Perspectives on Fluorescence: A Tribute to Gregorio Weber (Springer Series on Fluorescence Book 17)

Gregorio Weber is widely acknowledged as the person responsible for the advent of modern fluorescence spectroscopy. Since 2016 is the 100 th anniversary of Gregorio Weber’s birth, this special volume has been prepared to honor his life and achievements. It offers contributions from outstanding researchers in the fluorescence field, describing their perspectives on modern fluorescence and its highly diverse applications, ranging from the photophysics of tryptophan and proteins, membrane studies, fluorescence microscopy on live cells, novel software approaches and instrumentation. Many of the authors knew Gregorio Weber personally and have shared their impressions of the man and his contributions. This volume appeals not only to aficionados of fluorescence spectroscopy and its applications in biology, chemistry and physics, but also to those with a general interest in the historical development of an important scientific field. Front Matter....Pages i-x A Fluorescent Lifetime: Reminiscing About Gregorio Weber....Pages 1-16 Gregorio Weber’s Roots in Argentina....Pages 17-40 The Labyrinthine World of Gregorio Weber....Pages 41-56 Personal Recollections of Gregorio Weber, My Postdoc Advisor, and the Important Consequences for My Own Academic Career....Pages 57-66 Measurements of Fluorescence Decay Time by the Frequency Domain Method....Pages 67-80 Ultra-Fast Fluorescence Anisotropy Decay of N-Acetyl-l-Tryptophanamide Reports on the Apparent Microscopic Viscosity of Aqueous Solutions of Guanidine Hydrochloride....Pages 81-94 Weber’s Red-Edge Effect that Changed the Paradigm in Photophysics and Photochemistry....Pages 95-141 Imaging Lifetimes....Pages 143-161 The Impact of Laser Evolution on Modern Fluorescence Spectroscopy....Pages 163-178 Effects of Sterol Mole Fraction on Membrane Lateral Organization: Linking Fluorescence Signals to Sterol Superlattices....Pages 179-196 The Use of 6-Acyl-2-(Dimethylamino)Naphthalenes as Relaxation Probes of Biological Environments....Pages 197-216 Continuing Inspiration: Gregorio Weber’s Influence on Understanding the Basis of Allosteric Regulation of Enzymes....Pages 217-233 Using Fluorescence to Characterize the Role of Protein Oligomerization in the Regulation of Gene Expression....Pages 235-254 Light Initiated Protein Relaxation....Pages 255-270 Synthetic and Genetically Encoded Fluorescence Probes for Quantitative Analysis of Protein Hydrodynamics....Pages 271-286 Spatiotemporal Fluorescence Correlation Spectroscopy of Inert Tracers: A Journey Within Cells, One Molecule at a Time....Pages 287-309 Role of the Pico-Nano-Second Temporal Dimension in STED Microscopy....Pages 311-329 Plasma Membrane DC-SIGN Clusters and Their Lateral Transport: Role in the Cellular Entry of Dengue Virus....Pages 331-342 Back Matter....Pages 343-346 "Gregorio Weber is widely acknowledged as the person responsible for the advent of modern fluorescence spectroscopy.Since 2016 is the 100th anniversary of Gregorio Weber's birth, this special volume has been prepared to honor his life and achievements. It offers contributions from outstanding researchers in the fluorescence field, describing their perspectives on modern fluorescence and its highly diverse applications, ranging from the photophysics of tryptophan and proteins, membrane studies, fluorescence microscopy on live cells, novel software approaches and instrumentation. Many of the authors knew Gregorio Weber personally and have shared their impressions of the man and his contributions. This volume appeals not only to aficionados of fluorescence spectroscopy and its applications in biology, chemistry and physics, but also to those with a general interest in the historical development of an important scientific field." -- Orbis Yale University Library