Pharmacology of Hormonal Polypeptides and Proteins : Proceedings of an International Symposium on the Pharmacology of Hormonal Polypeptides, Held in Milan, Italy, September 14–16, 1967

It can be concluded (under the specific experimental procedures em­ ployed) that:- 1) HCG labelled with 1 - 2 atoms of radioactive iodine did not differ sig­ nificantly from the unlabelled hormone; 2) The ovary alone exhibited a capacity to affix specifically HCG; 3) The amount of radioactive material in the ovary was directly proport­ ional to the quantity of labelled HCG injected; 4) When the HCG present in the circulation is bound to an antiserum to HCG, the antigen-antibody complex is not concentrated by the ovary; 5) Circulating labelled HCG decreased to 50% within 30 minutes following a single intravenous injection; 6) There are four different phases of ovarian uptake of HCG, namely:­ the first phase, when there is only an inflow from the circulation and stor­ age mainly in the follicular envelopes; the second phase, when there is a greater inflow than outflow; the third phase, when the inflow is equal to the outflow; and the fourth period, when the outflow is bigger than the in­ flow. REFERENCES 1. Lunenfeld, B. and Eshkol, A. Vitamins and Hormones (1967) 25:165 2. Eshkol, A. In: Recent Research on Gonadotropbio Hormones, eds. E. T. Bell andJ. A. Loraine, Edinburgh, Livingstone (1967), p. 202. 3. Eshkol, A. and Lunenfeld, B. Proc. Tel-Hashomer Hosp. (1967) 6:4. ACKNOWLEOOMEN'IS This work was supported in part by a grant from the Population Council, N. Y. , U. S. A. andbyGrantNo. Front Matter....Pages i-xi Some New Methods in Peptide Synthesis....Pages 1-10 Novel Techniques in Peptide Synthesis....Pages 11-16 Synthetic polypeptides with enhanced biological activities....Pages 17-17 Synthesis of Gastrointestinal Hormones....Pages 18-31 The Synthesis of Caerulein....Pages 32-34 Techniques for the Synthesis of Acth And Msh Peptides and Analogues....Pages 35-47 Studies with Tryptophan in Solid-Phase Synthesis....Pages 48-52 Oxytocin — Molecular Aspects....Pages 53-65 Oxytocin and Vasopressin: Biochemical Considerations....Pages 66-72 Neurophysin, Oxytocin and Vasopressin in Neurosecretory Granules and in Crystalline Complexes....Pages 73-83 Mechanism of Release and Mechanism of Action of Vasopressin....Pages 84-92 Radioimmunoassay of Oxytocin....Pages 93-100 Comparison of the Pharmacological Properties of Two Highly Potent, Crystalline Neurohypophyseal Hormone Analogs: Deamino-1-Seleno-Oxytocin and Deamino-Oxytocin....Pages 101-104 Structure and Function of Pituitary Grafts, Related to the Problem of Hypothalamic Control of Hypophyseal Activity....Pages 105-111 The Physiology and Biochemistry of Luteinizing Hormone-Releasing Factor and Follicle Stimulating Hormone-Releasing Factor....Pages 112-122 Studies on the Isolation of Luteinizing Hormone Releasing Factor (LRF)....Pages 123-127 Studies on Luteinizing Hormone Releasing Factor (LRF) in Hypophysial Portal Blood of Rats....Pages 128-137 Studies on the Mechanism of Action of Hypothalamic FSH- and LH-Releasing Factors....Pages 138-147 Thyrotropin-Releasing Factor (TRF) of Hypothalamic Origin. A Review of Physiological and Biochemical Studies....Pages 148-157 Physiological and Biochemical Studies on Some Highly Purified Hypothalamic Releasing Factors....Pages 158-166 Role of the Cerebral Cortex in the Control of Acth Secretion....Pages 167-174 The Pharmacological Approach to the Study of the Mechanisms Regulating Acth Secretion....Pages 175-189 Effects of ACTH- and MSH-Peptides on Central Nervous System....Pages 190-195 Pharmacology of Synthetic Acth Peptides of Different Chain Lengths....Pages 196-202 Purification of Porcine and Human ACTH....Pages 203-212 The Mechanism of Action of Luteinizing Hormone on Steroidogenesis in the Corpus Luteum in Vitro ....Pages 213-222 Fate and Localization of Iodine-Labelled HCG in Mice....Pages 223-228 Human Follicle-Stimulating Hormone. Purification and Some Biological Properties....Pages 229-238 The Trend of Androgen Metabolites After Ovulation Induced by FSH and LH Fractions or by HCG Alone....Pages 239-241 Some Factors Influencing Suckling-Induced Prolactin Release in the Lactating Rat....Pages 242-253 Production of a Thyroid Stimulator by Immunizing Rabbits with Human Thyroid....Pages 254-262 A Comparative Study on the Mouse Thyroid Ultrastructure after Stimulation with TSH and LATS....Pages 263-270 Vasopressin Effect on Cortisol Incretion in Man....Pages 271-274 Pseudopregnancy after Monoamine Depletion in the Median Eminence of the Rat....Pages 275-277 The Synthesis of Insulin....Pages 278-296 Effects of Digestive Secretagogues on the Endocrine Pancreas in Man....Pages 