Pharmaceutical and Biomedical Applications of Capillary Electrophoresis

The book describes the theory and applications of Capillary Electrophoresis (CE) in the field of pharmaceutical and biomedical analysis. It is targeted towards users who are intimately involved in analytical problems, especially those which involve small samples. This book presents the technique of capillary electrophoresis from the point of view of the serious hands-on use in the field of pharmaceutical and biomedical analysis. An overview of general theory is presented to acquaint the novice with the fundamental principles. A more theoretical approach is taken in the presentation of electrokinetic chromatography. The next chapter discusses advances in column technologies, the preceding chapters having provided a foundation as to how separations occur. In the next three chapters, recognized experts in their fields present fundamentals and state-of-the-art techniques in the areas of optical, electrochemical and mass spectrometric detection. The major focus of the remaining chapters is on applications. This includes the analysis of pharmaceuticals, amino acids and peptides, macromolecules, nucleosides, nucleotides and oligonucleotides. The use of CE for analysis of small ions and separation of biological particles is also discussed. The issue of sample preparation for analysis by CE is addressed, especially as it relates to clinical analysis. Content: List of contributors Pages vii-viii Introduction Pages ix-x Susan M. Lunte, Donna M. Radzik Chapter 1 Principles of capillary electrophoresis Original Research Article Pages 3-21 Susan M. Lunte, Donna M. Radzik Chapter 2 Column technologies for capillary electrophoresis Original Research Article Pages 23-80 John K. Towns, Fred E. Regnier Chapter 3 Electrokinetic chromatography Original Research Article Pages 81-178 Joseph P. Foley, Eric S. Ahuja Chapter 4 Optical detection strategies in capillary electrophoresis Original Research Article Pages 181-228 Steven A. Soper Chapter 5 Capillary electrophoresis/Mass spectrometry Original Research Article Pages 229-275 Richard D. Smith, Harold R. Udseth Chapter 6 Electrochemical detection for capillary electrophoresis Original Research Article Pages 277-306 Thomas J. O'shea Chapter 7 Sample preparation for capillary electrophoresis Original Research Article Pages 309-326 David K. Lloyd Chapter 8 Capillary electrophoresis in the study of amino acids Original Research Article Pages 327-344 Paul L. Weber Chapter 9 Capillary electrophoresis of proteins and peptides Original Research Article Pages 345-386 Kathleen L. Kostel Chapter 10 Separation of nucleic acids and oligonucleotides and sequencing of DNA Fragments by capillary electrophoresis Original Research Article Pages 387-424 Ram P. Singhal, Jun Xian Chapter 11 The Use of Capillary Electrophoresis in Pharmaceutical Development Original Research Article Pages 425-466 Sou Chan Chang, John J. Gigantino, Donna M. Radzik, Allen C. Rychtman Chapter 12 Capillary electrophoresis-based methods for in vivo analysis: Single cell analysis and microdialysis sampling Original Research Article Pages 467-502 Barry L. Hogan Index Pages 503-511 This text describes the theory and applications of Capillary Electrophoresis (CE) in the field of pharmaceutical and biomedical analysis. It is aimed at readers who are intimately involved in analytical problems, especially those which involve small samples.