Pharmacological Assays of Plant-Based Natural Products (Progress in Drug Research Book 71)

1. Extraction of plant material -- 2. Critical aspects of purification and impurities in natural product extracts. 3. In vitro antioxidant assays -- 4. In vivo antioxidant assays -- 5. Quantification assays -- 6. Anti-nutritional factors -- 7. Anti-inflammatory activity -- 8. Antipyretic activity -- 9. Inflammation bowel disease -- 10. Anti-arthritic activity -- 11. Evaluation of analgesic potential -- 12. Anti-diarrhoeal activity -- 13. Anti-ulcer activity -- 14. In vitro anti-diabetic activity -- 15. In vivo anti-diabetic activity -- 16. Hepatoprotective property -- 17. Antitumor activity -- 18. In vivo wound healing studies -- 19. Determination of cytotoxicity -- 20. Evaluation of bioavailability -- 21. Molecular identification by DNA barcoding -- 22. Molecular docking of bioactive compounds against BRCA and COX proteins.;This volume provides information on how to select and screen plants for their medicinal properties. It describes phytopharmacological techniques for extracting and qualitatively and quantitatively analyzing a plantâ#x80;#x99;s phytochemicals. After a detailed in vitro investigation including nutritional and anti-nutritional analyses, medicinal properties were tested with various in vivo models for anti-inflammatory, analgesic, anti-pyretic, anticancer and anti-diabetic properties, as well as wound healing, neurodegenerative diseases, etc. Compound identification and purification techniques include, among others, TLC and column chromatography, as well as molecular docking with specific proteins. Front Matter....Pages i-xv Ethnobotanical Study....Pages 1-4 Pharmacognostical Studies....Pages 5-10 Extraction of Bioactive Compounds....Pages 11-13 Preliminary Phytochemical Studies....Pages 15-19 Proximate Composition Analysis....Pages 21-31 Mineral Quantification....Pages 33-41 Anti-nutritional Factors....Pages 43-47 Quantification of Secondary Metabolites....Pages 49-55 In Vitro Antioxidant Assays....Pages 57-72 In Vitro Antihaemolytic Activity....Pages 73-75 Antimicrobial Activity....Pages 77-78 In Vitro Anthelmintic Assay....Pages 79-80 Toxicity Studies....Pages 81-87 In Vivo Antioxidant Assays....Pages 89-98 Analgesic Activity....Pages 99-102 Anti-inflammatory Activity....Pages 103-111 Antipyretic Activity....Pages 113-115 Inflammatory Bowel Disease....Pages 117-122 In Vitro Anti-arthritic Activity....Pages 123-127 Anti-diarrhoeal Activity....Pages 129-130 Anti-ulcer Activity....Pages 131-133 Hepatoprotective Activity....Pages 135-137 Anti-diabetic Activity....Pages 139-143 Evaluation of Anti-diabetic Property on Streptozotocin-Induced Diabetic Rats....Pages 145-149 In Vivo Wound Healing Studies....Pages 151-157 Determination of Cytotoxicity....Pages 159-161 Induction of Apoptosis....Pages 163-164 Anti-tumour Activity....Pages 165-167 Protein Bioavailability in Animal Model....Pages 169-172 Detection of Phenolic and Flavonoid Compounds Using High Performance Thin Layer Chromatography (HPTLC)....Pages 173-175 Isolation of Compounds....Pages 177-180 Molecular Docking of Bioactive Compounds Against BRCA and COX Proteins....Pages 181-183 Back Matter....Pages 185-188 "This volume provides information on how to select and screen plants for their medicinal properties. It describes phytopharmacological techniques for extracting and qualitatively and quantitatively analyzing a plant?s phytochemicals. After a detailed in vitro investigation including nutritional and anti-nutritional analyses, medicinal properties were tested with various in vivo models for anti-inflammatory, analgesic, anti-pyretic, anticancer and anti-diabetic properties, as well as wound healing, neurodegenerative diseases, etc. Compound identification and purification techniques include, among others, TLC and column chromatography, as well as molecular docking with specific proteins"--Page 4 of cover