bad Honnef (Bonn), FRG, August 18 - 22, 1986

The Nato Advanced Research Workshop on Plant Hormone Receptors was held at the Physik Zentrum in Bad Honnef near Bonn, August 18-22, 1986. This workshop was mainly supported by the Nato Scientific Affairs Division and additionally cosponsered by Hoechst AG, Frankfurt and BASF AG, Ludwigshafen. The workshop aimed at focusing research on plant hormone recep­ tors. It should provide an opportunity to all who work in this field to report on their very recent data and to discuss their results with the most competent' colleagues. The total number of participants was limited to 30 to ensure personal contact and intensive discussions. Everyone had to either give a lecture or practical course. One half of the participants were invited, the other was selected by applications. Plant hormone receptors are assumed to exist but clear results are still rare. Nevertheless encouraging results have been published over the last years. Receptors for animal hormones and neuronal transmitters are well characterized, both structu­ rally and functionally. Therefore scientists dealing with recep­ tors for steroid hormones - Prof. E.E. Baulieu, Paris and Prof. J. R. Gustafsson, Huddinge - and for acetylcholine - Prof. A. Maelicke, Dortmund - were invited to participate in the workshop. Front Matter....Pages I-XII Too Many Binding Proteins, not Enough Receptors?....Pages 1-11 Hormone Recognition in Plants....Pages 13-26 Can Auxin Receptors be Purified by Affinity Chromatography?....Pages 27-39 The Auxin Receptor in Corn Coleoptiles....Pages 41-49 Cytosolic and Membrane-Bound High-Affinity Auxin-Binding Proteins in Tobacco....Pages 51-62 Soluble Auxin-Binding: Is there a Correlation between Growth-Stage Dependent High-Affinity Auxin-Binding and Auxin Competence?....Pages 63-69 Phytohormone-Receptors from Tobacco Crown Gall Tissues....Pages 71-79 Auxin Transport: Binding of Auxins and Phytotropins to the Carriers. Accumulation into and Efflux from Membrane Vesicles....Pages 81-92 Preliminary Separation of Pea Stem NPA Receptors by High Performance Ion Exchange Chromatography....Pages 93-97 Effect of Surface and Membrane Potentials on IAA Uptake and Binding by Zucchini Membrane Vesicles....Pages 99-112 Modifications of Auxin Efflux Carrier in the Auxin Transport System by Diethyl Ether and Ethylene....Pages 113-123 Search for Endogenous Ligands to Fusicoccin Binding Sites....Pages 125-130 The Interaction of Fusicoccin with Specific Binding Sites....Pages 131-139 About the Search for the Molecular Action of High-Affinity Auxin-Binding Sites on Membrane-Localized Rapid Phosphoinositide Metabolism in Plant Cells....Pages 141-153 Hormone Receptor Manipulation by Hydrostatic Pressure: Interaction between Ca 2+ , Membrane Components and Phosphatidylinositol in Pea Foliage Microsomes....Pages 155-162 Stimulation of In Vitro H + Transport in Zucchini Microsomes by the Ether Lipid Platelet Activating Factor and a Soluble Protein....Pages 163-175 Immunocytological Localization of a Wheat Embryo Cytokinin Binding Protein and its Homology with Proteins in other Cereals....Pages 177-184 Studies on Cytokinin Binding Proteins....Pages 185-197 The Gibberellin Receptor....Pages 199-227 Ethylene Binding Sites....Pages 229-238 Ethylene Binding and Evidence that Binding In Vivo and In Vitro is to the Physiological Receptor....Pages 239-248 Ethylene-Induced Growth in Amphibious Plants....Pages 249-256 Back Matter....Pages 259-319 The NATO Advanced Research Workshop on Plant Hormone Receptors, held in Bad Honnef in August 1986, provided an opportunity for scientists working in this field to present their most recent data and to discuss their results with the most competent of their colleagues. The report contains short reviews and new results of the knowledge on plant hormone recognition by plant cells and on the molecular mechanism of their action. The contributions include: - cytosolic and membrane associated auxin receptors and their possible functions, - auxin transport carriers, - cytokinin binding proteins, - ethylene binding proteins, - gibberellin binding proteins, - fusicoccin binding proteins. The report includes a methodical appendix for the most useful methods, employed in research work within this field Proceedings of the NATO Advanced Research Workshop on Plant Hormone Receptors held at Bad Honnef (Bonn), FRG, August 18- 22, 1986