Nuclear reprogramming : methods and protocols

__Nuclear Reprogramming: Methods and Protocols, Second Edition__ includes not only classic methods to perform nuclear transfer in different species but also several techniques to assess the early and late development of the reconstructed embryos, at the cellular, molecular, and epigenetic level. Over the past several years, many technical improvements have been made to improve somatic cell nuclear transfer (SCNT) efficiency, all of which are reflected in the detailed chapters of this fully revised collection. Written in the highly successful __Methods in Molecular Biology__ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, __Nuclear Reprogramming: Methods and Protocols, Second Edition__ will be of interest not only to cloners but also to researchers concerned with studying the development of embryos. Front Matter....Pages i-xi Back Matter....Pages 1-14 ....Pages 15-23