297-303 Serum Insulin Response to Glucagon as an Index of Insulin Reserve....Pages 304-312 The Effect of Glucose, Glucagon, Arginine, Vasopressin and ACTH on Plasma Insulin and Growth Hormone Concentrations in Normal Children and in Pituitary Insufficiency....Pages 313-316 Insulin Secretion “In Vitro”: Comparative Aspects and Comparisons with Studies on Release of Insulin “In Vivo”....Pages 317-335 Reactivity of -S-S- Bonds in Relation with Insulin Biological Activity and Catabolism....Pages 336-350 The Physiologic Role of Glucagon....Pages 351-360 Sodium Dependency of Insulin-Stimulated Glucose Transport in Isolated Fat Cells.....Pages 361-366 The Role of Cyclic AMP in the Action of Insulin....Pages 367-376 Stimulation of Active Sodium Transport by Insulin....Pages 377-381 Distinct antagonistic effects of glucagon and insulin in the Embden-Meyerhof-chain....Pages 382-386 Selective Histochemistry of Glucagon in the a Cells of Pancreatic Islets by Indole Methods....Pages 387-395 Interrelationships Glucagon-Adrenergic System in Man Interest for the Diagnosis of Pheochromocytoma....Pages 396-400 Evolutionary Changes in Adipose Tissue Physiology....Pages 401-415 Aspects of Glucagon-Induced Lipolysis in Adipose Tissue....Pages 416-424 The Lipolytic Action of Catecholamines and Acth and the Interaction of Prostaglandin E 1 .....Pages 425-439 Lipid Mobilizing Peptide in Hog Pituitary and Human Urine....Pages 440-448 Study of the Lipolytic Effect of ACTH in Isolated Fat Cells of the Rat: Potentialization of the Lipolytic Effect by Adding Small Quantities of Serum of Guinea-Pig, Rat and Normal Man....Pages 449-455 The Anabolic Effects of Pituitary Growth Hormone....Pages 456-462 The Subcellular Distribution of Human Placental Lactogen....Pages 463-472 The Biological Characterization of Purified Placental Protein (Human) [PPP(H)]....Pages 473-482 Calcitonin....Pages 483-485 Biochemical Aspects of Hypotensive Peptide Action....Pages 486-496 Pharmacology of Angiotensin Antagonists....Pages 497-506 Effects of Angiotensin on Na and K Exchanges in Blood Vessels....Pages 507-516 Variations in the Aldosterone-Stimulating Activity of Angiotensin: Causes and Physiological Significance....Pages 517-526 Isolation and Structure of Caerulein....Pages 527-532 Pharmacological Data on Caerulein....Pages 533-540 “Vasodilating Action of Caerulein and I.C.I. 50,123 on the Canine Pancreatic Vascular Bed”....Pages 541-543 Studies on an Urinary Protein Factor of Gastric Antiulcer Activity (Urogastrone)....Pages 544-552 Biogenesis of Erythropoietin....Pages 553-562 Physico-Chemical and Biological Characteristics of Erythropoietin....Pages 563-568 Secretin, Cholecystokinin....Pages 569-580 Relationship between Behavioural Effect and Circulatory Changes Produced by Intra-Carotid Bradykinin....Pages 581-589 The Inflammatory Response and Polypeptides....Pages 590-594 Kinins in Chronic Inflammation....Pages 595-600 Adrenergic Receptors and Kinins....Pages 601-610 The Formation, Actions and Properties of Anaphylatoxin....Pages 611-619 Cholinergic Effects of Angiotensin and Bradykinin....Pages 620-625 On the Mechanism of Bradykinin Potentiation....Pages 626-631 Mechanism of Action of Relaxin....Pages 632-638 Comments on the Role of Plasma Kinins in Inflammation....Pages 639-640 Back Matter....Pages 641-660 It can be concluded (under the specific experimental procedures emƯ ployed) that:- 1) HCG labelled with 1 - 2 atoms of radioactive iodine did not differ sigƯ nificantly from the unlabelled hormone; 2) The ovary alone exhibited a capacity to affix specifically HCG; 3) The amount of radioactive material in the ovary was directly proportƯ ional to the quantity of labelled HCG injected; 4) When the HCG present in the circulation is bound to an antiserum to HCG, the antigen-antibody complex is not concentrated by the ovary; 5) Circulating labelled HCG decreased to 50% within 30 minutes following a single intravenous injection; 6) There are four different phases of ovarian uptake of HCG, namely:Ư the first phase, when there is only an inflow from the circulation and storƯ age mainly in the follicular envelopes; the second phase, when there is a greater inflow than outflow; the third phase, when the inflow is equal to the outflow; and the fourth period, when the outflow is bigger than the inƯ flow. REFERENCES 1. Lunenfeld, B. and Eshkol, A. Vitamins and Hormones (1967) 25:165 2. Eshkol, A. In: Recent Research on Gonadotropbio Hormones, eds. E.T. Bell andJ. A. Loraine, Edinburgh, Livingstone (1967), p. 202. 3. Eshkol, A. and Lunenfeld, B. Proc. Tel-Hashomer Hosp. (1967) 6:4. ACKNOWLEOOMEN'IS This work was supported in part by a grant from the Population Council, N.Y., U.S.A. andbyGrantNo